LOCUS       sequence-350497-        2085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM4 (with a C-terminal Flag tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM4-Flag
FEATURES             Location/Qualifiers
     source          1..2085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2059..2082
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgatggatg catcaaaaga gctgcaagtt ctccacattg acttcttgaa tcaggacaac
       61 gccgtttctc accacacatg ggagttccaa acgagcagtc ctgtgttccg gcgaggacag
      121 gtgtttcacc tgcggctggt gctgaaccag cccctacaat cctaccacca actgaaactg
      181 gaattcagca cagggccgaa tcctagcatc gccaaacaca ccctggtggt gctcgacccg
      241 aggacgccct cagaccacta caactggcag gcaacccttc aaaatgagtc tggcaaagag
      301 gtcacagtgg ctgtcaccag ttcccccaat gccatcctgg gcaagtacca actaaacgtg
      361 aaaactggaa accacatcct taagtctgaa gaaaacatcc tataccttct cttcaaccca
      421 tggtgtaaag aggacatggt tttcatgcct gatgaggacg agcgcaaaga gtacatcctc
      481 aatgacacgg gctgccatta cgtgggggct gccagaagta tcaaatgcaa accctggaac
      541 tttggtcagt ttgagaaaaa tgtcctggac tgctgcattt ccctgctgac tgagagctcc
      601 ctcaagccca cagataggag ggaccccgtg ctggtgtgca gggccatgtg tgctatgatg
      661 agctttgaga aaggccaggg cgtgctcatt gggaattgga ctggggacta cgaaggtggc
      721 acagccccat acaagtggac aggcagtgcc ccgatcctgc agcagtacta caacacgaag
      781 caggctgtgt gctttggcca gtgctgggtg tttgctggga tcctgactac agtgctgaga
      841 gcgttgggca tcccagcacg cagtgtgaca ggcttcgatt cagctcacga cacagaaagg
      901 aacctcacgg tggacaccta tgtgaatgag aatggcgaga aaatcaccag tatgacccac
      961 gactctgtct ggaatttcca tgtgtggacg gatgcctgga tgaagcgacc ggatctgccc
     1021 aagggctacg acggctggca ggctgtggac gcaacgtcgc aggagcgaag ccagggtgtc
     1081 ttctgttgtg ggccatcacc actgaccgcc atccgcaaag gtgacatctt tattgtctat
     1141 gacaccagat tcgtcttctc agaagtgaat ggtgacaggc tcatctggtt ggtgaagatg
     1201 gtgaatgggc aggaggagtt acacgtaatt tcaatggaga ccacaagcat cgggaaaaac
     1261 atcagcacca aggcagtggg ccaagacagg cggagagata tcacctatga gtacaagtat
     1321 ccagaaggct cctctgagga gaggcaggtc atggatcatg ccttcctcct tctcagttct
     1381 gagagggagc acagacgacc tgtaaaagag aactttcttc acatgtcggt acaatcagat
     1441 gatgtgctgc tgggaaactc tgttaatttc accgtgattc ttaaaaggaa gaccgctgcc
     1501 ctacagaatg tcaacatctt gggttccttt gaactacagt tgtacactgg caagaagatg
     1561 gcaaaactgt gtgacctcaa taagacctcg cagatccaag gtcaagtatc agaagtgact
     1621 ctgaccttgg actccaagac ctacatcaac agcctggcta tattagatga tgagccagtt
     1681 atcagaggtt tcatcattgc ggaaattgtg gagtctaagg aaatcatggc ctctgaagta
     1741 ttcacgtctt tccagtaccc tgagttctct atagagttgc ctaacacagg cagaattggc
     1801 cagctacttg tctgcaattg tatcttcaag aataccctgg ccatcccttt gactgacgtc
     1861 aagttctctt tggaaagcct gggcatctcc tcactacaga cctctgacca tgggacggtg
     1921 cagcctggtg agaccatcca atcccaaata aaatgcaccc caataaaaac tggacccaag
     1981 aaatttatcg tcaagttaag ttccaaacaa gtgaaagaga ttaatgctca gaagattgtt
     2041 ctcatcacca agggcggcga ctacaaagac gatgacgaca agtga
//
