LOCUS       sequence-350232-        9974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Functions as a genetic Cre reporter that switches from DsRed 
            expression to eGFP expression upon presence of Cre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E, 
            Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T, 
            Pegtel DM, van Rheenen J
  TITLE     In Vivo imaging reveals extracellular vesicle-mediated phenocopying 
            of metastatic behavior.
  JOURNAL   Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042.
  PUBMED    26000481
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.04"; journalName: "Cell"; date: "2015-05-21- 
            21"; volume: "161"; issue: "5"; pages: "1046-57"
COMMENT     Sequence Label: pLV-CMV-LoxP-DsRed-LoxP-eGFP
FEATURES             Location/Qualifiers
     source          1..9974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(662..695)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      724..733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             730..1407
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(910..930)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1128..1147
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1322..1345
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(1432..1465)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1516..1525
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1522..1524,1525..1527,1528..2241)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1522..1524,1525..1527,1528..2238)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1567..1588)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1828..1847)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2175..2196
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2487..3039
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2631..2648)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2858..2877
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3066..3665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3066..3085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3562..3582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4557..4790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4862..4983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4899..4918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4953..4972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5023..5158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5178..5197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5179..5197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5207..5224)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5207..5223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5216..5238)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5365..5820
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5452..5471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5662..5683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5846..5950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5951..6019,6020..6811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6169..6188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6982..7570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7471..7490
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7724..7741
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7858..7879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7894..7899,7900..7917,7918..7924)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7932..7948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7937..7959
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7956..7972
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7956..7972
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7991..8011
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7993..8011
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8039..8265
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8266..8446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8493..8618
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9111..9344
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9529..9573
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9835..9952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagctagagg atcccccggg
      601 ctgcaggaat tcggcgcgcc acgcgtaccg atagcttcga attctgcagt cgaccggaat
      661 tataacttcg tatagcatac attatacgaa gttataattc tgcagatatc cagcacagtg
      721 gcggccacca tggatagcac tgagaacgtc atcaagccct tcatgcgctt caaggtgcac
      781 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggcaagccc
      841 tacgagggca cccagaccgc caagctgcag gtgaccaagg gcggccccct gcccttcgcc
      901 tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc
      961 gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1021 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcacc
     1081 ttcatctacc acgtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag
     1141 aagaagactc tgggctggga gccctccacc gagcgcctgt acccccgcga cggcgtgctg
     1201 aagggcgaga tccacaaggc gctgaagctg aagggcggcg gccactacct ggtggagttc
     1261 aagtcaatct acatggccaa gaagcccgtg aagctgcccg gctactacta cgtggactcc
     1321 aagctggaca tcacctccca caacgaggac tacaccgtgg tggagcagta cgagcgcgcc
     1381 gaggcccgcc accacctgtt ccagtagagc tagctgaata aggccgctcg aataacttcg
     1441 tatagcatac attatacgaa gttattcgag tctagagggc ccgttagatc tctcgaggtt
     1501 aacgaattcg actacgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1561 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1621 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1681 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1741 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1801 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1861 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1921 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1981 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2041 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2101 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2161 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2221 atggacgagc tgtacaagta aaattctacc gggtagcgac tagtctcgag tctagacagc
     2281 gctcaccggt cgttaacatt aagctagcct agtgccattt gttcagtggt tcgtagggct
     2341 ttcccccact gtttggcttt cagttatatg gatgatgtgg tattgggggc caagtctgta
     2401 cagcatcttg agtccctttt taccgctgtt accaattttc ttttgtcttt gggtaaaccc
     2461 gggaattccg cccctctccc cccctaacgt tactggccga agccgcttgg aataaggccg
     2521 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     2581 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     2641 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     2701 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     2761 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     2821 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     2881 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     2941 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     3001 cggggacgtg gttttccttt gaaaaacacg atgataatat ggatatggcc acaacggtcg
     3061 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     3121 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     3181 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     3241 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     3301 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     3361 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3421 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3481 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     3541 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     3601 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     3661 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     3721 gtattcaatc taagcaggct ttcactttct cgccaactta caaggccttt ctgtgtaaac
     3781 aatacctgaa cctttacccc gttgcccggc aacggccagg tctgtgccaa gtgtttgctg
     3841 acgcaacccc cactggctgg ggcttggtca tgggccatca gcgcatgcgt ggaacctttt
     3901 cggctcctct gccgatccat actgcggaac tcctagccgc ttgttttgct cgcagcaggt
     3961 ctggagcaaa cattatcggg actgataact ctgttgtcct atcccgcaaa tatacatcgt
     4021 ttccatggct gctaggctgt gctgccaact ggatcctgcg cgggacgtcc tttgtttacg
     4081 tcccgtcggc gctgaatcct gcggacgacc cttctcgggg tcgcttggga ctctctcgtc
     4141 cccttctccg tctgccgttc cgaccgacca cggggcgcac ctctctttac gcggactccc
     4201 cgtctgtgcc ttctcatctg ccggaccgtg tgcacttcgc ttcacctctg cacgtcgcat
     4261 ggagaccacc gtgaacgccc accaaatatt gcccaaggtc ttacataaga ggactcttgg
     4321 actctcagca atgtcaacga ccgaccttga ggcatacttc aaagactgtt tgtttaaaga
     4381 ctgggaggag ttgggggagg agattaggtt aaaggtcttt gtactaggag gctgtaggca
     4441 taaattggtc tgcgcaccag caccatggcg caatcactag agcggggtac ctttaagacc
     4501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4561 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4621 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4981 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5101 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5161 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     5221 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     5281 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     5341 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5761 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5821 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5881 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5941 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6001 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6061 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6121 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6181 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6241 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6301 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6361 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6421 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6481 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6541 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6601 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6661 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6721 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6781 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6841 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6901 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6961 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7021 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7081 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7141 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7201 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7261 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7321 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7381 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7441 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7501 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7561 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7621 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7681 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7741 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7801 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7861 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7921 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7981 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     8041 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     8101 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     8161 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     8221 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     8281 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8341 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8401 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     8461 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8521 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     8581 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     8641 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     8701 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     8761 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     8821 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     8881 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8941 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9001 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9061 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9121 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9181 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9241 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9301 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     9361 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     9421 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9481 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9541 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9601 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     9661 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     9721 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     9781 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttttaa
     9841 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9901 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgataa
     9961 gcttgggagt tccg
//
