LOCUS       sequence-350223-        4855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCerulean3 fused to the N-terminus of the Bcl-2 family 
            protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to 
            make stable BMK-DKO cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Liu Q, Osterlund EJ, Chi X, Pogmore J, Leber B, Andrews DW
  TITLE     Bim escapes displacement by BH3-mimetic anti-cancer drugs by 
            double-bolt locking both Bcl-XL and Bcl-2.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 37689. doi: 10.7554/eLife.37689.
  PUBMED    30860026
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 37689. doi"; pages: " 
            10.7554/eLife.37689"
COMMENT     Sequence Label: mCerulean3-Bcl-XL-s2193
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..330
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(162..182)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      1469..1478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1475..1477,1478..1480,1481..2191)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1520..1541)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1781..1800)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2128..2149
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2968..3412
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        3446..3492
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3484..3502
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3976..4097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3987..4006)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4041..4060
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      join(4285..4855,1..17)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4773..4792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
       61 ggccttttgc tcacatgttc ttaattaacc tgcagggcct gaaataacct ctgaaagagg
      121 aacttggtta ggtaccttct gaggctgaaa gaaccagctg tggaatgtgt gtcagttagg
      181 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      241 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      301 gcatctcaat tagtcagcaa ccatagtccc actagttccg ccagagcgcg cgagggcctc
      361 cagcggccgc ccctccccca cagcaggggc ggggtcccgc gcccaccgga aggagcgggc
      421 tcggggcggg cggcgctgat tggccggggc gggcctgacg ccgacgcggc tataagagac
      481 cacaagcgac ccgcagggcc agacgttctt cgccgaagct tgccgtcaga acgcaggtga
      541 ggggcgggtg tggcttccgc gggccgccga gctggaggtc ctgctccgag cgggccgggc
      601 cccgctgtcg tcggcgggga ttagctgcga gcattcccgc ttcgagttgc gggcggcgcg
      661 ggaggcagag tgcgaggcct agcggcaacc ccgtagcctc gcctcgtgtc cggcttgagg
      721 cctagcgtgg tgtccgcgcc gccgccgcgt gctactccgg ccgcactctg gtcttttttt
      781 tttttgttgt tgttgccctg ctgccttcga ttgccgttca gcaatagggg ctaacaaagg
      841 gagggtgcgg ggcttgctcg cccggagccc ggagaggtca tggttgggga ggaatggagg
      901 gacaggagtg gcggctgggg cccgcccgcc ttcggagcac atgtccgacg ccacctggat
      961 ggggcgaggc ctggggtttt tcccgaagca accaggctgg ggttagcgtg ccgaggccat
     1021 gtggccccag cacccggcac gatctggctt ggcggcgccg cgttgccctg cctccctaac
     1081 tagggtgagg ccatcccgtc cggcaccagt tgcgtgcgtg gaaagatggc cgctcccggg
     1141 ccctgttgca aggagctcaa aatggaggac gcggcagccc ggtggagcgg gcgggtgagt
     1201 cacccacaca aaggaagagg gcctggtccc tcaccggctg ctgcttcctg tgaccccgtg
     1261 gtcctatcgg ccgcaatagt cacctcgggc ttttgagcac ggctagtcgc ggcgggggga
     1321 ggggatgtaa tggcgttgga gtttgttcac atttggtggg tggagactag tcaggccagc
     1381 ctggcgctgg aagtcatttt tggaatttgt ccccttgagt tttgagcgga gctaattcaa
     1441 agcaaccggt gatctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
     1501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
     1681 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatccacgg
     1921 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcggcctcaa
     1981 ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     2101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2161 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag aaatgagcca
     2221 aagcaaccgg gagctggtgg ttgactttct ctcctacaag ctttcccaga aaggatacag
     2281 ctggagtcag tttagtgatg tggaagagaa caggactgag gccccagaag ggactgaatc
     2341 ggagatggag acccccagtg ccatcaatgg caacccatcc tggcacctgg cagacagccc
     2401 cgcggtgaat ggagccactg gccacagcag cagtttggat gcccgggagg tgatccccat
     2461 ggcagcagta aagcaagcgc tgagggaggc aggcgacgag tttgaactgc ggtaccggcg
     2521 ggcattcagt gacctgacat cccagctcca catcacccca gggacagcat atcagagctt
     2581 tgaacaggta gtgaatgaac tcttccggga tggggtaaac tggggtcgca ttgtggcctt
     2641 tttctccttc ggcggggcac tgtgcgtgga aagcgtagac aaggagatgc aggtattggt
     2701 gagtcggatc gcagcttgga tggccactta cctgaatgac cacctagagc cttggatcca
     2761 ggagaacggc ggctgggata cttttgtgga actctatggg aacaatgcag cagccgagag
     2821 ccgaaagggc caggaacgct tcaaccgctg gttcctgacg ggcatgactg tggccggcgt
     2881 ggttctgctg ggctcactct tcagtcggaa atgaagatcc accggatcta gataactgat
     2941 ccacgcgtat cgattgtcga ccctaggagc aggtttcccc aatgacacaa aacgtgcaac
     3001 ttgaaactcc gcctggtctt tccaggtcta gaggggtaac actttgtact gcgtttggct
     3061 ccacgctcga tccactggcg agtgttagta acagcactgt tgcttcgtag cggagcatga
     3121 cggccgtggg aactcctcct tggtaacaag gacccacggg gccaaaagcc acgcccacac
     3181 gggcccgtca tgtgtgcaac cccagcacgg cgactttact gcgaaaccca ctttaaagtg
     3241 acattgaaac tggtacccac acactggtga caggctaagg atgcccttca ggtaccccga
     3301 ggtaacacgc gacactcggg atctgagaag gggactgggg cttctataaa agcgctcggt
     3361 ttaaaaagct tctatgcctg aataggtgac cggaggtcgg cacctttcct ttgcaattac
     3421 tgaccctatg aatacaactg actgtttgac aattaatcat cggcatagta tatcggcata
     3481 gtataatacg actcactata ggagggccac catgaagacc ttcaacatct ctcagcagga
     3541 tctggagctg gtggaggtcg ccactgagaa gatcaccatg ctctatgagg acaacaagca
     3601 ccatgtcggg gcggccatca ggaccaagac tggggagatc atctctgctg tccacattga
     3661 ggcctacatt ggcagggtca ctgtctgtgc tgaagccatt gccattgggt ctgctgtgag
     3721 caacgggcag aaggactttg acaccattgt ggctgtcagg cacccctact ctgatgaggt
     3781 ggacagatcc atcagggtgg tcagcccctg tggcatgtgc agagagctca tctctgacta
     3841 tgctcctgac tgctttgtgc tcattgagat gaatggcaag ctggtcaaaa ccaccattga
     3901 ggaactcatc cccctcaagt acaccaggaa ctaaacctga attcttcgaa ctcgaggcta
     3961 gctggccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4021 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4081 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4141 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gaaatgttaa
     4201 ttaactagcc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc agggg
//
