LOCUS       sequence-35021-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: MKK4
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgagaacct gtacttccaa tccaatgcgg ctccgagccc ggatccggca gccaacatgg
       61 actataagga cgatgacgac aaacagggta aacgcaaagc actgaagttg aattttgcaa
      121 atccaccttt caaatctaca gcaaggttta ctctgaatcc caatcctaca ggagttcaaa
      181 acccacacat agagagactg agaacacaca gcattgagtc atcaggaaaa ctgaagatct
      241 cccctgaaca acactgggat ttcactgcag aggacttgaa agaccttgga gaaattggac
      301 gaggagctta tggttctgtc aacaaaatgg tccacaaacc aagtgggcaa ataatggcag
      361 ttaaaagaat tcggtcaaca gtggatgaaa aagaacaaaa acaacttctt atggatttgg
      421 atgtagtaat gcggagtagt gattgcccat acattgttca gttttatggt gcactcttca
      481 gagagggtga ctgttggatc tgtatggaac tcatgtctac ctcgtttgat aagttttaca
      541 aatatgtata tagtgtatta gatgatgtta ttccagaaga aattttaggc aaaatcactt
      601 tagcaactgt gaaagcacta aaccacttaa aagaaaactt gaaaattatt cacagagata
      661 tcaaaccttc caatattctt ctggacagaa gtggaaatat taagctctgt gacttcggca
      721 tcagtggaca gcttgtggac tctattgcca agacaagaga tgctggctgt aggccataca
      781 tggcacctga aagaatagac ccaagcgcat cacgacaagg atatgatgtc cgctctgatg
      841 tctggagttt ggggatcaca ttgtatgagt tggccacagg ccgatttcc
//
