LOCUS       sequence-350531-        9295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3198
FEATURES             Location/Qualifiers
     source          1..9295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2049..2073)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2081..2122
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2233..2821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2286..2306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2704..2715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2893..3126
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3204..3325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3241..3260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3295..3314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3365..3500
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3520..3539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3521..3539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3549..3566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3549..3565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3558..3580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3707..4162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3794..3813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4004..4025
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4188..4292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4293..4361,4362..5153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4511..4530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5324..5912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5813..5832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6066..6083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6200..6221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6236..6241,6242..6259,6260..6266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6274..6290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6279..6301
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6298..6314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6298..6314
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6333..6353
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6335..6353
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6381..6607
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6608..6788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6835..6960
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7453..7686
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7871..7915
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8146..8356
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8168..8364
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8215..8350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8396..8998
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8396..8415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8892..8912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9041..9158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg acatgcccaa agtcttcctg gtgaagagga ggagcctggg
     1261 ggtctcggtc cgcagctggg atgagctccc ggatgagaaa agggcagaca cctacatccc
     1321 agtgggccta ggccgcctgc tccacgaccc ccccgaggac tgccgcagcg acggcggcag
     1381 cagcagcggc agcggcagca gcagcgcggg ggagcctgga ggagcagaga gcagctcgtc
     1441 cccgcacgcc cccgagagcg aaacccccga gcccggcgac gccgagggcc ccgatggaca
     1501 cctggcgacc aagcagcgcc cggtcgccag atcgaaaatc aagttcacca caggcacgtg
     1561 cagcgactcg gtggttcaca gctgtgacct gtgtggcaag ggcttccgtc tgcagcgcat
     1621 gctgaaccgt cacctcaagt gccacaacca ggtgaaaaga cacctgtgca ccttctgcgg
     1681 caagggcttc aacgacacct tcgacctgaa gaggcacgtc cgcacacaca caggcattcg
     1741 tccctacaaa tgcaacgtct gcaataaagc cttcacccag cgctgctctc tggagtccca
     1801 cctgaagaaa atccatgggg tgcagcagca gtatgcctat aagcagcggc gggacaagct
     1861 ctacgtctgc gaggattgcg gctacacggg ccccacccag gaggacctgt acctgcacgt
     1921 gaacagtgcc catccgggca gctcgtttct caaaaagaca tctaaaaaac tggcagccct
     1981 tctgcagggc aagctgacat ccgcacacca ggagaatacc agcctgagtg aggaggagga
     2041 gaggaagtgc tcaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     2101 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     2161 ttcttggctt tatatatctt gtggaaagga cgagcaaggt ggtgtttacc tgcatcaacg
     2221 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2281 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2341 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2401 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2461 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2521 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2581 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2641 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2701 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2761 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2821 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     2881 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     2941 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3001 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3061 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3121 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3181 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3301 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3481 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3541 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3601 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3661 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3781 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3841 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3901 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3961 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4021 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4081 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4141 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4261 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4321 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4381 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4441 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4501 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4561 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4621 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4681 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4741 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4801 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4861 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4921 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4981 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5041 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5101 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5161 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5221 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5461 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5941 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6001 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6061 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6121 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6181 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6241 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6301 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6361 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6421 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6481 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6541 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6601 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6661 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6721 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6781 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6841 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6901 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6961 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7021 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7081 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7141 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7201 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7261 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7321 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7381 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7441 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7501 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7561 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7621 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7681 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7741 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     7801 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     7861 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     7921 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7981 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8041 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     8101 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8161 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8341 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     8401 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8461 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8521 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8581 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     8641 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8701 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8761 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8821 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8881 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8941 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     9001 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9061 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9181 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     9241 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
