LOCUS       sequence-350503-        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral transduction of human TGM2 transcript variant 2 with a 
            point mutation (C277S) that inactivate the enzyme and also with a 
            C-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA,
            Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, 
            Botas J, Zoghbi HY
  TITLE     Cross-species genetic screens identify transglutaminase 5 as a 
            regulator of polyglutamine-expanded ataxin-1.
  JOURNAL   J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 
            10.1172/JCI156616.
  PUBMED    35499073
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2022-05-2"; volume: "132(9). pii: 156616. doi"; 
            pages: " 10.1172/JCI156616"
COMMENT     Sequence Label: W118-1_hTGM2_C277S-Flag
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1651..1674
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggccgagg agctggtctt agagaggtgt gatctggagc tggagaccaa tggccgagac
       61 caccacacgg ccgacctgtg ccgggagaag ctggtggtgc gacggggcca gcccttctgg
      121 ctgaccctgc actttgaggg ccgcaactac gaggccagtg tagacagtct caccttcagt
      181 gtcgtgaccg gcccagcccc tagccaggag gccgggacca aggcccgttt tccactaaga
      241 gatgctgtgg aggagggtga ctggacagcc accgtggtgg accagcaaga ctgcaccctc
      301 tcgctgcagc tcaccacccc ggccaacgcc cccatcggcc tgtatcgcct cagcctggag
      361 gcctccactg gctaccaggg atccagcttt gtgctgggcc acttcatttt gctcttcaac
      421 gcctggtgcc cagcggatgc tgtgtacctg gactcggaag aggagcggca ggagtatgtc
      481 ctcacccagc agggctttat ctaccagggc tcggccaagt tcatcaagaa cataccttgg
      541 aattttgggc agtttgaaga tgggatccta gacatctgcc tgatccttct agatgtcaac
      601 cccaagttcc tgaagaacgc cggccgtgac tgctcccgcc gcagcagccc cgtctacgtg
      661 ggccgggtgg tgagtggcat ggtcaactgc aacgatgacc agggtgtgct gctgggacgc
      721 tgggacaaca actacgggga cggcgtcagc cccatgtcct ggatcggcag cgtggacatc
      781 ctgcggcgct ggaagaacca cggctgccag cgcgtcaagt atggccagtc ctgggtcttc
      841 gccgccgtgg cctgcacagt gctgaggtgc ctgggcatcc ctacccgcgt cgtgaccaac
      901 tacaactcgg cccatgacca gaacagcaac cttctcatcg agtacttccg caatgagttt
      961 ggggagatcc agggtgacaa gagcgagatg atctggaact tccactgctg ggtggagtcg
     1021 tggatgacca ggccggacct gcagccgggg tacgagggct ggcaggccct ggacccaacg
     1081 ccccaggaga agagcgaagg gacgtactgc tgtggcccag ttccagttcg tgccatcaag
     1141 gagggcgacc tgagcaccaa gtacgatgcg ccctttgtct ttgcggaggt caatgccgac
     1201 gtggtagact ggatccagca ggacgatggg tctgtgcaca aatccatcaa ccgttccctg
     1261 atcgttgggc tgaagatcag cactaagagc gtgggccgag acgagcggga ggatatcacc
     1321 cacacctaca aatacccaga ggggtcctca gaggagaggg aggccttcac aagggcgaac
     1381 cacctgaaca aactggccga gaaggaggag acagggatgg ccatgcggat ccgtgtgggc
     1441 cagagcatga acatgggcag tgactttgac gtctttgccc acatcaccaa caacaccgct
     1501 gaggagtacg tctgccgcct cctgctctgt gcccgcaccg tcagctacaa tgggatcttg
     1561 gggcccgagt gtggcaccaa gtacctgctc aacctcaacc tggagccttt ctctggtaaa
     1621 gccctgtgtt cctggagcat ttgtggcggc gactacaaag acgatgacga caagtga
//
