LOCUS       sequence-35048-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: 426Gal-FUS-368-526aa-YFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(577..601)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(609..625)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(646..669)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc attaagcttg atatcgaatt cctgcagccc atcaccactt
       61 tgtacaagaa agctgggtca tacggcctct ccctgcgatc ctgtctgtgc tcacccctgg
      121 aatccatctt gccagggcca aagccacctc tgtccccacc accccggccc cctcggaagc
      181 ctccacggtc cccgccgcgg ccccggtagc cgcctcgatc atagcctcct ctgccaccac
      241 gacgatcatc cccgtagtta ccccccatgt gagagccacc tggtccccct cctgggccat
      301 ctggtttagg ggccttacac tggttgcatt cattcctcca agagaagttc atattctcac
      361 aggtgggatt aggacacttc cagtcaccag ctcgctgctg tcctccaccg ccaccacctc
      421 cactgggaaa tcctcctcgg ccaccaccac cactgccacc acctccatag cctccacggc
      481 ccatgggtcc tcctcgccct cggcctccac gaccattgcc accaccccga ttaaagtctg
      541 cccggcgagt agcaaacatt ttgttatctc cttcgaagcc tgcttttttg tacaaacttg
      601 tgatggggga tccactagtt ctagaatccg gggttttttc tccttgacgt taaagtatag
      661 aggtatatta acaatttttt gttgatactt ttattacatt tgaataagaa gtaatacaaa
      721 ccgaaaatgt tgaaagtatt agttaaagtg gttatgcagt ttttgcattt atatatctgt
      781 taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg ctaaaaaact
      841 aatcgcatta tcatcctatg gtt
//
