LOCUS       sequence-35025-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: MKK2
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..743
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     701..723
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             771..791
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgcgaattcc ctctgaatat tttgtttaac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctga tgtcccctat actaggttat tggaaaatta
      121 agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa tatgaagagc
      181 atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg
      241 agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag tctatggcca
      301 tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag
      361 agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg agaattgcat
      421 atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct gaaatgctga
      481 aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat gtaacccatc
      541 ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca atgtgcctgg
      601 atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca caaattgata
      661 agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa gccacgtttg
      721 gtggtggcga ccatcctcca aaatcggatg attacccaga tctgggtacc gagaacctgt
      781 acttccaatc caatgcgctg gcccggagga agccggtgct gccggcgctc accatcaacc
      841 ctaccatcgc cgagggccca tcccctacca gcgagggagc atccgaggca aacctggtgg
      901 acctgcagaa gaagctggag gagctggaac ttgacgcctt tctcacccag aaagccaagg
      961 ttggcgaact
//
