LOCUS       sequence-350393-        8458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STREAMING-tag cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, 
            Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
  TITLE     STREAMING-tag system reveals spatiotemporal relationships between 
            transcriptional regulatory factors and transcriptional activity.
  JOURNAL   Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2.
  PUBMED    36539402
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-20"; volume: "13(1):7672. doi"; pages: " 
            10.1038/s41467-022-35286-2"
COMMENT     Sequence Label: 
            pBSK-Delta-BS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-
            NeoR-GS4
FEATURES             Location/Qualifiers
     source          1..8458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(47..137)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(176..631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(313..334)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     525..544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     758..780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     772..789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     773..789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     799..818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        799..817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1312..1321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1624..1642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1682..1700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1727..1745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1756..1774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1785..1803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1830..1848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1859..1877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1888..1906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1933..1951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1962..1980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1991..2009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2065..2083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2094..2112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2139..2157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2168..2186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2197..2215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2242..2260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2271..2289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2300..2318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2374..2392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2448..2466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2506..2524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2551..2569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2580..2598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2609..2627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2654..2672
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2683..2701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2815..2833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2860..2878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2889..2907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2963..2981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2992..3010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3021..3039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3066..3084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3095..3113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3124..3142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3169..3187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3227..3245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3272..3290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3301..3319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3330..3348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3375..3393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3404..3422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3433..3451
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3478..3496
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3507..3525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3536..3554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3581..3599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3610..3628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3639..3657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3684..3702
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3713..3731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3742..3760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3787..3805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3816..3834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3845..3863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3890..3908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3919..3937
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3948..3966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3993..4011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4022..4040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4051..4069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4096..4114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4154..4172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4199..4217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4228..4246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4257..4275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4302..4320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4331..4349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4360..4378
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4405..4423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4434..4452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4463..4481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4508..4526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4537..4555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4566..4584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4611..4629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4640..4658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4669..4687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4714..4732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4743..4761
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4772..4790
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4817..4835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4846..4864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4875..4893
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    4960..4985
                     /label=SA
                     /note="splice acceptor site"
     CDS             5351..5374
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5390..5413
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5429..5452
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6451..6471)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6451..6469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6490..6506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6490..6506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6503..6525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6514..6530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6538..6544,6545..6562,6563..6568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6583..6604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6714..6731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6885..7473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6965..6984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7644..8435,8436..8458,1..46))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8267..8286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttccttttca
       61 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      121 ttagaaaaat aaacaaatag ggattccgcg cacatttccc cgaaaagtgc cacctaaatt
      181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
      241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
      481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
      541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
      601 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
      661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      781 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggggtc tcacaggtaa
      841 gcgataagta ttcgatcatg cattggttag cggccgcgaa ttcgcccttg gatccggaat
      901 gaccaccagg cattccgatg ccacgatgga ccatcaggcc atcgagctgc agaagtgacg
      961 accacgcact tcggagtgac agaggaggat cacccctctg gccaccagag tagagcatca
     1021 gcctactcac agagcctacg gaggaccacc ccgtaggagt agagcgagga gcaccagccc
     1081 tcgcgtgtga cgcacgagca tcagccgtgc gatggtaaga tccggaatga ccaccaggca
     1141 ttcctccaag gcgatggacc atcaggccat cgaggactcg aagtgacgac cacgcacttc
     1201 gtgaaggcag aggaggatca cccctctgcc cacaggagta gagcatcagc ctactccaag
     1261 cagctacgga ggaccacccc gtagaagtag ggcgaggagc accagccctc gccatggtgg
     1321 cacgagcatc agccgtgcag ggtatagatc cggaatgacc accaggcatt cctggccaac
     1381 gatggaccat caggccatcg gcatagcgaa gtgacgacca cgcacttcag ggtagcagag
     1441 gaggatcacc cctctggcca cacgagtaga gcatcagcct actccacagg actacggagg
     1501 accaccccgt agaaagtggg cgaggagcac cagccctcgc cgtgatggca cgagcatcag
     1561 ccgtgcgtta ggaagatctc gattcgatgc agcctagtta ggggtcgact agtctactca
     1621 ctatctctat cactgatagg gaaaccttcc tctctctatc actgataggg accttttccc
     1681 ttctctatca ctgataggga cccatattct cctagtttaa cttctatctc tatcactgat
     1741 agggaaactc acttctctct atcactgata gggaccttat cccctctcta tcactgatag
     1801 ggatattata tttcctagta ttcacctcct ctctatcact gatagggatt caccattttc
     1861 tctatcactg atagggatat ttccttctct ctatcactga tagggacata tttttcccta
     1921 gtcaatcctc actctctatc actgataggg actaacaaaa ttctctatca ctgataggga
     1981 aaaccaaaca tctctatcac tgatagggat catcattttc ctagtataac catactctct
     2041 atcactgata gggatcaccc ccactctcta tcactgatag ggattacaca acttctctat
     2101 cactgatagg gactctcttt atcctagtca cattaacatc tctatcactg atagggacac
     2161 cttttcatct ctatcactga tagggacatc cattattctc tatcactgat agggacatta
     2221 actctcctag taaaatcccc atctctatca ctgataggga aacctcatca tctctatcac
     2281 tgatagggaa tctattccat ctctatcact gatagggata tcttttatcc tagtcctaac
     2341 atcatctcta tcactgatag ggaacccaaa ctttctctat cactgatagg gaccaattta
     2401 cctctctatc actgataggg aatcttctta tcctagtaca aaactaatct ctatcactga
     2461 tagggaatcc ctcccttctc tatcactgat agggatacct ttccttctct atcactgata
     2521 gggaattact tttacctagt aatacctcaa tctctatcac tgatagggaa aatcactaat
     2581 ctctatcact gatagggatt tttattattc tctatcactg atagggaact cctttaacct
     2641 agtctattcc atttctctat cactgatagg gattcactac cctctctatc actgataggg
     2701 attatttctt ctctctatca ctgataggga atttttcctt cctagtacca taaccctctc
     2761 tatcactgat agggaacacc aatattctct atcactgata gggacattat ttattctcta
     2821 tcactgatag ggaattatta ctccctagtt cttacattat ctctatcact gatagggaac
     2881 ccctctcctc tctatcactg atagggatat caatcactct ctatcactga tagggaccta
     2941 ttatacccta gtccaatata tatctctatc actgataggg accacacttc ttctctatca
     3001 ctgataggga tcaccttata tctctatcac tgatagggac actctctatc ctagtccaat
     3061 ccttttctct atcactgata gggataaaca catctctcta tcactgatag ggatacattt
     3121 tactctctat cactgatagg gattctccct ttcctagtct taacttcttc tctatcactg
     3181 atagggattt ttccaattct ctatcactga tagggaatca cttcattctc tatcactgat
     3241 agggattatt tctcccctag ttcactccta ctctctatca ctgataggga ctcccaatat
     3301 tctctatcac tgatagggat atttttctct ctctatcact gatagggaca cctcctctcc
     3361 tagttaccac aacttctcta tcactgatag ggaatactct caatctctat cactgatagg
     3421 gatttctatt tctctctatc actgataggg aataccctct acctagtaac aaatctttct
     3481 ctatcactga tagggaataa tatatctctc tatcactgat agggatctta taccctctct
     3541 atcactgata gggaccttcc acctcctagt taatcaaata tctctatcac tgatagggat
     3601 aatctacttt ctctatcact gatagggaaa ctcatccatc tctatcactg atagggattt
     3661 ttaacttcct agtatccatt ctatctctat cactgatagg gataatacaa actctctatc
     3721 actgataggg atactctcct ttctctatca ctgataggga ccttattttc cctagtataa
     3781 tccctatctc tatcactgat agggaacatt aactatctct atcactgata gggattttat
     3841 tccttctcta tcactgatag ggaacttacc tatcctagta tacccataat ctctatcact
     3901 gatagggaaa ccaaaatttc tctatcactg atagggaatc caattactct ctatcactga
     3961 tagggatttc tatctcccta gtttaaaacc aatctctatc actgataggg aacccctaaa
     4021 ctctctatca ctgataggga tttctcttat tctctatcac tgatagggac cttctctcac
     4081 ctagtctact cactatctct atcactgata gggaaacctt cctctctcta tcactgatag
     4141 ggaccttttc ccttctctat cactgatagg gacccatatt ctcctagtac actataaatc
     4201 tctatcactg atagggaaaa cataccttct ctatcactga tagggactat attttttctc
     4261 tatcactgat agggatctaa tacttcctag tactttaaat atctctatca ctgataggga
     4321 atctactaaa tctctatcac tgatagggac ccataaactt ctctatcact gatagggata
     4381 ttctttaacc tagtacaata ccattctcta tcactgatag ggacccccct aattctctat
     4441 cactgatagg gaattttttt cctctctatc actgataggg attatctctt ccctagttcc
     4501 tcttaaatct ctatcactga tagggatctc actacttctc tatcactgat agggaaactc
     4561 ataactctct atcactgata gggaaaatta tcttcctagt aaaacaccta tctctatcac
     4621 tgatagggat attataccat ctctatcact gatagggatt tatcattttc tctatcactg
     4681 atagggattt ttaccatcct agttaaaaat atctctctat cactgatagg gatcactaac
     4741 tctctctatc actgataggg aactattcta ttctctatca ctgataggga cactttttaa
     4801 cctagtacat tacccatctc tatcactgat agggaaccta tctcttctct atcactgata
     4861 gggatatttt ttcatctcta tcactgatag ggaaaacttt acacctagga ttaccctgtt
     4921 atccctactc gacatcgatt agctgatacg ataaagcttc tgacctcttc tcttcctccc
     4981 acagggatgt ctgagctacg atacagagat cctcacagtt gagtacggat ttctgcccat
     5041 tggcaaaata gtcgaggaaa ggattgaatg cacagtttac acagtggaca agaatggctt
     5101 tgtctataca cagccaatag cccagtggca taaccgagga gagcaagagg tattcgagta
     5161 ttgtctcgaa gatgggagta tcatccgagc caccaaagac cacaaattca tgacaacaga
     5221 cggacaaatg ctgcctattg acgaaatatt tgagagaggt ttggatctga agcaggttga
     5281 cgggctgccc gccggagccg gtgccggagc tgcaagcggg aaagagaaaa cacatgtttc
     5341 ccggcctagg gattacaagg atgacgacga taagggcact gcaagcagtg actataagga
     5401 tgatgatgac aaagggtctg gtgggggcga ctataaggat gacgatgaca agcggcctag
     5461 gcttaatagc aacatggacg cagaccttta tggatataag tgggctcgcg acaatgtagg
     5521 acagagcggc gctactattt atcggctcta tggaaaaccc gatgctcccg agctgttcct
     5581 gaagcacggc aagggatccg tagcaaacga cgttacagac gaaatggtac gactcaactg
     5641 gttgacagaa tttatgcccc tgcccactat caagcacttc ataagaactc ctgatgacgc
     5701 ttggctgctg acaaccgcaa tccccggtaa aacagcattc caggtccttg aagagtatcc
     5761 agactctggt gagaatattg tggacgcact ggcagtattc ctcaggcggc tccacagtat
     5821 tcctgtttgc aattgtccct ttaactctga cagggtattc aggctcgccc aggcccaatc
     5881 acgaatgaat aatggcttgg ttgatgcaag cgatttcgat gatgaacgca atggctggcc
     5941 tgttgaacaa gtttggaaag aaatgcacaa gctcctgccc ttttcacccg attctgtagt
     6001 gactcacggg gacttcagcc tcgacaattt gatcttcgat gaaggaaagc ttatcggctg
     6061 catagatgtt gggcgcgttg ggatagccga cagataccag gacctggcca tattgtggaa
     6121 ttgtctcggc gagttttccc ctagcttgca aaaacggctc tttcagaagt atggcataga
     6181 taaccccgac atgaataaac ttcaattcca tctgatgctt gacgagttct tcggaactgc
     6241 tggagctgga gctggggctg taaagggagg aggtggatct ggtggaggag gtagtggagg
     6301 aggtggaagt ggtgggggag taaagatcat cagtcgaaaa tccctgggca cccaaaatgt
     6361 atacgatatt ggggtcgaga aagatcacaa ttttctcctg aagaatggtc tggttgcaag
     6421 caattgcttc tgagacccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     6481 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6541 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6601 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6661 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggccgc ttcctcgctc
     6721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcggctgcc acgctcaccg
     7801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatg
//
