LOCUS       sequence-350517-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3195
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1595..1624
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1598..1627
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(2622..2646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2654..2695
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2806..3394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2859..2879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3277..3288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3466..3699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3777..3898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3814..3833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3868..3887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3938..4073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4093..4112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4094..4112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4122..4139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4122..4138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4131..4153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4280..4735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4367..4386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4577..4598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4761..4865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4866..4934,4935..5726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5084..5103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5897..6485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6386..6405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6639..6656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6773..6794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6809..6814,6815..6832,6833..6839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6847..6863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6852..6874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6871..6887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6871..6887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6906..6926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6908..6926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6954..7180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7181..7361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7408..7533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8026..8259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8444..8488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8719..8929
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8741..8937
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8788..8923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8969..9571
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8969..8988)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9465..9485
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9614..9731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgaa cgcgcagctg accatggaag cgatcggcga
     1261 gctgcacggg gtgagccatg agccggtgcc cgcccctgcc gacctgctgg gcggcagccc
     1321 ccacgcgcgc agctccgtgg cgcaccgcgg cagccacctg ccccccgcgc acccgcgctc
     1381 catgggcatg gcgtccctgc tggacggcgg cagcggcggc ggagattacc accaccacca
     1441 ccgggcccct gagcacagcc tggccggccc cctgcatccc accatgacca tggcctgcga
     1501 gactccccca ggtatgagca tgcccaccac ctacaccacc ttgacccctc tgcagccgct
     1561 gcctcccatc tccacagtct cggacaagtt cccccaccat caccaccacc accatcacca
     1621 ccaccacccg caccaccacc agcgcctggc gggcaacgtg agcggtagct tcacgctcat
     1681 gcgggatgag cgcgggctgg cctccatgaa taacctctat accccctacc acaaggacgt
     1741 ggccggcatg ggccagagcc tctcgcccct ctccagctcc ggtctgggca gcatccacaa
     1801 ctcccagcaa gggctccccc actatgccca cccgggggcc gccatgccca ccgacaagat
     1861 gctcaccccc aacggcttcg aagcccacca cccggccatg ctcggccgcc acggggagca
     1921 gcacctcacg cccacctcgg ccggcatggt gcccatcaac ggccttcctc cgcaccatcc
     1981 ccacgcccac ctgaacgccc agggccacgg gcaactcctg ggcacagccc gggagcccaa
     2041 cccttcggtg accggcgcgc aggtcagcaa tggaagtaat tcagggcaga tggaagagat
     2101 caataccaaa gaggtggcgc agcgtatcac caccgagctc aagcgctaca gcatcccaca
     2161 ggccatcttc gcgcagaggg tgctctgccg ctcccagggg accctctcgg acctgctgcg
     2221 caaccccaaa ccctggagca aactcaaatc cggccgggag accttccgga ggatgtggaa
     2281 gtggctgcag gagccggagt tccagcgcat gtccgcgctc cgcttagcag catgcaaaag
     2341 gaaagaacaa gaacatggga aggatagagg caacacaccc aaaaagccca ggttggtctt
     2401 cacagatgtc cagcgtcgaa ctctacatgc aatattcaag gaaaataagc gtccatccaa
     2461 agaattgcaa atcaccattt cccagcagct ggggttggag ctgagcactg tcagcaactt
     2521 cttcatgaac gcaagaagga ggagtctgga caagtggcag gacgagggca gctccaattc
     2581 aggcaactca tcttcttcat caagcacttg taccaaagca ttgccaactt tcttgtacaa
     2641 agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     2701 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2761 ggacgattgg tataagcagg caatccgtta acgcgttaag tcgacaatca acctctggat
     2821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3181 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3361 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     3421 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3481 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     3721 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     3781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3901 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4021 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4081 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4141 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4201 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4261 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4321 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4381 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4441 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4501 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4561 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4621 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4681 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6721 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6781 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6841 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     6901 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     6961 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7021 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7081 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7141 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     7621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     7741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7861 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     7921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     7981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     8581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     8641 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     8701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     8761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8941 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     9001 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     9061 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     9121 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     9181 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     9241 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     9301 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     9361 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     9421 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     9481 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     9541 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
     9601 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     9661 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     9721 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     9781 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     9841 cttctaggtc ttgaaaggag tgggaatt
//
