LOCUS       sequence-35020-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Erk1
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccaat gcggcggcgg cggctcaggg
      121 gggcgggggc ggggagcccc gtagaaccga gggggtcggc ccgggggtcc cgggggaggt
      181 ggagatggtg aaggggcagc cgttcgacgt gggcccgcgc tacacgcagt tgcagtacat
      241 cggcgagggc gcgtacggca tggtcagctc ggcctatgac cacgtgcgca agactcgcgt
      301 ggccatcaag aagatcagcc ccttcgaaca tcagacctac tgccagcgca cgctccggga
      361 gatccagatc ctgctgcgct tccgccatga gaatgtcatc ggcatccgag acattctgcg
      421 ggcgtccacc ctggaagcca tgagagatgt ctacattgtg caggacctga tggagactga
      481 cctgtacaag ttgctgaaaa gccagcagct gagcaatgac catatctgct acttcctcta
      541 ccagatcctg cggggcctca agtacatcca ctccgccaac gtgctccacc gagatctaaa
      601 gccctccaac ctgctcatca acaccacctg cgaccttaag atttgtgatt tcggcctggc
      661 ccggattgcc gatcctgagc atgaccacac cggcttcctg acggagtatg tggctacgcg
      721 ctggtaccgg gccccagaga tcatgctgaa ctccaagggc tataccaagt ccatcgacat
      781 ctggtctgtg ggctgcattc tggctgagat gctctctaac cggcccatct tccctggcaa
      841 gcactacctg gatcagctca accacattct gggcatcctg ggctccccat cccaggagga
      901 cctgaattgt atcatcaaca tgaaggcccg aaactaccta cagtctctgc c
//
