LOCUS       sequence-350214-       12380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-GUS driven by the bZIP9 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Kim JY, Symeonidi E, Pang TY, Denyer T, Weidauer D, Bezrutczyk M, 
            Miras M, Zollner N, Hartwig T, Wudick MM, Lercher M, Chen LQ, 
            Timmermans MCP, Frommer WB
  TITLE     Distinct identities of leaf phloem cells revealed by single cell 
            transcriptomics.
  JOURNAL   Plant Cell. 2021 May 5;33(3):511-530. doi: 10.1093/plcell/koaa060.
  PUBMED    33955487
REFERENCE   2  (bases 1 to 12380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Cell"; date: "2021-05-5- 5"; volume: "33"; issue: "3"; pages:
            "511-530"
COMMENT     Sequence Label: pbZIP9:GFP-GUS
FEATURES             Location/Qualifiers
     source          1..12380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..265
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(358..610)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(630..1181)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1223..1402)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    1586..1610
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3168..3192)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3205..3207,3208..3210,3211..3918)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(3205..3207,3208..3210,3211..3918)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3250..3271)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3511..3530)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3858..3879
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3928..5739
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADFRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     regulatory      5740..5749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6059..6083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7414..8043
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8472..9545
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9611..9805
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10041..10063)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10149..10289
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10204..10223
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10304..10321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10475..11063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10555..10574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11309..12100)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
       61 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
      121 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
      181 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
      241 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
      301 gacgttttta atgtactgaa ttaacgccga attgaattat cagcttgcat gccggtcgat
      361 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
      421 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
      481 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
      541 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
      601 ttgaacgatc tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg
      661 ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc
      721 ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc
      781 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc
      841 agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg
      901 gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc
      961 gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc
     1021 cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg
     1081 accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg
     1141 gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga
     1201 gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     1261 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     1321 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt
     1381 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     1441 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa ttcgagctcg
     1501 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gctctcccat atggtcgacc
     1561 tgcaggcggc cgcactagtg atatcacaag tttgtacaaa aaagcaggct tccgtttgga
     1621 gattacaagt agagtttgta gaaacagaaa aattacttct tctttttttg ctaaaagaaa
     1681 acaaaaatta cttttgtgtt cataaactaa ttttcattca tattttctgt attttaactt
     1741 acaaatttag catgtgcctc aagtagaaat tcgtttctta caaaaaacgt atatcaatca
     1801 tcatctaaat tacatgaaaa ttttcaacat cactaaggat tgaattttaa ttgtactagt
     1861 tactatggtt actgaattgt actgtagtcg gtagattgta aacattgtag tccactatag
     1921 gttcgaaagt tctagttagt gagaaataat aattttaaaa tgcttttgga ttctattttc
     1981 ggatctaaac aacttttgtt tgtttaaaga cacattacct agtttgtttc tttttattgt
     2041 tttgttgtga aatattttaa ctttttaaag gaagtgcaga tcaaaagaaa gaaaagttgg
     2101 tttacgcata caaaagttga tgaaataaaa agtatttctg gtgctggagg aaagaaatgt
     2161 cttttagatc tgctttccaa aagcatatgg cgaaaagtaa gagtaaacaa aggacgaagc
     2221 attttgtgtt gttcgtgtga ccggaaaatt cttaagtttt tctttttggt gttgtagtag
     2281 taatgaatga taataaatat tttaaagatt tttactacat gatacaaagg tgtaaaaaac
     2341 tgaaacaata ttatcaatat ttttccttca aatattatca attatttatg aagattagtt
     2401 tttagtgttt tatgaaatta tatattcggt aaaacattta ccgtaaaagg ttaatttatt
     2461 cattttcaat gtttttgtag tttcatcatt ggactatgcc ccttgtatat taatcttaaa
     2521 ttagtcgtta agtacttcaa attcgaattc aaacaggtta tgtagtagag taatttatgt
     2581 aagtgtaagg ttcttgacat gtgccaagaa tacaaatgta catgagtgac aatattatta
     2641 tctaaatatt ttcaaatatt taaaagctta tttcgaaata ttttaggatc cttttatgaa
     2701 tataagctgc ttatatatat gcctataaca ttcattttca aatcaaaagc tccaacatat
     2761 taatgagctg acaaacaact taatgtttga attcagatat gatttatatt gaaagagtct
     2821 ccagctatct tagatagtct ccctagttaa gggattaaag gttatcatta gcatataatt
     2881 ggtaccataa tattcaccaa tactacagag cctaatatac atctctgtgg ctttgctttt
     2941 ctattctctt ttgttattgt tgtcattaaa gaaaaaaaaa gttggaaatg aggacgttgc
     3001 attgacaatg caaattcaat tttgcttagc cattaagaag ctttttcaca actttagtaa
     3061 agaagagaga cttatataaa gaagatgtct atcaccactt actcttcccc tctcctctct
     3121 tttccaacca attctcttct ttcttgtgtg ttcacattca aagaaatacc cagctttctt
     3181 gtacaaagtg gtgatatccc gcggatggtg agcaagggcg aggagctgtt caccggggtg
     3241 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3301 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3361 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3421 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3481 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3541 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3601 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3661 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3721 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3781 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3841 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3901 ggcatggacg agctgtacga acccggcatg ttacgtcctg tagaaacccc aacccgtgaa
     3961 atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgat
     4021 cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt
     4081 aacgatcagt tcgccgatgc agattttcgt aattatgcgg gcaacgtctg gtatcagcgc
     4141 gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc
     4201 actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca gggcggctat
     4261 acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcacc
     4321 gtttgcgtga acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac
     4381 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat
     4441 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
     4501 catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat
     4561 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc
     4621 gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa
     4681 ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc
     4741 cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact
     4801 ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg
     4861 gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac
     4921 ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa
     4981 actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg
     5041 aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg
     5101 attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc
     5161 aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca
     5221 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
     5281 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggatgg
     5341 tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga acttctggcc
     5401 tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga tacgttagcc
     5461 gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat
     5521 atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt atggaatttc
     5581 gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc
     5641 actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gactggcatg
     5701 aacttcggtg aaaaaccgca gcagggaggc aaacaatgac catggcggcc gggagggcca
     5761 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     5821 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     5881 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     5941 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgagc
     6001 ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     6061 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat
     6121 ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg
     6181 gttcccctcg ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc
     6241 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     6301 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     6361 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     6421 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     6481 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     6541 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     6601 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     6661 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     6721 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     6781 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     6841 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     6901 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     6961 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     7021 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     7081 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     7141 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     7201 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     7261 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     7321 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     7381 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     7441 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     7501 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     7561 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     7621 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     7681 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     7741 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     7801 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     7861 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     7921 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     7981 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     8041 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     8101 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     8161 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     8221 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     8281 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     8341 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     8401 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     8461 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     8521 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     8581 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     8641 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     8701 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     8761 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     8821 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     8881 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     8941 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     9001 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     9061 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     9121 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     9181 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     9241 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     9301 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     9361 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     9421 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     9481 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     9541 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     9601 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     9661 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     9721 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     9781 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     9841 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     9901 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     9961 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    10021 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
    10081 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    10141 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    10201 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10261 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    10321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    10921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11101 agtggaacga aaactcacgt taagggattt tggtcatgca tgatatatct cccaatttgt
    11161 gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct
    11221 gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg
    11281 tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt
    11341 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
    11401 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
    11461 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
    11521 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
    11581 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
    11641 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
    11701 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
    11761 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
    11821 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
    11881 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
    11941 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
    12001 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
    12061 atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag
    12121 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
    12181 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat
    12241 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
    12301 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg
    12361 tccactgggt tcgtgcccga
//
