LOCUS       sequence-35053-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: pcDNA 3.2-FUS-1-373aa-V5
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    106..130
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             528..539
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cggggtttag cgactctctg gctactgaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag tttgtacaaa
      121 aaagcaggct tcgaaggaga taacaaaatg gcctcaaacg attataccca acaagcaacc
      181 caaagctatg gggcctaccc cacccagccc gggcagggct attcccagca gagcagtcag
      241 ccctacggac agcagagtta cagtggttat agccagtcca cggacacttc aggctatggc
      301 cagagcagct attcttctta tggccagagc cagaacacag gctatggaac tcagtcaact
      361 ccccagggat atggctcgac tggcggctat ggcagtagcc agagctccca atcgtcttac
      421 gggcagcagt cctcctatcc tggctatggc cagcagccag ctcccagcag cacctcggga
      481 agttacggta gcagttctca gagcagcagc tatgggcagc cccagagtgg gagctacagc
      541 cagcagcnta gctatggtgg acagcagcaa agctatggac agcagcaaag ctataatccc
      601 cctcagggct atggacagca gaaccagtac aacagcagca gtggtggtgg aggtggaggt
      661 ggaggtggag gtaactatgg ccaagatcaa tcctccatga gtagtggtgg tggcagtggt
      721 ggcggttatg gcaatcaaga ccagagtggt ggaggtggca gcggtggcta tggacagcag
      781 gaccgtggag gccgcggcag gggtggcagt ggtggcggcg gcggcggcgg cggtggtggt
      841 tacaaccgca gcagtggtgg ctatgaaccc agaggtcgtg
//
