LOCUS       sequence-350542-       10059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding MIS18A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: MIS18A_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        725..1001
                     /label=chicken beta-actin promoter
     intron          1002..2018
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1942..1964
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2026..2045
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2134..2158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2864..2888)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2908..2931
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2966..3515
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3110..3127)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3337..3356
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3518..3520,3521..3523,3524..4237)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3518..3520,3521..3523,3524..4234)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3563..3584)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3824..3843)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4171..4192
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4314..4902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4367..4387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4785..4796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4905..4921)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4906..4922)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5111..5344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5416..5537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5453..5472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5507..5526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5577..5712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5732..5751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5733..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5774..6132)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5814..5835)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6026..6045
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6159..6263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6264..6332,6333..7124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6482..6501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7295..7883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7784..7803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        8058..8361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8363..8561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8516..8536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8579..8759
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8806..8931
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9428..9661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9846..9890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
      781 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
      841 ggggnsgcgc gccrggsggg gsggggsggg gsgrggggsg gggsggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     1021 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     1081 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     1141 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     1201 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     1261 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     1321 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     1381 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1441 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1501 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1561 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1621 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1681 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1741 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1801 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1861 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1921 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1981 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     2041 ttgtgctgtc tcatcatttt ggcaaagaat tcccctagcc gcggaatcta gactgccgga
     2101 tcgaattgtc gacgaattcg gatctggcca atcacaagtt tgtacaaaaa agcaggcacc
     2161 atggcaggcg ttcggtcact gaggtgtagc agaggatgcg ctggcggctg tgagtgcggc
     2221 gacaagggca aatgcagcga ctcctcgctg ttgggcaaga gactctccga agactcgagc
     2281 cgccaccagc tgttgcagaa gtgggcgagc atgtggagct ccatgagcga agacgcgtcg
     2341 gtggccgaca tggagagggc gcagctggag gaggaggcgg cggctgcgga ggagaggccg
     2401 ctggtgttcc tgtgctccgg ctgccggcgg ccgctgggcg actcgctgag ctgggtggcc
     2461 agccaggagg acaccaactg catcctgctt cgctgtgttt cctgtaatgt ttctgtggat
     2521 aaggaacaga agctatccaa acgtgaaaag gaaaatggtt gcgtccttga gactttgtgc
     2581 tgcgcggggt gctcactcaa tcttggctac gtgtacagat gcacgcccaa gaatcttgat
     2641 tacaagagag acttgttttg cctcagtgtt gaagccattg aaagttatgt tttagggtcc
     2701 tctgaaaagc aaattgtgtc agaagataaa gagcttttta atcttgaaag cagagttgaa
     2761 atagaaaagt ctctaacaca gatggaagat gtcttgaaag cattacaaat gaagctgtgg
     2821 gaggccgaat ccaaattgtc ctttgccact tgtaaaagct agaacccagc tttcttgtac
     2881 aaagtggtgg gaggaggtgg atctggcgac tacaaggacg acgatgacaa gtgactcgag
     2941 cgggatcaat tccgcccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3001 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3061 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3121 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3181 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3241 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3301 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3361 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3421 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3481 ggggacgtgg ttttcctttg aaaaacacga taataccatg gtgagcaagg gcgaggagct
     3541 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3601 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3661 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3721 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3781 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3841 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3901 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3961 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4021 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4081 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     4141 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4201 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcga
     4261 cctgcaggca tgcaagcttc aggtagccgg ctaacgttat caagcttatc gataatcaac
     4321 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4381 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4441 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4501 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4561 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4621 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4681 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4741 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4801 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4861 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4921 gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4981 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5041 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5101 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5341 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5401 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5521 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5701 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt agcatgtgag
     5761 caaaaggcca gcataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5821 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5881 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5941 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6001 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6061 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6121 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6181 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6241 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6301 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6361 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6421 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6481 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6541 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6601 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6661 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6721 tgggggatca tgtaactcgc cttgatcgtt gggaaccgaa gctgaatgaa gccataccaa
     6781 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6841 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6901 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6961 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7021 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7081 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7141 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7201 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7441 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7981 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     8041 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8101 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8161 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8221 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8281 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8341 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8401 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8461 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8521 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     8581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8761 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8821 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8881 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8941 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9001 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9061 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9121 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9181 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9241 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     9301 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9361 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9421 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9481 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9541 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9601 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9661 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9721 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9781 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9841 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9901 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     9961 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    10021 caccattatc gtttcagacc cacctcccaa ccccgaggg
//
