LOCUS       sequence-350324-        2864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone. T7Max promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Deich C, Cash B, Sato W, Sharon J, Aufdembrink L, Gaut NJ, Heili J, 
            Stokes K, Engelhart AE, Adamala KP
  TITLE     T7Max transcription system.
  JOURNAL   J Biol Eng. 2023 Jan 23;17(1):4. doi: 10.1186/s13036-023-00323-1.
  PUBMED    36691081
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2023-01-23"; volume: "17(1):4. doi"; pages: " 
            10.1186/s13036-023-00323-1"
COMMENT     Sequence Label: pCI-T7Max-UTR1-T500
FEATURES             Location/Qualifiers
     source          1..2864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      310..765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(397..416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     607..628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(832..851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     951..973
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1011..1029)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1097..1201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1202..1270,1271..2062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1420..1439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2233..2821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2722..2741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatggct cgacagatct aattctaata cgactcacta tagggaaata
       61 attttgttta actttaagaa ggagatatac cggtctcgag caaagcccgc cgaaaggcgg
      121 gcttttctgt acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
      181 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgggc
      241 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      301 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
      721 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
      781 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      841 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      901 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      961 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     1021 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1201 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1261 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1321 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1381 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1441 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1501 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1561 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1621 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1681 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1741 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1801 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1861 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1921 gcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1981 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2041 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2101 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2341 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgct
//
