LOCUS       sequence-350183-        4151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning 
            ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK036
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatgcct caatcctggg aagaactggc cgctgataag cgcgcccgcc
     2281 tcgcaaaaac catccctgat gaatggaaag tccagacgct gcctgcggat gacagcgtta
     2341 ttgatttccc aaagaaatcg gggatccttt cagaggccga actgaagatc acagaggcct
     2401 ccgctgcaga tcttgtgtcc aagctggcgg ccggagagtt gacctcggtg gaagttacgc
     2461 tagcattctg taaacgggca gcaatcgccc agcagttagt agggtcccct ctacctctca
     2521 gggagatgta acaacgccac cttatgggac tatcaagctg acgctggctt ctgtgcagac
     2581 aaactgcgcc cacgagttct tccctgacgc cgctctcgcg caggcaaggg aactcgatga
     2641 atactacgca aagcacaaga cacccgttgg tccactccat ggcctcccca tctctctcaa
     2701 agaccagctt cgagtcaagg tacaccgttg cccctaagtc gttagatgtc cctttttgtc
     2761 agctaacata tgccaccagg gctacgaaac atcaatgggc tacatctcat ggctaaacaa
     2821 gtacgacgaa ggggactcgg ttctgacaac catgctccgc aaagccggtg ccgttttcta
     2881 cgtcaagacc tctgtcccgc agaccctgat ggtctgcgag acagtcaaca acatcatcgg
     2941 gcgcaccgtc aacccacgca acaagaactg gtcgtgcggc ggcagttctg gtggtgaggg
     3001 tgcgatcgtt gggattcgtg gtggcgtcat cggtgtagga acggatatcg gtggctcgat
     3061 tcgagtgccg gccgcgttca acttcctgta cggtctaagg ccgagtcatg ggcggctgcc
     3121 gtatgcaaag atggcgaaca gcatggaggg tcaggagacg gtgcacagcg ttgtcgggcc
     3181 gattacgcac tctgttgagg gtgagtcctt cgcctcttcc ttcttttcct gctctatacc
     3241 aggcctccac tgtcctcctt tcttgctttt tatactatat acgagacggg cagtcactga
     3301 tgaagtaatg ttagacctcc gcctcttcac caaatccgtc ctcggtcagg agccatggaa
     3361 atacgactcc aaggtcatcc ccatgccctg gcgccagtcc gagtcggaca ttattgcctc
     3421 caagatcaag aacggcgggc tcaatatcgg ctactacaac ttcgacggca atgtccttcc
     3481 acaccctcct atcctgcgcg gcgtggaaac caccgtcgcc gcactcgcca aagccggtca
     3541 taccgtgacc ccgtggacgc catacaagca cgatttcggc cacgatctca tctcccatat
     3601 ctacgcggct gacggcagcg ccgacgtaat gcgcgatatc agtgcatccg gcgagccggc
     3661 gattccaaat atcaaagacc tactgaaccc gaacatcaaa gctgttaaca tgaacgagct
     3721 ctgggacacg catctccaga agtggaatta ccagatggag taccttgaga aatggcggga
     3781 ggctgaagaa aaggccggga aggaactgga cgccatcatc gcgccgatta cgcctaccgc
     3841 tgcggtacgg catgaccagt tccggtacta tgggtatgcc tctgtgatca acctgctgga
     3901 tttcacgagc gtggttgttc cggttacctt tgcggataag aacatcgata agaagaatga
     3961 gagtttcaag gcggttagtg agcttgatgc cctcgtgcag gaagagtatg atccggaggc
     4021 gtaccatggg gcaccggttg cagtgcaggt tatcggacgg agactcagtg aagagaggac
     4081 gttggcgatt gcagaggaag tggggaagtt gctgggaaat gtggtgactc cataggcttt
     4141 gagaccacga a
//
