LOCUS       sequence-350515-        9781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding VPS26C and EmGFP-Bsd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: VPS26C_pcDNA6.2/EmGFP-Bsd
FEATURES             Location/Qualifiers
     source          1..9781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4737..4761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4787..4828
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4868..4887)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4912..4960
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5162..5590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5249..5268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5459..5480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5605..6104
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5640..5663)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6037..6056
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6118..6165
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(6184..6186,6187..6189,6190..6900)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6229..6250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6490..6509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6837..6858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6901..7296
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7454..7575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7491..7510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7545..7564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7624..7640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7624..7640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7637..7659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7648..7664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7672..7678,7679..7696,7697..7702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7717..7738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7855..7872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8026..8614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8106..8125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8785..9576,9577..9645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9408..9427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9646..9750)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctatca acaagtttgt acaaaaaagc aggctggtac cggtccggaa ttcccgggat
      961 atcgtcgacc cacgcgtccg cccacgcgtc cgcccacgcg tccgcgcacc cctgagggtg
     1021 accggaaccg ctcccgcggg aagggcaggg tgcggcgcct ggccgcagac agacaagggg
     1081 gcgccggctc ttcagaaccc cagggagaaa gcggtggagg gctagagttg aggagactaa
     1141 ggacatttga ggagggcagg cattaccaag ttaaaacctc ctgaaagaca actccattgg
     1201 gcgaccgtct gcgccgcaca gcctgacggg agcgtcatcc ttcctgcgca ggctgcaacc
     1261 gacagacagg ctcggctccg cctccagccg cggggcacgc ggggagagcc gttcctccct
     1321 ccctccgcta acccggacag caaaggagca tttccggcgt aagactgtcc ctacggaagg
     1381 ggtggggcta gcttggcccc gtctttcacg cccagagggg gcggggtgaa ccgtgacagc
     1441 gcttcggccc gcactaaggc cggttcttgt gccggaagga ggaaggcgtg gggcattcgc
     1501 ccctcggagc tagggagtgt gtgcgacgcc gctgcgaggt cacgtgagcc actgccggca
     1561 gagagggaaa ggggcggggc ccagaacgaa gcggggaggc gccccttgtt tccctggggt
     1621 cacgcgcagc cggaagtggc ggctgctgcg gagaattgga gatggggacc gccctggaca
     1681 tcaagattaa aagagcgaat aaagtttatc acgccgggga agtgctctct ggcgtggtgg
     1741 tcatatcgag taaggattca gtccaacacc agggagtgtc tttgaccatg gaaggaactg
     1801 taaacctcca gctcagtgcc aaaagtgtgg gtgtgtttga agctttttat aattctgtta
     1861 agcctatcca gattatcaac agcaccatag aaatggtgaa gccggggaaa tttcccagcg
     1921 gcaaaacaga aatccctttt gaatttcctc tgcacttgaa gggtaacaaa gttctgtatg
     1981 agacgtatca tggcgtgttt gtcaacattc agtatacact gcgctgtgac atgaagcggt
     2041 ctctgttggc caaggacttg acaaagacct gtgaatttat cgttcactcc gctcctcaga
     2101 aggggaagtt tactcccagt cccgtggact tcacgattac acctgaaacc ttacagaacg
     2161 tcaaagagag agctttgctt cccaaatttc tccttcgagg acatctcaac tcaacaaact
     2221 gtgtcatcac gcagccacta acgggagagc tggtggtgga gagctcggaa gccgccatca
     2281 gaagcgtgga gctgcagctg gtgcgcgtgg agacgtgcgg gtgtgcagaa ggctatgccc
     2341 gcgacgccac ggagattcag aacattcaga tcgccgacgg ggatgtgtgc aggggcctct
     2401 ctgtccccat ctacatggtc ttccctaggc tgttcacctg ccctacactg gagaccacca
     2461 acttcaaagt ggaatttgag gttaacatcg tggtgctgct tcaccctgac cacctcatca
     2521 cggagaactt cccgctgaag ctctgcagga tatagcccgg aggagggaag catagagaac
     2581 gggagtggcc atctggaaat ccagctggtt atccaaatcc taaggggagc tacagccagc
     2641 ggcatatact tgtttttgtg attattctgt atcagaaatg aaacagaccc tcaaattaac
     2701 tttccttcct catttcttga ggcttctgct tccaacaggc acctctaatc agaccttttc
     2761 tttgaaattc aacaagattt cttaatgcta tttgccaaga ccatttcaca gaaaacattg
     2821 actgtggctc ttgccttatc tgttcctttt taggtacagt aaaacaattg tgacagcagt
     2881 ttgagcttgc tggagagtgg catcatgggg acaaaaggaa acctctgact tgctaatgga
     2941 tgtagccagg gactccccat agcaaagggt ctgtggccag ttgacatcca ggatggctgc
     3001 aagcgcactt gatggtcagg aagtttgcag atactcgcca aggcagagcg caaagtgcta
     3061 gccactggaa atgcatgact tccctccacc cctactctat tctgtagttt tttggttttg
     3121 tttctgagac ggagtctcag tctgtcaccc aggctggagt gatctcagct cactgcaacc
     3181 tccacctccc aggttcaagc gactctcctg cctcagcctc ccgagtagtt gggattacag
     3241 gtgactgcca ccgtgcccgg ctaatgtttg tatttttagt agagacgggg cttcaccatc
     3301 ttggccaggc tggtcttgaa ctcctgacct cgtgacccac ccgccttggc ctcccaaagt
     3361 gctgggatta caggtgtgag ccaccacacc cagcctctgt agttcttttt acaacatttt
     3421 tcattataac tttaaatttt ttaagcaact ggaaaagtgt tccttgctct cttgggggga
     3481 tttggctggt gccgaagtgt ttctgaagtc tcaagaactg ccataaaatc tcacgctgcc
     3541 atttccctga acagatacat acatagagag agacagtttt ccaaactgtg tcacgcaggc
     3601 tgagtgcact ggcaggatca cagctcacgg cagcctcaac ctccctggct caagcgatcc
     3661 ctcccctcag cctcctgagt agctgagact acaggtgagt gccaccacac tcagctaatt
     3721 tttaaatttt ttgtagacag ggtctcccta tgttgcccag gctggtcttg aactcctaga
     3781 ctcaagtgat cctcctgtct tggcctccca aagtgctgag attacaggtg tgagccactg
     3841 tgcccagcag tttcccagaa tatatttaaa tgcaaagtta catgagggga aaacatgtat
     3901 gtttgctcct gttgttactg ggtaggttct gaacagcaga aacccatgtg cagggtgggc
     3961 tggtgaaggc ccctctccgc aaggtggtag caggaaaagg tccttgactt gatgaatttg
     4021 gtctgcctct gagccactgg aggaagctgt tttgagccag ggttttttgg cctaaagcca
     4081 gcatttcctc agtctccctt tgtggttcga aggatatgga ctattgcaat acatttcttc
     4141 cttcaaatcc tgccactgtt ttgttggccc acaactaata ggacctcaaa ataagccatg
     4201 ctgctttgca cacacactag ccttcttttg tacttttctt ctggatgggc ttggccaaaa
     4261 caggctcagg ccaaagacct cccaagctgt atgtacttcc agtatcctga aacagtgttt
     4321 ggtgacataa tgccaagggt aaacaagcct gatttaggca ctgctttatc caggggcttc
     4381 acccatgaaa ttaataaaac ttatctgagt cacttgaaac ttggttccca gaaaacacat
     4441 ttctggttta taatctcctt ttatgctcac ctgacattaa ttatctatcc ttgatgatgt
     4501 gtttaaactg agtagcagaa aacagaggcc acactttctg ggaaatttta aaggaagaaa
     4561 ccatttttaa tgagatgaaa atatttaacg aatttaaaaa gctaatgaca attttgagaa
     4621 aaggtttggg atgtatattg ctatgtaatt taataaactg attttatgga tataaaaaaa
     4681 aaaaaaaaaa aggcggccgc tctagagtat ccctcgaggg gcccaagctt acgcgtaccc
     4741 agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc
     4801 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt aaacggggga
     4861 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     4921 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4981 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5041 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5101 gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc
     5161 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5221 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5281 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5341 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5401 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5461 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5521 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5581 aacgcgaatt atcctcgagc taccgggtag gggaggcgct tttcccaagg cagtctggag
     5641 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     5701 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     5761 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     5821 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     5881 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     5941 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     6001 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6061 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgactcta gacacgtgtt
     6121 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     6181 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     6241 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6301 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6361 accctcgtga ccaccttcac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     6421 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6481 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6541 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6601 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag
     6661 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc
     6721 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6781 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6841 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6901 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     6961 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     7021 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     7081 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     7141 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     7201 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     7261 gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga
     7321 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7381 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7441 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7501 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7561 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7621 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7681 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7741 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7801 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7861 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7921 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7981 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8041 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8101 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8161 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8221 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8281 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8341 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8401 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8461 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8521 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8581 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8641 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8701 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8761 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8821 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8881 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8941 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9001 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9061 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9121 ctcgtcgttt ggtatggctt cattcagctt cggttcccaa cgatcaaggc gagttacatg
     9181 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9241 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9301 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9361 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9421 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9481 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9541 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9601 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9661 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9721 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9781 c
//
