LOCUS       sequence-350509-        9907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3192
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2661..2685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2693..2734
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2845..3433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2898..2918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3316..3327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3505..3738
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3816..3937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3853..3872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3907..3926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3977..4112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4132..4151)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4133..4151)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4161..4178)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4161..4177)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4170..4192)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4319..4774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4406..4425)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4616..4637
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4800..4904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4905..4973,4974..5765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5123..5142)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5936..6524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6425..6444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6678..6695
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6812..6833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6848..6853,6854..6871,6872..6878)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6886..6902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6891..6913
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6910..6926
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6910..6926
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6945..6965
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6947..6965
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6993..7219
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7220..7400
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7447..7572
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8065..8298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8483..8527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8758..8968
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8780..8976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8827..8962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9008..9610
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9008..9027)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9504..9524
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9653..9770
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggccag cactattaag gaagccttat cagttgtgag
     1261 tgaggaccag tcgttgtttg agtgtgccta cggaacgcca cacctggcta agacagagat
     1321 gaccgcgtcc tcctccagcg actatggaca gacttccaag atgagcccac gcgtccctca
     1381 gcaggattgg ctgtctcaac ccccagccag ggtcaccatc aaaatggaat gtaaccctag
     1441 ccaggtgaat ggctcaagga actctcctga tgaatgcagt gtggccaaag gcgggaagat
     1501 ggtgggcagc ccagacaccg ttgggatgaa ctacggcagc tacatggagg agaagcacat
     1561 gccaccccca aacatgacca cgaacgagcg cagagttatc gtgccagcag atcctacgct
     1621 atggagtaca gaccatgtgc ggcagtggct ggagtgggcg gtgaaagaat atggccttcc
     1681 agacgtcaac atcttgttat tccagaacat cgatgggaag gaactgtgca agatgaccaa
     1741 ggacgacttc cagaggctca cccccagcta caatgccgac atccttctct cacatctcca
     1801 ctacctcaga gagactcctc ttccacattt gacttcagat gatgttgata aagccttaca
     1861 aaactctcca cggttaatgc atgctagaaa cacagggggt gcagctttta ttttcccaaa
     1921 tacttcagta tatcctgaag ctacgcaaag aattacaact aggccagatt taccatatga
     1981 gccccccagg agatcagcct ggaccggtca cggccacccc acgccccagt cgaaagctgc
     2041 tcaaccatct ccttccacag tgcccaaaac tgaagaccag cgtcctcagt tagatcctta
     2101 tcagattctt ggaccaacaa gtagccgcct tgcaaatcca ggcagtggcc agatccagct
     2161 ttggcagttc ctcctggagc tcctgtcgga cagctccaac tccagctgca tcacctggga
     2221 aggcaccaac ggggagttca agatgacgga tcccgacgag gtggcccggc gctggggaga
     2281 gcggaagagc aaacccaaca tgaactacga taagctcagc cgcgccctcc gttactacta
     2341 tgacaagaac atcatgacca aggtccatgg gaagcgctac gcctacaagt tcgacttcca
     2401 cgggatcgcc caggccctcc agccccaccc cccggagtca tctctgtaca agtacccctc
     2461 agacctcccg tacatgggct cctatcacgc ccacccacag aagatgaact ttgtggcgcc
     2521 ccaccctcca gccctccccg tgacatcttc cagttttttt gctgccccaa acccatactg
     2581 gaattcacca actgggggta tataccccaa cactaggctc cccaccagcc atatgccttc
     2641 tcatctgggc acttactact acccaacttt cttgtacaaa gtggttgata tcggtaagcc
     2701 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga
     2761 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagctaa ataaattact
     2821 aaaggacgca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2881 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2941 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3001 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3061 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3121 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3181 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3241 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3301 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3361 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3421 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3481 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3541 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3721 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3781 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3841 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3901 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     3961 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4081 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4141 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4201 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4261 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4321 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4381 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4441 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4501 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4561 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4621 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4681 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4741 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4801 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4861 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4921 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4981 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5041 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5101 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5161 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5221 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5281 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5341 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5401 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5461 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5521 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5581 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5641 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5701 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5761 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5821 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5881 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5941 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6001 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6061 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6121 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6181 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6241 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6301 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6361 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6421 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6481 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6541 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6601 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6661 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6721 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6781 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6841 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6901 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     6961 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7021 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7081 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7141 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7201 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7261 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7321 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7381 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7441 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7501 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7561 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     7621 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     7681 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7741 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7801 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7861 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     7921 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     7981 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8041 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8101 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8161 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8221 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8281 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     8341 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     8401 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     8461 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     8521 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     8581 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     8641 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     8701 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8761 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     8821 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8881 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8941 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     9001 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     9061 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9121 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9181 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     9241 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9301 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9361 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9421 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9481 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9541 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9601 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9661 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9721 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9781 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
     9841 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     9901 gggaatt
//
