LOCUS       sequence-350540-        5562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human HSP90AA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-HSP90AA1
FEATURES             Location/Qualifiers
     source          1..5562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(2442..2954)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2636..2657)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2848..2867
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(2981..2999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3067..3171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3172..3240,3241..4032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3390..3409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4203..4791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4692..4711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4945..4962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4977..5116
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5042..5061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5202..5224
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5460..5479
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat gcctgaggaa acccagaccc aagaccaacc
      181 gatggaggag gaggaggttg agacgttcgc ctttcaggca gaaattgccc agttgatgtc
      241 attgatcatc aatactttct actcgaacaa agagatcttt ctgagagagc tcatttcaaa
      301 ttcatcagat gcattggaca aaatccggta tgaaagcttg acagatccca gtaaattaga
      361 ctctgggaaa gagctgcata ttaaccttat accgaacaaa caagatcgaa ctctcactat
      421 tgtggatact ggaattggaa tgaccaaggc tgacctgatc aataaccttg gtactatcgc
      481 caagtctggg accaaagcgt tcatggaagc tttgcaggct ggtgcagata tctctatgat
      541 tggccagttc ggtgttggtt tttattctgc ttatttggtt gctgagaaag taactgtgat
      601 caccaaacat aacgacgatg agcagtacgc ttgggagtcc tcagcagggg gatcattcac
      661 agtgaggaca gacacaggtg aacctatggg tcgtggaaca aaagttatcc tacacctgaa
      721 agaagaccaa actgagtact tggaggaacg aagaataaag gagattgtga agaaacattc
      781 tcagtttatt gggtatccca ttactctttt tgtggagaag gaacgtgata aagaagtaag
      841 cgatgatgag gctgaagaaa aggaagacaa agaagaagaa aaagaaaaag aagagaaaga
      901 gtcggaagac aaacctgaaa ttgaagatgt tggttctgat gaggaagaag aaaagaagga
      961 tggtgacaag aagaagaaga agaagattaa ggaaaagtac atcgatcaag aagagctcaa
     1021 caaaacaaag cccatctgga ccagaaatcc cgacgatatt actaatgagg agtacggaga
     1081 attctataag agcttgacca atgactggga agatcacttg gcagtgaagc atttttcagt
     1141 tgaaggacag ttggaattca gagcccttct atttgtccca cgacgtgctc cttttgatct
     1201 gtttgaaaac agaaagaaaa agaacaacat caaattgtat gtacgcagag ttttcatcat
     1261 ggataactgt gaggagctaa tccctgaata tctgaacttc attagagggg tggtagactc
     1321 ggaggatctc cctctaaaca tatcccgtga gatgttgcaa caaagcaaaa ttttgaaagt
     1381 tatcaggaag aatttggtca aaaaatgctt agaactcttt actgaactgg cggaagataa
     1441 agagaactac aagaaattct atgagcagtt ctctaaaaac ataaagcttg gaatacacga
     1501 agactctcaa aatcggaaga agctttcaga gctgttaagg tactacacat ctgcctctgg
     1561 tgatgagatg gtttctctca aggactactg caccagaatg aaggagaacc agaaacatat
     1621 ctattatatc acaggtgaga ccaaggacca ggtagctaac tcagcctttg tggaacgtct
     1681 tcggaaacat ggcttagaag tgatctatat gattgagccc attgatgagt actgtgtcca
     1741 acagctgaag gaatttgagg ggaagacttt agtgtcagtc accaaagaag gcctggaact
     1801 tccagaggat gaagaagaga aaaagaagca ggaagagaaa aaaacaaagt ttgagaacct
     1861 ctgcaaaatc atgaaagaca tattggagaa aaaagttgaa aaggtggttg tgtcaaaccg
     1921 attggtgaca tccccatgct gtattgtcac aagcacatat ggctggacag caaacatgga
     1981 gagaatcatg aaagctcaag ccctaagaga caactcaaca atgggttaca tggcagcaaa
     2041 gaaacacctg gagataaacc ctgaccattc cattattgag accttaaggc aaaaggcaga
     2101 ggctgataag aacgacaagt ctgtgaagga tctggtcatc ttgctttatg aaactgcgct
     2161 cctgtcttct ggcttcagtc tggaagatcc ccagacacat gctaacagga tctacaggat
     2221 gatcaaactt ggtctgggta ttgatgaaga tgaccctact gctgatgata ccagtgctgc
     2281 tgtaactgaa gaaatgccac cccttgaagg agatgacgac acatcacgca tggaagaagt
     2341 agactaaaag ggcgaattct gcagatatcc atcacactgg cggccgctcg agtgatccgg
     2401 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccccggtt gataatcaga
     2461 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaagc gttaatattt
     2521 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     2581 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt ttgttccagt
     2641 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2701 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     2761 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     2821 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     2881 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     2941 gctacagggc gcgtggggat caattcttga agacgaaagg gcctcgtgat acgcctattt
     3001 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     3421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     3721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4321 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4981 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     5041 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     5101 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5161 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5221 gaggttttca ccgtcatcac cgaaacgcgc gaggcagtcg atcgacgctc tcccttatgc
     5281 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     5341 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     5401 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     5461 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     5521 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
//
