LOCUS       sequence-35028-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Map3k1
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..728
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     686..708
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaatattttg tttactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgatgtcc cctatactag gttattggaa aattaagggc cttgtgcaac
      121 ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg
      181 atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc
      241 cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag
      301 ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg
      361 aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg
      421 aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc
      481 gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt
      541 atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat
      601 tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca
      661 gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc
      721 ctccaaaatc ggatgattac ccagatctgg gtaccgagaa cctgtacttc cnatccaatg
      781 atgggcatca ggacagctta caggcgctgg cccccgccag ctgtctagaa aacagctccc
      841 cttgagcaca cagtccatag agagaaaact ggaaaaggac taagtgctac gagactgagt
      901 gccagctcgg aggacatttc ctgacaga
//
