LOCUS       sequence-350204-       15491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 based expression in filamentous fungi pYFAC-riboB carrying 
            PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction 
            site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for 
            PaldA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, 
            Karton A, Piggott AM, Chooi YH
  TITLE     Heterologous biosynthesis of elsinochrome A sheds light on the 
            formation of the photosensitive perylenequinone system.
  JOURNAL   Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. 
            eCollection 2019 Feb 7.
  PUBMED    30809363
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c8sc02870b"; journalName: "Chem Sci"; date: "2018-11-22- 
            22"; volume: "10"; issue: "5"; pages: "1457-1465"
COMMENT     Sequence Label: pYFAC-CH3
FEATURES             Location/Qualifiers
     source          1..15491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(159..178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      200..5448
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     5618..5639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(12140..12208,12209..13000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12358..12377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13171..13759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13660..13679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    14343..14376
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(14455..15258)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(15259..15479)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
       61 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      121 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
      181 ctttctcgcc acgttcgcca gcttattttt tgtatactgt tttgtgatag cacgaagttt
      241 ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat caaattaata
      301 agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag tttataaatg
      361 cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga tagaaaacct
      421 agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc ccgagaggtt
      481 aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa aaacaagaca
      541 gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat
      601 gataccacag tctagttgac aactagatag attctagatt gaggccaaag tctctgagat
      661 ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa
      721 taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg
      781 ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac
      841 catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat
      901 gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag
      961 aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag
     1021 ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga
     1081 actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt
     1141 ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg
     1201 atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt
     1261 agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc
     1321 ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg
     1381 atctgcataa agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa
     1441 tgtaacttgc ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag
     1501 tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta
     1561 gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc
     1621 accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa
     1681 actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt
     1741 agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg
     1801 agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt
     1861 cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc
     1921 atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt gtccttcaag
     1981 tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca
     2041 gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga
     2101 cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg
     2161 cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct
     2221 taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc
     2281 tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg
     2341 aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc
     2401 agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg
     2461 tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa
     2521 aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa
     2581 acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc gaccactgga
     2641 ctctcaggct ttccattggg gtactaacat agccatcaaa tgccggcccg tgtttaatct
     2701 cctcgagatg gctgggctca catgcgttct actcaaaaac tcttatacct aggccctctc
     2761 ccaatcgtgt atgtttggac cttcctctct cacactttgg ataacatcaa gcatactgca
     2821 gattttgacc tagttaacaa ggcacatcat gctatcactt ccaacactct tgctgagcgc
     2881 gttctgcctt gcctttctgg ttctacgaag tatccagtcc cgcctgcaac gccgcagaaa
     2941 cgccaaaagg tggggttgtc aaccgccagc tagagctcca tcgggattct ttggcattcc
     3001 gccgttcctc ggtggtcgca gataactccc taaattccct gagctaactc taagtcgacc
     3061 atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt acctcttgac
     3121 cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta tatccaaact
     3181 ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga tacttaacct
     3241 tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc actctcacct
     3301 tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc aacgattagt
     3361 atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca ctatctcgag
     3421 gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg acccgactag
     3481 ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga tatccatcca
     3541 tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct actgctatac
     3601 tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga aacatattgg
     3661 tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac aatggacctc
     3721 ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac ttggtgacta
     3781 gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg ttctcccaac
     3841 cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc taactagtgt
     3901 attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt gtcgagaatt
     3961 cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac tttgcagcaa
     4021 cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac agccattcaa
     4081 tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc cgtgcatacc
     4141 cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa aggaaaggca
     4201 agttacattc agccccgtac ctaggatcag tgctaattta taacctctag ccactcttta
     4261 tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac
     4321 cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta gtttgggact
     4381 agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt ttggcgcttt
     4441 ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc agactcagac
     4501 aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc ttgaggccct
     4561 ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt cactaactag
     4621 actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag aaagtggtaa
     4681 gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt aaacaaggaa
     4741 aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca gggtgatggc
     4801 agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc aataactagt
     4861 ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg ataatagtag
     4921 taagttattc tagcttcaga gttataggag actagatact aactagtatt agttgcaact
     4981 aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc aactagactg
     5041 tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag tctccctaat
     5101 atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc cccttctagg
     5161 ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta gtttctaact
     5221 agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa cgtttatacg
     5281 tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta gtttataatt
     5341 agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt aacaagatac
     5401 cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctgg ctttccccgt
     5461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5521 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5641 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     5701 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaatg
     5761 gggtgacgat gagccgctct tgcatctttg tttgtattat actgtctttc ttgttacaca
     5821 taattattct agaatgcccc accgttacat acgggacaca gccatttaca tatgcatgtg
     5881 gattacgagc taacgagttc attcaaatct cagaactatc acataatcat cattccccta
     5941 tcgtcaaaga ccgtaagaca aatccggttc atgcactgaa cccattcggg tagtgagtca
     6001 tttactcagc acactcgcgc tgacgctcgt cgaacacctt caatgcctcc tcggcagcct
     6061 tgacaccact gagaaccatg gcaccgaagg tagggcccat gcggttaaag ccatcaattt
     6121 cagacagctc cataccgccg attatcaagc ccttagtaac ctcgcgggtg ttcttgacga
     6181 tggcatcctg cgctgattgt gatagttccc acttgtccgt ccgcatcggc atccgcagct
     6241 cgggatagtt ccgacctagg attggatgca tgcggaaccg cacgagggcg gggcggaaat
     6301 tgacacacca ctcctctcca cgcaccgttc aagaggtacg cgtatagagc cgtatagagc
     6361 agagacggag cactttctgg tactgtccgc acgggatgtc cgcacggaga gccacaaacg
     6421 agcggggccc cgtacgtgct ctcctacccc aggatcgcat ccccgcatag ctgaacatct
     6481 atataaagac ccccaaggtt ctcagtctca ccaacatcat caaccaacaa tcaacagttc
     6541 tctactcagt taattagaac tcttccaatc ctatcacctc gccttaatta agcggaacaa
     6601 attgaagcga gtccccgtgg agcgcgagcc cgcgagagag tcgtgcggga tgatgccatg
     6661 aagtatcacc tacattgtat gctctccaga tcgaagagga atactctgaa taagtggatt
     6721 taggcggttt ctttggtcct actatatgca cactcagatt ctgtcaattg aaaagtcagc
     6781 ccgctgtcta tcatgacttc tatctccatt aattgtcatc cagccgtgcc tctgtatggt
     6841 gcgtataccc tcgactcaca cctgtgaagt ctctgccact tagcgcagcc aaagaagatt
     6901 tagtctaaag gtctacaatc aattcaggcc gtattcaggg cggccgcttc tgctgtttct
     6961 gggctcaatg ctttggtatc tcaaaggaga ggagaagcgt ccgtggaagg caggaagacc
     7021 tacaagacct ggcactcaga aagagggcgg ttgctcttaa gtgtagggct gcgcagggga
     7081 ggaactctgg ggttgtcaaa tgcggcagac ggaaatctac attagcagcg tcaatccacc
     7141 cccttttggt gaaccataaa ggggctagtc taccccagct tcgaatcaga ataatataaa
     7201 tgcggggaaa gctttaggta tgcggacaga tccatactgg gtcctccaag agatcggccc
     7261 gcacatctga cgtggtttca aggttcgacc catgcggagc cgcattggag gatctatccc
     7321 tgtaggtacc ttagctaaag atctacgccg tatggccaag cggaagatat aggaacgatc
     7381 ggccatgaag tcgtgtcttc tacaaactct cgtatattca ctaggttcga cgtctgctaa
     7441 actaacctga cctaacctaa ccttaactcc ctgccagttc agcccatgcg gacccggcag
     7501 tcatgtcttt cgcttcagat tgaacgcggc tgcctcgtct cgatcaggat ttagcagaag
     7561 acagtacaca ctgaaaaagt accacaataa agaagctata ttgcaggcca aatagccgtc
     7621 tgctcggtct gtttattcaa cctggcctct ttcatcgttt ttcccttttc cttattaaac
     7681 catatatctc cagcagagcg atcgcgggag gagataattt agttgagatc gaagttggct
     7741 ttatagttgt aacagtgaaa tcagggcacg aatctttagt acttcgttga tcgccactcc
     7801 accaagggtt tgccattaag cataactggc aatatttttg tcaatttgcc ttttcgaccg
     7861 tgtaaatgtc cctatccata ctctccgcag tcaatatccg gaggtcaagt gtgaagactg
     7921 tttaccccag caagatactt ggacaagcct ggtttgcaat ccatagtttc agcaggcttc
     7981 ctaggtggcc ttaagcctgg gatactggct ggctgctagc aattgccatg actatgttgg
     8041 aggaaatgtt cgagaggaat cttgggtcca tgaacagagt agtcccactt caaatgttgc
     8101 gtatgcctga tattacggac caatagagga acaacctggc agcagctaac ttgtatatct
     8161 ctaatcgcgt tgacgaccgt tctgtgtcag tgcaactgcc acgcaaagac ttcagaataa
     8221 taaactttgc cagagaaggc agttgtatta tgttaatatc gcaacattgt gaaaattaaa
     8281 tgaactaaca atcaaaaaga aagaaaatcc gaactttccc tccgtattat ctaacccgtg
     8341 tgcttttgca acgcaatcca actattccag ttgacgacat taatccaagc cagcatagga
     8401 agaataccaa aaaccttcag acagccgtac ccgcaagcct tgttaccaac cagctatagc
     8461 aatcttaccc gttcaatttc ttaatcccca tctagacgct tccagcactc tgactgtcgg
     8521 gagacgggtt cctttgccag ccatgagcat tcgcctagtt agggagcggc ggatgcctac
     8581 ttacttctgg gaacgaggcg cgccgtgagc gggaaatatg ggtatcttgg gagaggagag
     8641 ttggagtagg gagaaatgct ggctcggtgg gaggacgagg gagctttaat aataccgctc
     8701 tggctttggt agcactgagc cagtggtcgc agcaaaagag gggaaaggag gggaacaggt
     8761 caagcggagc cgctcttgtt gaggatagct gttacccatg gaaattacat gtagcaagct
     8821 agcaacccgc cgaagtgccc cactggtctt caagagccag tccaggtgaa ctgcattccg
     8881 tccccttcgc cgcggtgccc actgcctcca cagcctagat gattctgaaa acacgttgaa
     8941 gagctcgcga taataaccga ggcgggtaat gcaggatgaa acttacgatc tacaacctac
     9001 agcaccctgg ttctgaaaca aagttgagga tccccgcgat cgataaccca gaccgtaatg
     9061 cgaattgcaa tttacgattt accccccgta gagcggactg attctggacc tatcttgaga
     9121 ctatgtggtg gagcctgcga tgataaccga ggccgtaatg caaaaatgaa aattacgaac
     9181 caagaggcgt gatgagaaga tcagctggat gactcccttt cgatgatacc ctttagcaga
     9241 agtgttctta aattgcggga agaggccgtt caggagtctg gctcgtttga tcacatggga
     9301 ttaaaatatg gtgttgaggc tgggatattt tatgttttgt ctcttgcgct tcgatatcaa
     9361 cagattgaca ataatgaata actatctacg gactatgaat gcagctcgat ctactccgta
     9421 tttagtcggg caatctcccg tagaagatgc tagactgcgc tgaaagacta ggcacagtta
     9481 attgccgcgg atcacgtcag gcctgacaaa tgacaggtgg accttcatgt aatcgcacga
     9541 tgagtcagcg cctcttctcc cccgccgact tgcagctgcg gatgtcccga ggagccgaac
     9601 attgaatcta tcccaagagt gactgaaata tgattgcaat cgtcgtcttt ttcttgcaga
     9661 gaatgtggtg aggtgatcaa tcgtgttttt cttcatatat tatattctgt aatgatcata
     9721 ctttgttgag atacccgtct ctaaaattgt actttcgcct ccctcctccc tctcaagtcc
     9781 gtcatcgcaa tgtcgaattt accgtcccat gcttctccgt cttttggggc gcaaacgcct
     9841 accacgccgc agcccgtcga cgagctttct ctcaagtcga acatcacatc agcccctgcg
     9901 ttacactcca gattagacga actccgagat gagacttcca gccagatcag cagcatagac
     9961 actgaccgcg gggacatgac ccctgcggtt cccgcatcat tactctcccc atccttcact
    10021 cctccagcaa cccccggggg ctcgatcaat accgcggagc tgcttcaaca gacccaatta
    10081 gctggcgcac ggcaagggtc aacacatact aagcccccga ggttgctttc tcgtcttccc
    10141 aatgtcgaat gtatcgtccg cgctcggata cccacgacaa ctggcgcgga gatgtttctt
    10201 cacctgtacc acaatgactt agacaacaaa gagcatttgg cgattgtgtt tggaaataca
    10261 attcgaagca gaagcttgga caagatccgg cctggagaga cggagatgga tcgcatgatc
    10321 cgtggcgcgt atgtcgggaa actccgaccg ggccgtgtga gcagctggta tgacgaggag
    10381 aaagctgaag aggccgccag tccggaagct ggcggcgctg tatccagtcc gcacacactg
    10441 ccgcgaagta gcctcaagga ggcacctctt gtgagaatcc attcggagtg ctacactggt
    10501 gagacggctt ggtctgcacg ctgcgactgc ggcgagcagc tcgatgaagc agctcgcctc
    10561 atgtcgctgc ctatggaaac gttggcagag gctgcgtcac cgccggatgg ggcggtaccg
    10621 tcaaatgctg ctggaggtgt aattgtgtac ctgcgccaag aaggacgagg cattgggctg
    10681 ggcgagaaac tgaaagcgta caatcttcag gatctgggat cagacacggt ggaagccaat
    10741 ctcctgcttc gccaccctgc ggatgcaaga agttatggat tagctacggc gatcctggtg
    10801 gatcttggtc tagggattga ctcgaatccg cacggcatcc gactactcac gaataacccg
    10861 gacaagattc gagcggttga gggacccaac cgagaagtgg tggtgaaaga gcgggtgccg
    10921 atggtgccat tggcatggag atcgggtgga aagatgggaa tcaagagttc cgaggtcgaa
    10981 gggtatctga gaacgaaggt atgtcgacaa tctatctctt gcaagctagc ttattttctc
    11041 atcattctta aacaatgtct cactctctcc tcgcagatat cgaaaatggg ccatttgctc
    11101 caatgatttc ctcttggagc aaaagtgtag tgccagtacg agtgttgtgg aggaaggctg
    11161 catacattgt gcctgtcatt aaacgatgag ctcgtccgta ttggcccctg taatgccatg
    11221 ttttccgccc ccaatcgtca aggttttccc tttgttagat tcctaccagt catctagcaa
    11281 gtgaggtaag ctttgccaga aacgccaagg ctttatctat gtagtcgata agcaaagtgg
    11341 actgatagct taatatggaa ggtccctcag gacaagtcga cctgtgcaga agagataaca
    11401 gcttggcatc acgcatcagt gcctcctctc agacagaata agtcgccata agttatcgac
    11461 cgaacctagg tagggtatat ttggtgacga caatacctcc cgacgatacc tggattagac
    11521 accgtatgcc gcgaattaac gccttccgac caagtccctt ttggtgatcg acactttagg
    11581 atcatctcct gccagtcgcc cagactgacg gtgttatggt ggcgcgctat ggctttccta
    11641 gccggtcaag ctccgtgcac tagagagcgg agaaggaggg ggagttgata gggaatgata
    11701 tctaggtaga gaagtactcc aaccagagat atgcggatca atgctccggc agttgttagc
    11761 ataccccaac ctcgtttaag tatcctcttc ttccatccat ctttatcatt atttgtctat
    11821 gtgcatccct agatcacgtc aattatttcg ataaatggat acgtgcactc gggaatgcgt
    11881 gattggctcg tctcgtgttc tcagttcctc attccccccc acgttacatt gacaacggag
    11941 tattagccta tggtcctgtt cgataagctt gctccatcgc ctccactacg tcaagcggag
    12001 tatcagacat gagctctacc ccagatatcg cctgcttatc gctggttgct ttgtcatgca
    12061 agtacagatc acatcggcaa ttctgataac agatggtgcc attgtcccgt aatcaattgc
    12121 ccattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    12181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    12241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    12301 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    12361 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    12421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    12481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    12541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    12601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    12661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    12721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    12781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    12841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    12901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    12961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    13021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    13081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    13141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    13201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    13261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    13321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    13381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    13441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    13501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    13561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    13801 ctggcctttt gctcatgatt acgaattcat cacgtgctat aaaaataatt ataatttaaa
    13861 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
    13921 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
    13981 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
    14041 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
    14101 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
    14161 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
    14221 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
    14281 ttattccatc attaaaagat acgaggcgcg tgtaagttac gaattcgagc tcggtacccg
    14341 ggataacttc gtataatgta tgctatacga agttatgggt aataactgat ataattaaat
    14401 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
    14461 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
    14521 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
    14581 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
    14641 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
    14701 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
    14761 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
    14821 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
    14881 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
    14941 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
    15001 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
    15061 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
    15121 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
    15181 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
    15241 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
    15301 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
    15361 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
    15421 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
    15481 agcgtggtgc a
//
