LOCUS       sequence-350500-       10130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM6 (with a C-terminal Flag tag) 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM6-Flag
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5297..5320
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5325..5349)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5380..5968
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5433..5453)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5851..5862)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5982..6481
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6017..6040)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6414..6433
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6502..7101
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6502..6521)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6998..7018
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7239..7472
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7544..7665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7581..7600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7635..7654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7705..7840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7860..7879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7861..7879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7889..7906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7889..7905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7898..7920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8047..8502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8134..8153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8344..8365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8528..8632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8633..8701,8702..9493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8851..8870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9664..10130,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatggcagg
     3181 gatcagagtc accaaggtgg actggcagcg gtcgaggaat ggcgctgccc accacaccca
     3241 ggagtacccc tgccctgagc tggtggttcg caggggccag tcgttcagcc tcacgctgga
     3301 gctgagcaga gccctggact gtgaggagat cctcatcttc acgatggaga caggaccccg
     3361 ggcttctgag gccctccaca ccaaagctgt gttccagaca tcggagctgg agcggggtga
     3421 gggctggaca gcagcaaggg aggctcagat ggagaaaact ctgaccgtca gtctcgccag
     3481 ccctcccagt gctgtcattg gccgctacct gctgagcatc aggctttcct ctcaccgcaa
     3541 acacagcaac cggaggctgg gcgagtttgt tctccttttc aacccatggt gtgcagagga
     3601 cgatgtgttt ctggcctcag aggaggagag acaggagtac gtgctcagcg acagcggcat
     3661 catcttccga ggcgtggaga agcacatacg agcccagggc tggaactacg ggcagtttga
     3721 ggaggacatc ctgaacatct gcctctccat cctggatcga agccccggtc accaaaacaa
     3781 cccagccacc gacgtgtcct gccgccacaa ccccatctac gtcaccaggg tcatcagtgc
     3841 catggtgaac agcaacaacg accgaggtgt ggtgcaagga cagtggcagg gcaagtacgg
     3901 cggcggcacc agcccgctgc actggcgcgg cagcgtggcc attctgcaga agtggctcaa
     3961 gggcaggtac aagccagtca agtacggcca gtgctgggtc ttcgccggag tcctgtgcac
     4021 agtcctcagg tgcttgggga tagccacacg ggtcgtgtcc aacttcaact cagcccacga
     4081 cacagaccag aacctgagtg tggacaaata cgtggactcc ttcgggcgga ccctggagga
     4141 cctgacagaa gacagcatgt ggaatttcca tgtctggaat gagagctggt ttgcccggca
     4201 ggacctaggc ccctcttaca atggctggca ggttctggat gccacccccc aggaggagag
     4261 tgaaggtgtg ttccggtgcg gcccagcctc agtcaccgcc atccgcgagg gtgatgtgca
     4321 cctggctcac gatggcccct tcgtgtttgc ggaggtcaac gccgactaca tcacctggct
     4381 gtggcacgag gatgagagcc gggagcgtgt atactcaaac acgaagaaga ttgggagatg
     4441 catcagcacc aaggcggtgg gcagtgactc ccgcgtggac atcactgacc tctacaagta
     4501 tccggaaggg tcccggaaag agaggcaggt gtacagcaag gcggtgaaca ggctgttcgg
     4561 cgtggaagcc tctggaagga gaatctggat ccgcagggct gggggtcgct gtctctggcg
     4621 tgacgacctc ctggagcctg ccaccaagcc cagcatcgct ggcaagttca aggtgctaga
     4681 gcctcccatg ctgggccacg acctgagact ggccctgtgc ttggccaacc tcacctcccg
     4741 ggcccagcgg gtgagggtca acctgagcgg tgccaccatc ctctataccc gcaagccagt
     4801 ggcagagatc ctgcatgaat cccacgccgt gaggctgggg ccgcaagaag agaagagaat
     4861 cccaattaca atatcttact ctaagtataa agaagacctg acagaggaca agaagatcct
     4921 gttggctgcc atgtgccttg tcaccaaagg agagaagctt ctggtggaga aggacattac
     4981 tctagaggac ttcatcacca tcaaggttct gggcccagcc atggtgggag tggcagttac
     5041 agtggaagtg acagtagtca accccctcat agagagagtg aaggactgtg cgctgatggt
     5101 ggagggcagc ggccttctcc aggaacagct cagcatcgac gtgcctaccc tggagcctca
     5161 ggagagggcc tcagtccagt ttgacatcac cccctccaaa agtggcccaa ggcagctgca
     5221 ggtggacctt gtaagccctc acttcccgga catcaagggc tttgtgatcg tccatgtggc
     5281 cactgccaag ggcggcgact acaaagacga tgacgacaag tgagacccag ctttcttgta
     5341 caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
     5401 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5461 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5521 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5581 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5641 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5701 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5761 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5821 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5881 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5941 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt
     6001 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6061 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6121 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6181 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6241 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6301 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     6361 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     6421 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     6481 gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     6541 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6601 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6661 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6721 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6781 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6841 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6901 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6961 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7021 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7081 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     7141 gcacgacccc atgcatctcg agggcccggt acctttaaga ccaatgactt acaaggcagc
     7201 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca
     7261 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7321 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7381 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7441 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7501 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7681 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7741 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7801 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7861 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8041 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8221 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     8341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     8401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8461 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     8521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
//
