LOCUS       sequence-350520-        5940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human HSPH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-HSPH1
FEATURES             Location/Qualifiers
     source          1..5940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(2820..3332)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3014..3035)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3226..3245
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(3359..3377)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3445..3549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3550..3618,3619..4410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3768..3787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4581..5169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5070..5089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5323..5340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5355..5494
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5420..5439)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5580..5602
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5838..5857
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat gtcggtggtg gggttggacg tgggctcgca
      181 gagctgctac atcgcggtag cccgggccgg gggcatcgag accatcgcca atgagttcag
      241 cgaccggtgc accccgtcag tcatatcatt cggatcaaaa aatagaacaa tcggagttgc
      301 agccaaaaat cagcaaatca ctcatgcaaa caatacggtg tctaacttca aaagatttca
      361 tggccgagca ttcaacgacc ccttcattca aaaggagaag gaaaacttga gttacgattt
      421 ggttccattg aaaaatggtg gagttggaat aaaggtaatg tacatgggtg aagaacatct
      481 atttagtgtg gagcagataa cagccatgtt gttgactaag ctgaaggaaa ctgctgaaaa
      541 cagcctcaag aaaccagtaa cagattgtgt tatttcagtc ccctccttct ttacagatgc
      601 tgagaggcga tctgtgttag atgctgcaca gattgttggc ctaaactgtt taagacttat
      661 gaatgacatg acagctgttg ctttgaatta cggaatttat aagcaggatc tcccaagcct
      721 ggatgagaaa cctcggatag tggtttttgt tgatatggga cattcagctt ttcaagtgtc
      781 tgcttgtgct tttaacaagg gaaaattgaa ggtactggga acagcttttg atcctttctt
      841 aggaggaaaa aacttcgatg aaaagttagt ggaacatttc tgtgcagaat ttaaaactaa
      901 gtacaagttg gatgcaaaat ccaaaatacg agcactccta cgtctgtatc aggaatgtga
      961 aaaactgaaa aagctaatga gctctaacag cacagacctt ccactgaata tcgaatgctt
     1021 tatgaatgat aaagatgttt ccggaaagat gaacaggtca caatttgaag aactctgtgc
     1081 tgaacttctg caaaagatag aagtacccct ttattcactg ttggaacaaa ctcatctcaa
     1141 agtagaagat gtgagtgcag ttgagattgt tggaggcgct acacgaattc cagctgtgaa
     1201 ggaaagaatt gccaaattct ttggaaaaga tattagcaca acactcaatg cagatgaagc
     1261 agtagccaga ggatgtgcat tacagtgtgc aatactttcc ccggcattta aagttagaga
     1321 attttccgtc acagatgcag ttccttttcc aatatctctg atctggaacc atgattcaga
     1381 agatactgaa ggtgttcatg aagtctttag tcgaaaccat gctgctcctt tctccaaagt
     1441 tctcaccttt ctgagaaggg ggccttttga gctagaagct ttctattctg atccccaagg
     1501 agttccatat ccagaagcaa aaataggccg ctttgtagtt cagaatgttt ctgcacagaa
     1561 agatggagaa aaatctagag taaaagtcaa agtgcgagtc aacacccatg gcattttcac
     1621 catctctacg gcatctatgg tggagaaagt cccaactgag gagaatgaaa tgtcttctga
     1681 agctgacatg gagtgtctga atcagagacc accagaaaac ccagacactg ataaaaatgt
     1741 ccagcaagac aacagtgaag ctggaacaca gccccaggta caaactgatg ctcaacaaac
     1801 ctcacagtct cccccttcac ctgaacttac ctcagaagaa aacaaaatcc cagatgctga
     1861 caaagcaaat gaaaaaaaag ttgaccagcc tccagaagct aaaaagccca aaataaaggt
     1921 ggtgaatgtt gagctgccta ttgaagccaa cttggtctgg cagttaggga aagaccttct
     1981 taacatgtat attgagacag agggtaagat gataatgcaa gataaattgg aaaaagaaag
     2041 gaatgatgct aaaaatgcag ttgaggaata tgtgtatgag ttcagagaca agctgtgtgg
     2101 accatatgaa aaatttatat gtgagcagga tcatcaaaat tttttgagac tcctcacaga
     2161 aactgaagac tggctgtatg aagaaggaga ggaccaagct aaacaagcat atgttgacaa
     2221 gttggaagaa ttaatgaaaa ttggcactcc agttaaagtt cggtttcagg aagctgaaga
     2281 acggccaaaa atgtttgaag aactaggaca gaggctgcag cattatgcca agatagcagc
     2341 tgacttcaga aataaggatg agaaatacaa ccatattgat gagtctgaaa tgaaaaaagt
     2401 ggagaagtct gttaatgaag tgatggaatg gatgaataat gtcatgaatg ctcaggctaa
     2461 aaagagtctt gatcaggatc cagttgtacg tgctcaggaa attaaaacaa aaatcaagga
     2521 attgaacaac acatgtgaac ccgttgtaac acaaccgaaa ccaaaaattg aatcacccaa
     2581 actggaaaga actccaaatg gcccaaatat tgataaaaag gaagaagatt tagaagacaa
     2641 aaacaatttt ggtgctgaac ctccacatca gaatggtgaa tgttacccta atgagaaaaa
     2701 ttctgttaat atggacttgg actagaaggg cgaattctgc agatatccat cacactggcg
     2761 gccgctcgag tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     2821 ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa
     2881 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2941 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3001 ggttgagttt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3061 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3121 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3181 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3241 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3301 cgccgcgctt aatgcgccgc tacagggcgc gtggggatca attcttgaag acgaaagggc
     3361 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3421 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3481 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3541 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3601 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3661 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     3721 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     3781 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     3841 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     3901 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     3961 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4021 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4081 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4141 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4201 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4261 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4321 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4381 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5341 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5401 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     5461 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     5521 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5581 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagtcgat
     5641 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     5701 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     5761 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     5821 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     5881 ccggtgatgc cggtcacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
//
