LOCUS       sequence-350539-       10323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding B3GALT5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: B3GALT5_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1001
                     /label=chicken beta-actin promoter
     intron          1002..2018
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1942..1964
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2026..2045
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2134..2158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3128..3152)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3172..3195
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3230..3779
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3374..3391)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3601..3620
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3782..3784,3785..3787,3788..4501)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3782..3784,3785..3787,3788..4498)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3827..3848)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4088..4107)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4435..4456
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4578..5166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4631..4651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5049..5060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5169..5185)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5170..5186)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5375..5608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5680..5801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5717..5736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5771..5790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5841..5976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5996..6015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5997..6015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6038..6396)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6078..6099)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6290..6309
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6423..6527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6528..6596,6597..7388)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6746..6765)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7559..8147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8048..8067
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8322..8625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8627..8825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8780..8800
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8843..9023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9070..9195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9692..9925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10110..10154
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggnsgcgc gccrggcggg gsggggcggg gsgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     1021 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     1081 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     1141 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     1201 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     1261 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     1321 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     1381 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1441 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1501 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1561 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1621 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1681 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1741 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1801 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1861 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1921 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1981 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     2041 ttgtgctgtc tcatcatttt ggcaaagaat tcccctagcc gcggaatcta gactgccgga
     2101 tcgaattgtc gacgaattcg gatctggcca atcacaagtt tgtacaaaaa agcaggctcc
     2161 gcggccgccc ccttcaccat ggctttcccg aagatgagat tgatgtatat ttgccttctg
     2221 gttctggggg ctctttgttt gtattttagc atgtacagtc taaatccttt caaagaacag
     2281 tcctttgttt acaagaaaga cgggaacttc cttaagctcc cagatacaga ctgcaggcag
     2341 acacctccct tcctcgtcct gctggtgacc tcatcccaca aacagttggc tgagcgcatg
     2401 gccatccggc agacgtgggg gaaagagagg acggtgaagg gaaagcagct gaagacattc
     2461 ttcctcctgg ggaccaccag cagtgcagcg gaaacaaaag aggtggacca ggagagccag
     2521 cgacacgggg acattatcca gaaggatttc ctagacgtct attacaatct gaccctgaag
     2581 accatgatgg gcatagaatg ggtccatcgc ttttgtcctc aggcggcgtt tgtgatgaaa
     2641 acagactcag acatgttcat caatgttgac tatctgactg aactgcttct gaagaaaaac
     2701 agaacaacca ggtttttcac tggcttcttg aaactcaatg agtttcccat caggcagcca
     2761 ttcagcaagt ggtttgtcag taaatctgaa tatccgtggg acaggtaccc accattctgc
     2821 tccggcaccg gctacgtgtt ttctggcgac gtggcgagtc aggtgtacaa tgtctccaag
     2881 agcgtcccat acattaaact ggaagacgtg tttgtggggc tctgcctcga aaggctgaac
     2941 atcagattgg aggagctcca ctcccagccg accttttttc cagggggctt acgcttctcc
     3001 gtatgcctct tcaggaggat cgtggcctgc cacttcatca agcctcggac tctcttggac
     3061 tactggcagg ctctagagaa ttcccggggg gaagattgtc cgcctgtctg aaagggtggg
     3121 cgcgccgacc cagctttctt gtacaaagtg gtgggaggag gtggatctgg cgactacaag
     3181 gacgacgatg acaagtgact cgagcgggat caattccgcc ccccccctaa cgttactggc
     3241 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3301 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3361 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3421 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3481 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3541 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3601 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3661 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3721 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
     3781 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3841 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3901 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3961 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4021 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4081 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4141 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4201 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4261 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4321 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4381 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4441 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4501 aagcggccgc gactctagag tcgacctgca ggcatgcaag cttcaggtag ccggctaacg
     4561 ttatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4981 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5161 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     5221 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     5281 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5341 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5401 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5581 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5641 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5701 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5761 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     5821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5941 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6001 tagtgagtcg tattagcatg tgagcaaaag gccagcataa atcaaaagaa tagaccgaga
     6061 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     6121 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     6181 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     6241 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     6301 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     6361 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     6421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6541 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6601 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6661 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6721 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6781 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6841 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6901 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6961 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7021 cgaagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7081 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7141 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7201 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7261 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7321 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7381 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7441 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7501 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7681 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8221 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     8281 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8341 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8401 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8461 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8521 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     8581 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     8641 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     8701 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     8761 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     8821 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8881 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8941 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9001 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9061 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9121 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9181 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9241 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9301 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9361 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9421 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9481 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     9541 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9601 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9661 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9721 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9781 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9841 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9901 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9961 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10021 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10081 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10141 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10201 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10261 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10321 ggg
//
