LOCUS       sequence-350377-       13522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast test vector of pPPED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read 
            AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ
  TITLE     Spelling Changes and Fluorescent Tagging With Prime Editing Vectors 
            for Plants.
  JOURNAL   Front Genome Ed. 2021 Mar 4;3:617553. doi: 
            10.3389/fgeed.2021.617553. eCollection 2021.
  PUBMED    34713247
REFERENCE   2  (bases 1 to 13522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-03-4"; volume: "3:617553. doi"; pages: " 
            10.3389/fgeed.2021.617553. eCollection 2021"
COMMENT     Sequence Label: pPPEDs
FEATURES             Location/Qualifiers
     source          1..13522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     671..687
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        902..1578
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1515..1535
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1630..1650
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1657..4170,4171..4173,4174..5757)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7945..7965
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8032..8284
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8325..8341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8540..8664
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8701..8706,8707..8724,8725..8731)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8785..9444
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8851..8870)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9786..10091
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9970..9989
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10132..10256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    10283..10307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        complement(11337..11355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11376..11392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11376..11392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11389..11411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11400..11416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11424..11430,11431..11448,11449..11454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11469..11490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11607..11624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11778..12366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11858..11877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12537..13328,13329..13397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13160..13179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13398..13502)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtaccg
      661 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg
      721 gatccactag tgagtcgtat tacaattcat gactctctta aggtagccaa aatcgatatc
      781 tagggataac agggtaatcg atcactagaa ttcggcgcgc cgggcccaac atggtggagc
      841 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
      901 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      961 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
     1021 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
     1081 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     1141 tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat
     1201 acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac
     1261 ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa
     1321 ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct
     1381 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
     1441 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
     1501 gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat
     1561 ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct ctcgaccgct
     1621 agcatggcac ctaagaaaaa gaggaaggtt ggtattgata agaagtactc tatcggactc
     1681 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct
     1741 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt
     1801 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga
     1861 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag
     1921 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa
     1981 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac
     2041 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct
     2101 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc
     2161 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg
     2221 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag
     2281 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc
     2341 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc
     2401 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat
     2461 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg
     2521 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac
     2581 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac
     2641 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag
     2701 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa
     2761 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc
     2821 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct
     2881 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc
     2941 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct
     3001 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct
     3061 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag
     3121 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct
     3181 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac
     3241 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt
     3301 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc
     3361 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca
     3421 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac
     3481 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga
     3541 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag
     3601 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga
     3661 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct
     3721 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag
     3781 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga
     3841 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag
     3901 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc
     3961 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa
     4021 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag
     4081 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat
     4141 atcaacaggc tctctgatta cgatgttgat gctatcgtgc cacagtcatt cttgaaggat
     4201 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac
     4261 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct
     4321 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct
     4381 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag
     4441 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg
     4501 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat
     4561 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt
     4621 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg
     4681 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc
     4741 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag
     4801 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca
     4861 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg
     4921 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct
     4981 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag
     5041 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt
     5101 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg
     5161 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag
     5221 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt
     5281 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca
     5341 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca
     5401 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc
     5461 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag
     5521 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc
     5581 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca
     5641 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat
     5701 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgatctc
     5761 agtggtggga gcagtggtgg atcatcaggg agtgagactc caggtacaag tgaaagcgct
     5821 actcctgaat cttcaggagg ttcctcagga ggaagttcta ctcttaacat cgaggacgag
     5881 tatcgtctcc acgagaccag caaggaacct gatgtatctc taggatcaac ttggctcagt
     5941 gatttccccc aggcatgggc agaaacaggt ggcatgggtt tggctgtacg tcaagcacca
     6001 cttataattc ctctaaaagc aacaagcacc cccgtcagta ttaagcagta cccaatgagt
     6061 caggaggcta gattgggcat taagccacac atacagagac ttttagatca aggcattctg
     6121 gtaccctgtc agtccccctg gaacacacca ctgctgcctg ttaagaaacc aggaaccaac
     6181 gattaccgac cagttcagga cttgcgagaa gtgaacaaaa gggtcgagga tatccaccct
     6241 accgtgccta atccatacaa ccttttatct ggccttcctc caagccatca atggtacacc
     6301 gttctagatt tgaaagatgc attcttttgt ctgcgactcc accccacctc acagcccctt
     6361 ttcgccttcg agtggagaga ccctgaaatg ggtatcagcg gccaacttac ttggactagg
     6421 ttaccccagg gatttaaaaa cagtccaacc ctattcaatg aggcattgca cagggacctg
     6481 gcagattttc gtatacaaca ccccgatcta atattgttac aatacgtgga cgaccttctc
     6541 ctagctgcaa caagcgagtt agattgtcag caaggaacaa gggctctttt gcaaacacta
     6601 ggaaacttgg ggtatcgtgc tagtgccaaa aaagctcaga tttgtcaaaa gcaggtaaag
     6661 tacttagggt atttactaaa ggaaggtcag agatggttaa ccgaagcccg aaaggaaact
     6721 gtaatggggc aaccaactcc taaaactccc agacaactca gggagttctt gggcaaagca
     6781 gggttttgta gacttttcat acccggcttt gctgaaatgg cagcacccct gtatcccttg
     6841 acaaagcctg ggacactatt taactggggg ccagaccagc agaaggcata ccaagaaata
     6901 aagcaagcac tactcaccgc acctgcccta ggcctgcctg atctgactaa accatttgaa
     6961 ctcttcgtag atgagaaaca aggctacgct aaaggtgtgc tgacacaaaa attgggaccc
     7021 tggcgacgac cagtcgccta cctcagcaag aaattagacc ccgtggccgc cggttggcca
     7081 ccatgcttac gaatggtggc agcaatcgcc gtcctgacaa aggatgcagg caaacttacc
     7141 atgggccaac ctctagtcat cttagcaccc catgccgttg aggctctcgt aaagcagcca
     7201 cctgatagat ggctcagtaa cgctcgaatg acccactatc aagccctcct cttggatacc
     7261 gacagagtcc agttcggacc cgtagtagca cttaacccag caacactgct acccctccct
     7321 gaggaagggc tacaacataa ctgcctagac attttggccg aggcacatgg gacacgtcca
     7381 gacttgacag atcagccctt accagacgcc gaccacacat ggtacacaga cggaagttca
     7441 ctcttgcagg agggtcagag gaaggccggg gctgctgtga ctaccgagac cgaagttatt
     7501 tgggctaagg ccctaccagc cggcacctct gcacagagag cagagttaat tgcactaacc
     7561 caggccctca aaatggccga aggtaaaaaa ctaaatgtct atacagattc acgatatgct
     7621 tttgcaacag cccatataca tggcgaaatt tataggagga gggggtggtt aacatcagag
     7681 ggaaaagaga taaaaaacaa ggacgaaatc cttgcattgc taaaggcttt attccttccc
     7741 aagcgtttaa gcataatcca ctgtcccggt catcagaagg gtcactccgc cgaggcaaga
     7801 ggcaaccgaa tggctgacca agccgcaagg aaggccgcaa tcactgagac cccagacacc
     7861 tctactctac taatcgaaaa cagctcccct tcaggtggaa gcaagagaac cgccgacggc
     7921 agtgaattcg aaagcagggc tgaccccaag aagaagagga aggtgtgacc gcggatatct
     7981 gcagaagctt acgcgtcgac gtctcgaggc cggccgagct cgaatttccc cgatcgttca
     8041 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     8101 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     8161 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     8221 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     8281 gatcgggaat tgatatctag ggataacagg gtaataagct tggcactggc cgtcgtttta
     8341 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8401 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8461 cgcagcctga atggcgaatg agcttgagct tggatcagat tgtcgtttcc cgccttcagt
     8521 ttggactcta gagttatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     8581 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     8641 aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg caccccaggc
     8701 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     8761 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     8821 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     8881 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     8941 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     9001 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     9061 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     9121 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     9181 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     9241 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     9301 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     9361 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     9421 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     9481 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     9541 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     9601 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     9661 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     9721 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     9781 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     9841 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     9901 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     9961 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
    10021 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
    10081 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
    10141 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
    10201 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
    10261 aacagcggaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    10321 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    10381 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtacttta aagtacttta
    10441 aagtacttta aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
    10501 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
    10561 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
    10621 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
    10681 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
    10741 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
    10801 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
    10861 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
    10921 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
    10981 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
    11041 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
    11101 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
    11161 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
    11221 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
    11281 tgaccgaggc cgacgccctg gcggccgcca ccgcggtgga gctccagctt ttgttccctt
    11341 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    11401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    11461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    11521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    11581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    11641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    11701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    11761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    11821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    11881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    11941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    12121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    12181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    12241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    12301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    12361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    12421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    12481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    12541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    12601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    12661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    12721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    12781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    12841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    12901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    12961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    13201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    13261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    13321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    13381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    13441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    13501 cgcacatttc cccgaaaagt gc
//
