LOCUS       sequence-350448-       10178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human RSK3 (RPS6KA2) gene with point 
            mutations (K91A and K444A) that inactivate kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR,
            Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, 
            Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi
            HY
  TITLE     Dual targeting of brain region-specific kinases potentiates 
            neurological rescue in Spinocerebellar ataxia type 1.
  JOURNAL   EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. 
            Epub 2021 Mar 11.
  PUBMED    33709453
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-04-1"; volume: "40(7):e106106. doi"; pages: " 
            10.15252/embj.2020106106. Epub 2021 Mar 11"
COMMENT     Sequence Label: W118-1_hRSK3(RPS6KA2)_K91A_K444A
FEATURES             Location/Qualifiers
     source          1..10178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5373..5397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5428..6016
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5481..5501)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5899..5910)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6030..6529
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6065..6088)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6462..6481
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6550..7149
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6550..6569)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7046..7066
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7287..7520
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7592..7713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7629..7648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7683..7702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7753..7888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7908..7927)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7909..7927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7937..7954)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7937..7953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7946..7968)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8095..8550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8182..8201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8392..8413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8576..8680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8681..8749,8750..9541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8899..8918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9712..10178,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcacca tggacctgag
     3181 catgaagaag ttcgccgtgc gcaggttctt ctctgtgtac ctgcgcagga agtcgcgctc
     3241 caagagctcc agcctgagcc ggctcgagga agaaggtgtc gtgaaggaga tagacatcag
     3301 ccatcatgtg aaggagggct ttgagaaggc agatccttcc cagtttgagc tgctgaaggt
     3361 tttaggacaa ggatcctatg gaaaggtgtt cctggtgagg aaggtgaagg ggtccgacgc
     3421 tgggcagctc tacgccatgg cggtccttaa gaaagccacc ctaaaagttc gggaccgagt
     3481 gagatcgaag atggagagag acatcttggc agaagtgaat caccccttca ttgtgaagct
     3541 tcattatgcc tttcagacgg aaggaaagct ctacctgatc ctggacttcc tgcggggagg
     3601 ggacctcttc acccggctct ccaaagaggt catgttcacg gaggaggatg tcaagttcta
     3661 cctggctgag ctggccttgg ctttagacca tctccacagc ctggggatca tctacagaga
     3721 tctgaagcct gagaacatcc tcctggatga agaggggcac attaagatca cagatttcgg
     3781 cctgagtaag gaggccattg accacgacaa gagagcgtac tccttctgcg ggacgatcga
     3841 gtacatggcg cccgaggtgg tgaaccggcg aggacacacg cagagtgccg actggtggtc
     3901 cttcggcgtg ctcatgtttg agatgctcac ggggtccctg ccgttccagg ggaaggacag
     3961 gaaggagacc atggctctca tcctcaaagc caagctgggg atgccgcagt tcctcagtgg
     4021 ggaggcacag agtttgctgc gagctctctt caaacggaac ccctgcaacc ggctgggtgc
     4081 tggcattgac ggagtggagg aaattaagcg ccatcccttc tttgtgacca tagactggaa
     4141 cacgctgtac cggaaggaga tcaagccacc gttcaaacca gcagtgggca ggcctgagga
     4201 caccttccac tttgaccccg agttcacagc gcggacgccc acagactctc ctggcgtccc
     4261 cccgagtgca aacgctcatc acctgtttag aggattcagc tttgtggcct caagcctgat
     4321 ccaggagccc tcacagcaag atctgcacaa agtcccagtt cacccaatcg tgcagcagtt
     4381 acacgggaac aacatccact tcaccgatgg ctacgagatc aaggaggaca tcggggtggg
     4441 ctcctactca gtgtgcaagc gatgtgtgca taaagccaca gacaccgagt atgccgtggc
     4501 gatcattgat aagagcaaga gagacccctc ggaagagatt gagatcctcc tgcggtacgg
     4561 ccagcacccg aacatcatca ccctcaagga tgtctatgat gatggcaagt ttgtgtacct
     4621 ggtaatggag ctgatgcgtg gtggggagct cctggaccgc atcctccggc agagatactt
     4681 ctcggagcgc gaagccagtg acgtcctgtg caccatcacc aagaccatgg actacctcca
     4741 ttcccagggg gttgttcatc gagacctgaa gccgagtaac atcctgtaca gggatgagtc
     4801 ggggagccca gaatccatcc gagtctgcga cttcggcttt gccaagcagc tgcgcgcggg
     4861 gaacgggctg ctcatgacac cctgctacac ggccaatttc gtggccccgg aggtcctgaa
     4921 gcgtcaaggc tatgatgcgg cgtgtgacat ctggagtttg gggatcctgt tgtacaccat
     4981 gctggcagga tttacccctt ttgcaaatgg gccagacgat acccctgagg agattctggc
     5041 gcggatcggc agtgggaagt atgccctttc tgggggaaac tgggactcga tatctgacgc
     5101 agctaaagac gtcgtgtcca agatgctcca cgtggaccct catcagcgcc tgacggcgat
     5161 gcaagtgctc aaacacccgt gggtggtcaa cagagagtac ctgtccccaa accagctcag
     5221 ccgacaggac gtgcacctgg tgaagggcgc gatggccgcc acctactttg ctctaaacag
     5281 aacacctcag gccccgcggc tggagcccgt gctgtcgtcc aacctggctc agcgcagagg
     5341 catgaagaga ctcacgtcca cgcggttgta gaacccagct ttcttgtaca aagtggttga
     5401 tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga
     5461 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5521 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5581 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5641 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5701 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5761 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5821 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5881 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5941 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6001 tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     6061 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     6121 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     6181 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     6241 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     6301 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     6361 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     6421 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     6481 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     6541 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6601 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6661 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6721 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6781 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6841 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6901 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6961 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7021 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7081 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7141 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     7201 gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7261 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     7321 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7381 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7441 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7501 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7561 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7681 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7801 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7861 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7921 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7981 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8041 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8101 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8161 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8221 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8281 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8341 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8401 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8461 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8521 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8581 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8641 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8701 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8761 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8821 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8881 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8941 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9001 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9061 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9121 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9181 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9241 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9301 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9361 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9421 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9481 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9541 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9601 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10141 ggacaggtat ccggtaagcg gcagggtcgg aacaggag
//
