LOCUS       sequence-350536-        4377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human DNAJB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-DNAJB4
FEATURES             Location/Qualifiers
     source          1..4377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1159..1170
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(1257..1769)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1451..1472)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1663..1682
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(1796..1814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1882..1986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1987..2055,2056..2847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2205..2224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3018..3606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3507..3526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3760..3777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3792..3931
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3857..3876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4017..4039
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4275..4294
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat ggggaaagac tattattgca ttttgggaat
      181 tgagaaagga gcttcagatg aagatattaa aaaggcttac cgaaaacaag ccctcaaatt
      241 tcatccggac aagaacaaat ctcctcaggc agaggaaaaa tttaaagagg tcgcagaagc
      301 ttatgaagta ttgagtgatc ctaaaaagag agaaatatat gatcagtttg gggaggaagg
      361 gttgaaagga ggagcaggag gtactgatgg acaaggaggt accttccggt acacctttca
      421 tggcgatcct catgctacat ttgctgcatt tttcggaggg tccaacccct ttgaaatttt
      481 ctttggaaga cgaatgggtg gtggtagaga ttctgaagaa atggaaatag atggtgatcc
      541 ttttagtgcc tttggtttca gcatgaatgg atatccaaga gacaggaatt ctgtggggcc
      601 atcccgcctc aaacaagatc ctccagttat tcatgaactt agagtatcac ttgaagagat
      661 atatagtggt tgtaccaaac ggatgaagat ttctcgaaaa aggctaaacg ctgatggaag
      721 gagttacaga tctgaggaca aaattcttac cattgagatt aaaaaagggt ggaaagaagg
      781 caccaagatt acttttccaa gagaaggaga tgaaacacca aatagtattc cagcagacat
      841 tgtttttatc attaaagaca aagatcatcc aaaatttaaa agggatggat caaatataat
      901 ttatactgct aaaattagtt tacgagaggc attgtgtggc tgctcaatta atgtaccaac
      961 actggatgga agaaacatac ctatgtcagt aaatgatatt gtgaaacccg ggatgaggag
     1021 aagaattatt ggatatgggc tgccatttcc aaaaaatcct gaccaacgtg gtgaccttct
     1081 aatagaattt gaggtgtcct tcccagatac tatatcttct tcatccaaag aagtacttag
     1141 gaaacatctt cctgcctcat agaagggcga attctgcaga tatccatcac actggcggcc
     1201 gctcgagtga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccc
     1261 cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg
     1321 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1381 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1441 tgagtttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     1501 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1561 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     1621 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     1681 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     1741 cgcgcttaat gcgccgctac agggcgcgtg gggatcaatt cttgaagacg aaagggcctc
     1801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2221 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2521 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3781 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3841 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     3901 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     3961 gccctgacgg gcttttctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agtcgatcga
     4081 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     4141 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     4201 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     4261 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     4321 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
//
