LOCUS       sequence-350449-        2202 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral transduction of human RSK3 (RPS6KA2) gene with point 
            mutations (K91A and K444A) that inactivate kinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR,
            Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, 
            Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi
            HY
  TITLE     Dual targeting of brain region-specific kinases potentiates 
            neurological rescue in Spinocerebellar ataxia type 1.
  JOURNAL   EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. 
            Epub 2021 Mar 11.
  PUBMED    33709453
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-04-1"; volume: "40(7):e106106. doi"; pages: " 
            10.15252/embj.2020106106. Epub 2021 Mar 11"
COMMENT     Sequence Label: W118-1_hRSK3(RPS6KA2)_K91A_K444A
FEATURES             Location/Qualifiers
     source          1..2202
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacctga gcatgaagaa gttcgccgtg cgcaggttct tctctgtgta cctgcgcagg
       61 aagtcgcgct ccaagagctc cagcctgagc cggctcgagg aagaaggtgt cgtgaaggag
      121 atagacatca gccatcatgt gaaggagggc tttgagaagg cagatccttc ccagtttgag
      181 ctgctgaagg ttttaggaca aggatcctat ggaaaggtgt tcctggtgag gaaggtgaag
      241 gggtccgacg ctgggcagct ctacgccatg gcggtcctta agaaagccac cctaaaagtt
      301 cgggaccgag tgagatcgaa gatggagaga gacatcttgg cagaagtgaa tcaccccttc
      361 attgtgaagc ttcattatgc ctttcagacg gaaggaaagc tctacctgat cctggacttc
      421 ctgcggggag gggacctctt cacccggctc tccaaagagg tcatgttcac ggaggaggat
      481 gtcaagttct acctggctga gctggccttg gctttagacc atctccacag cctggggatc
      541 atctacagag atctgaagcc tgagaacatc ctcctggatg aagaggggca cattaagatc
      601 acagatttcg gcctgagtaa ggaggccatt gaccacgaca agagagcgta ctccttctgc
      661 gggacgatcg agtacatggc gcccgaggtg gtgaaccggc gaggacacac gcagagtgcc
      721 gactggtggt ccttcggcgt gctcatgttt gagatgctca cggggtccct gccgttccag
      781 gggaaggaca ggaaggagac catggctctc atcctcaaag ccaagctggg gatgccgcag
      841 ttcctcagtg gggaggcaca gagtttgctg cgagctctct tcaaacggaa cccctgcaac
      901 cggctgggtg ctggcattga cggagtggag gaaattaagc gccatccctt ctttgtgacc
      961 atagactgga acacgctgta ccggaaggag atcaagccac cgttcaaacc agcagtgggc
     1021 aggcctgagg acaccttcca ctttgacccc gagttcacag cgcggacgcc cacagactct
     1081 cctggcgtcc ccccgagtgc aaacgctcat cacctgttta gaggattcag ctttgtggcc
     1141 tcaagcctga tccaggagcc ctcacagcaa gatctgcaca aagtcccagt tcacccaatc
     1201 gtgcagcagt tacacgggaa caacatccac ttcaccgatg gctacgagat caaggaggac
     1261 atcggggtgg gctcctactc agtgtgcaag cgatgtgtgc ataaagccac agacaccgag
     1321 tatgccgtgg cgatcattga taagagcaag agagacccct cggaagagat tgagatcctc
     1381 ctgcggtacg gccagcaccc gaacatcatc accctcaagg atgtctatga tgatggcaag
     1441 tttgtgtacc tggtaatgga gctgatgcgt ggtggggagc tcctggaccg catcctccgg
     1501 cagagatact tctcggagcg cgaagccagt gacgtcctgt gcaccatcac caagaccatg
     1561 gactacctcc attcccaggg ggttgttcat cgagacctga agccgagtaa catcctgtac
     1621 agggatgagt cggggagccc agaatccatc cgagtctgcg acttcggctt tgccaagcag
     1681 ctgcgcgcgg ggaacgggct gctcatgaca ccctgctaca cggccaattt cgtggccccg
     1741 gaggtcctga agcgtcaagg ctatgatgcg gcgtgtgaca tctggagttt ggggatcctg
     1801 ttgtacacca tgctggcagg atttacccct tttgcaaatg ggccagacga tacccctgag
     1861 gagattctgg cgcggatcgg cagtgggaag tatgcccttt ctgggggaaa ctgggactcg
     1921 atatctgacg cagctaaaga cgtcgtgtcc aagatgctcc acgtggaccc tcatcagcgc
     1981 ctgacggcga tgcaagtgct caaacacccg tgggtggtca acagagagta cctgtcccca
     2041 aaccagctca gccgacagga cgtgcacctg gtgaagggcg cgatggccgc cacctacttt
     2101 gctctaaaca gaacacctca ggccccgcgg ctggagcccg tgctgtcgtc caacctggct
     2161 cagcgcagag gcatgaagag actcacgtcc acgcggttgt ag
//
