LOCUS       sequence-350537-       10621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3199
FEATURES             Location/Qualifiers
     source          1..10621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3375..3399)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3407..3448
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3559..4147
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3612..3632)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4030..4041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4219..4452
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4530..4651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4567..4586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4621..4640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4691..4826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4846..4865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4847..4865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4875..4892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4875..4891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4884..4906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5033..5488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5514..5618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5619..5687,5688..6479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5837..5856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6650..7238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7139..7158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7392..7409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7526..7547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7562..7567,7568..7585,7586..7592)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7600..7616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7605..7627
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7624..7640
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7624..7640
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7659..7679
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7661..7679
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7707..7933
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7934..8114
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8161..8286
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8779..9012
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9197..9241
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9472..9682
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9494..9690
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9541..9676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9722..10324
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9722..9741)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10218..10238
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10367..10484
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgcc aagcaccagc tttccagtcc cttccaagtt
     1261 tccacttggc cctgcggctg cggtcttcgg gagaggagaa actttggggc ccgcgccgcg
     1321 cgccggcggc accatgaagt cagcggagga agaacactat ggctatgcat cctccaacgt
     1381 cagccccgcc ctgccgctcc ccacggcgca ctccaccctg ccggccccgt gccacaacct
     1441 tcagacctcc acaccgggca tcatcccgcc ggcggatcac ccctcggggt acggagcagc
     1501 tttggacggt gggcccgcgg gctacttcct ctcctccggc cacaccaggc ctgatggggc
     1561 ccctgccctg gagagtcctc gcatcgagat aacctcgtgc ttgggcctgt accacaacaa
     1621 taaccagttt ttccacgatg tggaggtgga agacgtcctc cctagctcca aacggtcccc
     1681 ctccacggcc acgctgagtc tgcccagcct ggaggcctac agagacccct cgtgcctgag
     1741 cccggccagc agcctgtcct cccggagctg caactcagag gcctcctcct acgagtccaa
     1801 ctactcgtac ccgtacgcgt ccccccagac gtcgccatgg cagtctccct gcgtgtctcc
     1861 caagaccacg gaccccgagg agggctttcc ccgcgggctg ggggcctgca cactgctggg
     1921 ttccccgcgg cactccccct ccacctcgcc ccgcgccagc gtcactgagg agagctggct
     1981 gggtgcccgc tcctccagac ccgcgtcccc gtgcaacaag aggaagtaca gcctcaacgg
     2041 ccggcagccg ccctactcac cccaccactc gcccacgccg tccccgcacg gctccccgcg
     2101 ggtcagcgtg accgacgact cgtggttggg caacaccacc cagtacacca gctcggccat
     2161 cgtggccgcc atcaacgcgc tgaccaccga cagcagcctg gacctgggag atggcgtccc
     2221 tgtcaagtcc cgcaagacca ccctggagca gccgccctca gtggcgctca aggtggagcc
     2281 cgtcggggag gacctgggca gccccccgcc cccggccgac ttcgcgcccg aagactactc
     2341 ctctttccag cacatcagga agggcggctt ctgcgaccag tacctggcgg tgccgcagca
     2401 cccctaccag tgggcgaagc ccaagcccct gtcccctacg tcctacatga gcccgaccct
     2461 gcccgccctg gactggcagc tgccgtccca ctcaggcccg tatgagcttc ggattgaggt
     2521 gcagcccaag tcccaccacc gagcccacta cgagacggag ggcagccggg gggccgtgaa
     2581 ggcgtcggcc ggaggacacc ccatcgtgca gctgcatggc tacttggaga atgagccgct
     2641 gatgctgcag cttttcattg ggacggcgga cgaccgcctg ctgcgcccgc acgccttcta
     2701 ccaggtgcac cgcatcacag ggaagaccgt gtccaccacc agccacgagg ccatcctctc
     2761 caacaccaaa gtcctggaga tcccactcct gccggagaac agcatgcgag ccgtcattga
     2821 ctgtgccgga atcctgaaac tcagaaactc cgacattgaa cttcggaaag gagagacgga
     2881 catcgggagg aagaacacac gggtacggct ggtgttccgc gttcacgtcc cgcaacccag
     2941 cggccgcacg ctgtccctgc aggtggcctc caaccccatc gaatgctccc agcgctcagc
     3001 tcaggagctg cctctggtgg agaagcagag cacggacagc tatccggtcg tgggcgggaa
     3061 gaagatggtc ctgtctggcc acaacttcct gcaggactcc aaggtcattt tcgtggagaa
     3121 agccccagat ggccaccatg tctgggagat ggaagcgaaa actgaccggg acctgtgcaa
     3181 gccgaattct ctggtggttg agatcccgcc gtttcggaat cagaggataa ccagccccgt
     3241 tcacgtcagt ttctacgtct gcaacgggaa gagaaagcga agccagtacc agcgtttcac
     3301 ctaccttccc gccaacggta acgccatctt tctaaccgta agccgtgaac atgagcgcgt
     3361 ggggtgcttt ttcttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc
     3421 taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat
     3481 ttcgatttct tggctttata tatcttgtgg aaaggacgag ccgaaggagt gctttttaca
     3541 gcgacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3961 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4081 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4141 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4261 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4321 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4381 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4441 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4501 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4561 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4621 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4681 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4741 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4801 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4861 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4921 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4981 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6781 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7441 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7501 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7561 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7621 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7681 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7741 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7801 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7861 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7921 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7981 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8041 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8101 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8161 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8221 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8281 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8341 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8401 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8461 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8521 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8581 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8641 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8701 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8761 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8821 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8881 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8941 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9001 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9061 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9121 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9181 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9241 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9301 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9361 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9421 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9481 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9541 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9601 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9661 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9721 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9781 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9841 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9901 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9961 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10021 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10081 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10141 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10201 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10261 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    10321 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10381 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10441 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10501 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10561 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10621 t
//
