LOCUS       sequence-350544-        9769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3201
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2523..2547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2555..2596
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2707..3295
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2760..2780)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3178..3189)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3367..3600
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3678..3799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3715..3734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3769..3788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3994..4013)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3995..4013)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4023..4040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4023..4039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4032..4054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4181..4636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4268..4287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4478..4499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4662..4766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4767..4835,4836..5627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4985..5004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5798..6386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6287..6306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6540..6557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6674..6695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6710..6715,6716..6733,6734..6740)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6748..6764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6753..6775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6772..6788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6772..6788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6807..6827
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6809..6827
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6855..7081
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7082..7262
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7309..7434
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7927..8160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8345..8389
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8620..8830
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8642..8838
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8689..8824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8870..9472
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8870..8889)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9366..9386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9515..9632
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgcc ccattctcct ctgatctcca ttcctcatgt
     1261 gtggtgtcac ccagaagagg aggaaagaat gcatgatgaa cttctacaag cagtatccaa
     1321 ggggccggtg atgttcaggg atgtttccat agacttctct caagaggaat gggaatgcct
     1381 ggacgctgat cagatgaatt tatacaaaga agtgatgttg gagaatttca gcaacctggt
     1441 ttcagtggga ctttccaatt ctaagccagc tgtgatctcc ttattggaac aaggaaaaga
     1501 gccctggatg gttgatagag agctgactag aggcctgtgt tcagatctgg aatcaatgtg
     1561 tgagaccaaa atattatctc taaagaagag acatttcagt caagtaataa ttacccgtga
     1621 agacatgtct acttttattc agcccacatt tcttattcca cctcaaaaaa ctatgagtga
     1681 agagaaacca tgggaatgta agatatgtgg aaagaccttt aatcaaaact cacaatttat
     1741 ccaacatcag agaattcatt ttggtgaaaa acactatgaa tctaaggagt atgggaagtc
     1801 ctttagtcgt ggctcactcg ttactcgaca tcagaggatt cacactggta aaaaacccta
     1861 tgaatgtaag gaatgtggca aggcttttag ttgtagttca tatttttctc aacatcagag
     1921 gattcacact ggtgagaaac cctatgaatg taaggaatgt ggaaaagcct ttaagtattg
     1981 ctcaaacctt aatgatcatc agagaattca cactggtgag aaaccctatg aatgtaaagt
     2041 atgtggaaaa gcctttacta aaagttcaca actttttcta catctgagaa ttcatactgg
     2101 tgagaaacct tatgaatgta aagaatgtgg gaaagccttt actcaacact caaggcttat
     2161 tcagcatcag agaatgcata ctggtgagaa accttatgaa tgtaagcagt gtgggaaggc
     2221 ctttaatagt gcctcaacac ttactaacca tcacagaatt catgctggtg agaagctcta
     2281 tgaatgtgaa gaatgtagaa aggcctttat tcagagctca gaacttattc aacatcagag
     2341 aatccataca gatgaaaaac catatgaatg taatgaatgt gggaaggcct ttaataaagg
     2401 ctcaaatctt actcgacatc agagaattca cactggtgag aaaccctatg actgtaagga
     2461 atgtggaaag gcttttggta gtcgctctga cctcattcgc catgagggaa ttcatactgg
     2521 tttgccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     2581 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     2641 gctttatata tcttgtggaa aggacgacct agtttaatct gaaagactgc gacgcgttaa
     2701 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2761 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2821 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2881 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2941 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3001 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3061 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3121 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3181 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3241 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3301 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3361 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3421 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3481 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3541 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3601 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3661 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3721 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3781 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4021 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4081 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4141 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4201 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4261 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4321 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4381 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4441 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4501 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4561 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4621 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4681 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4741 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4801 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4861 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4921 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4981 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5041 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5101 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5161 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5221 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5281 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5341 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5401 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5461 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5521 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5581 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5641 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5701 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5761 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5821 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5881 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5941 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6001 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6061 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6121 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6181 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6241 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6301 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6361 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6421 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6481 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6541 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6601 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6661 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6721 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6781 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6841 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6901 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6961 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7021 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7081 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7141 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7201 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7261 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7321 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7381 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7441 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7501 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7561 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7621 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7681 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7741 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7801 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7861 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7921 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7981 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8041 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8101 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8161 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8221 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8281 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8341 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8401 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8461 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8521 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     8581 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8641 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8761 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8821 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     8881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9061 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     9121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     9481 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9541 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9601 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9661 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9721 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
