LOCUS       sequence-35019-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Jnk3a1
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccaat ggcagcaaaa
      121 gcaaagttga caaccagttc tacagtgtgg aagtgggaga ctcaaccttc acagttctca
      181 agcgctacca gaatctaaag cctattggct ctggggctca gggcatagtt tgtgccgcgt
      241 atgatgctgt ccttgacaga aatgtggcca ttaagaagct cagcagaccc tttcagaacc
      301 aaacacatgc caagagagcg taccgggagc tggtcctcat gaagtgtgtg aaccataaaa
      361 acattattag tttattaaat gtcttcacac cccagaaaac gctggaggag ttccaagatg
      421 tttacttagt aatggaactg atggatgcca acttatgtca agtgattcag atggaattag
      481 accatgagcg aatgtcttac ctgctgtacc aaatgttgtg tggcattaag cacctccatt
      541 ctgctggaat tattcacagg gatttaaaac caagtaacat tgtagtcaag tctgattgca
      601 cattgaaaat cctggacttt ggactggcca ggacagcagg cacaagcttc atgatgactc
      661 catatgtggt gacacgttat tacagagccc ctgaggtcat cctggggatg ggctacaagg
      721 agaacgtgga tatatggtct gtgggatgca ttatgggaga aatggttcgc cacaaaatcc
      781 tctttccagg aagggactat attgaccagt ggaataaggt aattgaacaa ctaggaacac
      841 catgtccaga attcatgaag aaattgcaac ccacagtaag aaactatgtg gagaatcggc
      901 ccaagtatgc gggactcacc ttccccaaac tcttcccaga ttccctcttc cca
//
