LOCUS       sequence-35022-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: MKK4
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..733
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     691..713
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             761..781
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctga tgtcccctat actaggttat tggaaaatta agggccttgt
      121 gcaacccact cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga
      181 gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa
      241 tcttccttat tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta
      301 tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat
      361 gcttgaagga gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga
      421 ctttgaaact ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga
      481 agatcgttta tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat
      541 gttgtatgac gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc
      601 aaaattagtt tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa
      661 atccagcaag tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga
      721 ccatcctcca aaatcggatg attacccaga tctgggtacc gagaacctgt acttccaatc
      781 caatgcggct ccgagcccgg atccggcagc caacatggac tataaggacg atgacgacna
      841 acagggtaaa cgcaaagcac tgaagttgaa ttttgcaaat cca
//
