LOCUS       sequence-350487-        1788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral transduction of human SRPK3 (with an N-terminal Flag tag)
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_Flag-hSRPK3
FEATURES             Location/Qualifiers
     source          1..1788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggactaca aagacgatga cgacaaggtc cagtgtggtg gaattctgca gatatcaaca
       61 agtttgtaca aaaaagttgg caccatgagc gccagcacgg gcggtggtgg ggacagcggc
      121 ggcagcggcg gcagtagcag cagctcacag gcctcctgcg ggcccgagtc ctcgggctcc
      181 gaactagccc tggccacacc ggtgcctcag atgctgcagg gccttctggg ctccgacgac
      241 gaggaacagg aagaccccaa agactactgc aagggcggct accaccctgt gaagatcggc
      301 gacgtgttca atgggcggta ccacgtggtg cgcaaactgg gctggggcca cttctccacc
      361 gtctggctct gctgggacat ccagcgcaag cgctttgtgg ccctcaaagt ggtgaagagt
      421 gcggggcatt acacggagac agctgtggat gagatcaagc tcctgaaatg tgtccgggac
      481 agcgacccca gtgaccccaa aagagagacc attgtccagc tcattgatga cttcaggatc
      541 tcaggagtca atggagtcca tgtgtgcatg gtgctggagg tgctgggcca ccagctcctc
      601 aaatggatca tcaagtccaa ctaccagggc ctgcccgtgc cctgcgtgaa gagcatcgtg
      661 aggcaggtgc tgcacggcct ggactacctc cacaccaagt gcaagatcat ccacacggac
      721 atcaagcccg agaacatctt gctgtgtgtg ggggacgctt acatcaggcg cctggctgcc
      781 gaggccacgg agtggcaaca ggcaggggcg ccgcccccct cccgctccat agtcagcact
      841 gccccccagg aggtcttgca gaccggtaag ctgtccaaaa acaagaggaa gaagatgagg
      901 cgcaaacgga aacagcagaa gcggctgctg gaggagcggc tgcgggacct gcagaggctg
      961 gaggccatgg aggctgccac ccaggctgag gactctggct tgagactaga cgggggcagc
     1021 ggctccacat cctcttcagg ctgtcacccc gggggcgcca gagcaggtcc ctccccagcc
     1081 tcttcctccc ccgccccagg gggcggccgt agcctcagcg cgggctcaca gacctcaggc
     1141 ttctccggct ccctcttctc tcctgcctcc tgctccatcc tctccggctc gtccaatcag
     1201 cgagagaccg ggggcctcct gtcgcctagc acaccattcg gtgcctcgaa cctcctggtg
     1261 aaccccctgg agccccaaaa tgcagataag atcaagatca agatcgcaga cctgggcaac
     1321 gcctgctggg tgcacaagca cttcacggaa gacatccaga ctcggcagta ccgggccgtc
     1381 gaggtgctga tcggcgccga atacggcccc ccggcagaca tctggagcac agcctgcatg
     1441 gccttcgagc tggccactgg tgactacctg ttcgagccgc attctggaga agactacagt
     1501 cgtgatgagg accacatcgc tcacatagtg gagcttctgg gggacatccc cccagccttc
     1561 gccctctcag gccgctattc ccgggagttc ttcaaccgga gaggagagct gcggcacatc
     1621 cacaatctca agcactgggg cctgtacgag gtactcatgg aaaagtacga gtggccccta
     1681 gagcaggcca cacagttcag cgcctttctg ctgcccatga tggagtacat ccccgaaaag
     1741 cgggccagtg ccgctgactg cctccagcac ccctggctca acccctag
//
