LOCUS       sequence-350530-        4089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human DNAJB6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-DNAJB6
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(969..1481)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1163..1184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1375..1394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1508..1526)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1594..1698
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1699..1767,1768..2559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1917..1936)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2730..3318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3219..3238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3472..3489
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3504..3643
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3569..3588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3729..3751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3987..4006
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat ggtggattac tatgaagttc taggcgtgca
      181 gagacatgcc tcacccgagg atattaaaaa ggcatatcgg aaactggcac tgaagtggca
      241 tccagataaa aatcctgaga ataaagaaga agcagagaga aaattcaagc aagtagcgga
      301 ggcatatgaa gtgctgtcgg atgctaagaa acgggacatc tatgacaaat atggcaaaga
      361 aggattaaat ggtggaggag gaggtggaag tcattttgac agtccatttg aatttggctt
      421 cacattccgt aacccagatg atgtcttcag ggaatttttt ggtggaaggg acccattttc
      481 atttgacttc tttgaagacc cttttgagga cttctttggg aatcgaaggg gtccccgagg
      541 aagcagaagc cgagggacgg ggtcgttttt ctctgcgttc agtggatttc cgtcttttgg
      601 aagtggattt tcttcttttg atacaggatt tacttcattt gggtcactag gtcacggggg
      661 cctcacttca ttctcttcca cgtcatttgg tggtagtggc atgggcaact tcaaatcgat
      721 atcaacttca actaaaatgg ttaatggcag aaaaatcact acaaagagaa ttgtcgagaa
      781 cggtcaagaa agagtagaag ttgaagaaga tggccagtta aagtccttaa caataaatgg
      841 taaggagcag ctgctgcgct tggataacaa gtaaaagggc gaattctgca gatatccatc
      901 acactggcgg ccgctcgagt gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      961 ctgctgccac cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa
     1021 atatttaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1081 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1141 ccgagatagg gttgagtttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     1201 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     1261 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     1321 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     1381 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     1441 caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatcaa ttcttgaaga
     1501 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1561 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1681 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1741 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1801 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1861 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1921 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     1981 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2041 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2101 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2161 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2221 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     2281 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2341 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2401 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2461 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2521 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     2581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3421 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3481 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3541 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3601 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     3661 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3721 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3781 gcagtcgatc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     3841 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     3901 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     3961 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     4021 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     4081 ccgcgaaat
//
