LOCUS       sequence-350547-        5289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human HSPA1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-HSPA1L
FEATURES             Location/Qualifiers
     source          1..5289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(2169..2681)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2363..2384)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2575..2594
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(2708..2726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2794..2898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2899..2967,2968..3759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3117..3136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3930..4518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4419..4438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4672..4689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4704..4843
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4769..4788)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4929..4951
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5187..5206
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat ggctactgcc aagggaatcg ccataggcat
      181 cgacctgggc accacctact cctgtgtggg ggtgttccag cacggcaagg tggagatcat
      241 cgccaacgac cagggcaacc gcaccacccc cagctacgtg gccttcacag acaccgagcg
      301 gctcattggg gatgcggcca agaaccaggt agcaatgaac ccccagaaca ctgtttttga
      361 tgctaaacgt ctgatcggca ggaaatttaa tgatcctgtt gtacaagcag atatgaaact
      421 ttggcctttt caagtgatta atgaaggagg caagcccaaa gtccttgtgt cctacaaagg
      481 ggagaataaa gctttctacc ctgaggaaat ctcttcgatg gtattgacta agttgaagga
      541 gactgctgag gcctttttgg gccaccctgt caccaatgca gtgattaccg tgccagccta
      601 tttcaatgac tctcaacgtc aggctactaa ggatgcaggt gtgattgctg gacttaacgt
      661 gctaagaatc atcaatgagc ccacggctgc tgccattgcc tatggtttag ataaaggagg
      721 tcagggagaa cgacatgtcc tgatttttga tctgggtgga ggcacatttg atgtgtcaat
      781 tctgaccata gatgatggga tttttgaggt aaaggccact gctggggaca ctcacctggg
      841 cggggaggac tttgacaaca ggcttgtgag ccacttcgtg gaggagttca agaggaaaca
      901 caaaaaggac atcagccaga acaagcgagc cgtgaggcgg ctgcgcaccg cctgcgagag
      961 ggccaagagg accctgtcgt ccagcaccca ggccaaccta gaaattgatt cactttatga
     1021 aggcattgac ttctatacat ccatcaccag agctcgattt gaagagttgt gtgcagacct
     1081 gtttaggggt accttggagc ctgtagaaaa agcgcttcgg gatgccaaga tggataaggc
     1141 taaaatccat gacattgttt tagtaggggg ctccacccgc atccccaagg tgcagcggct
     1201 gcttcaggac tacttcaatg gacgtgatct caacaagagc atcaaccctg atgaggccgt
     1261 agcatatggg gctgcggtac aagcagccat cctgatgggg gacaagtctg agaaggtaca
     1321 ggacctgctg ctgctggacg tggctcccct gtccctgggg ctggagacgg ctgggggcgt
     1381 gatgactgcc ctgataaagc gcaactccac catccccacc aagcagacac agatcttcac
     1441 cacctactct gacaaccaac ccggggtgct gatccaggtg tatgagggcg agagggccat
     1501 gacaaaggac aacaacctgc tggggcggtt tgacctgact ggaatccctc cagcacccag
     1561 gggagttcct cagatcgagg tgacgtttga cattgatgcc aatggtattc tcaatgtcac
     1621 agccacggac aagagcaccg gcaaggtgaa caagatcacc atcaccaatg acaagggccg
     1681 cctgagcaag gaggagattg agcgcatggt tctggatgct gagaaatata aagctgaaga
     1741 tgaggtccag agggagaaaa ttgctgcaaa gaatgcctta gaatcctatg cttttaacat
     1801 gaagagtgtc gtgagtgatg aaggtttgaa gggcaagatt agtgagtctg ataaaaataa
     1861 aatattggat aaatgcaacg agctcctttc gtggctggag gtcaatcaac tggcagagaa
     1921 agatgagttt gatcataaga gaaaggaatt ggagcagatg tgtaacccta tcatcacaaa
     1981 actctaccaa ggaggatgca ctgggcctgc ctgcggaaca gggtatgtgc ctggaaggcc
     2041 tgccacaggc cccacaattg aagaagtaga ttaaaagggc gaattctgca gatatccatc
     2101 acactggcgg ccgctcgagt gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     2161 ctgctgccac cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa
     2221 atatttaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2281 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2341 ccgagatagg gttgagtttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2401 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2461 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2521 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2581 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2641 caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatcaa ttcttgaaga
     2701 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     2761 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3121 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     3181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3421 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     3481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4681 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4741 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4801 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     4861 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4921 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4981 gcagtcgatc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     5041 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5101 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5161 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5221 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     5281 ccgcgaaat
//
