LOCUS       sequence-350548-       10190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding CBR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: CBR1_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          160..1176
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1100..1122
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1184..1203
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1292..1316
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2154..2178)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2198..2221
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2256..2805
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2400..2417)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2627..2646
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2808..2810,2811..2813,2814..3527)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2808..2810,2811..2813,2814..3524)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2853..2874)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3114..3133)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3461..3482
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3604..4192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3657..3677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4075..4086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4195..4211)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4196..4212)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4401..4634
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4706..4827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4743..4762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4797..4816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4867..5002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5022..5041)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5023..5041)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5064..5422)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5104..5125)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5316..5335
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5449..5553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5554..5622,5623..6414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5772..5791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6585..7173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7074..7093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7348..7651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7653..7851
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7806..7826
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7869..8049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8096..8221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8718..8951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9136..9180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9478..9595
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9693..10072
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10074..10190,1..159)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
       61 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      181 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      241 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      301 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      361 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      421 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      481 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      541 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
      601 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
      661 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
      721 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      781 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      841 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      901 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      961 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1021 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1081 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1141 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1201 gtgctgtctc atcattttgg caaagaattc ccctagccgc ggaatctaga ctgccggatc
     1261 gaattgtcga cgaattcgga tctggccaat cacaagtttg tacaaaaaag caggcaccat
     1321 gtcgtccggc atccatgtag cgctggtgac tggaggcaac aagggcatcg gcttggccat
     1381 cgtgcgcgac ctgtgccggc tgttctcggg ggacgtggtg ctcacggcgc gggacgtgac
     1441 gcggggccag gcggccgtac agcagctgca ggcggagggc ctgagcccgc gcttccacca
     1501 gctggacatc gacgatctgc agagcatccg cgccctgcgc gacttcctgc gcaaggagta
     1561 cgggggcctg gacgtgctgg tcaacaacgc gggcatcgcc ttcaaggttg ctgatcccac
     1621 accctttcat attcaagctg aagtgacgat gaaaacaaat ttctttggta cccgagatgt
     1681 gtgcacagaa ttactccctc taataaaacc ccaagggaga gtggtgaacg tatctagcat
     1741 catgagcgtc agagccctta aaagctgcag cccagagctg cagcagaagt tccgcagtga
     1801 gaccatcact gaggaggagc tggtggggct catgaacaag tttgtggagg atacaaagaa
     1861 gggagtgcac cagaaggagg gctggcccag cagcgcatac ggggtgacga agattggcgt
     1921 caccgttctg tccaggatcc acgccaggaa actgagtgag cagaggaaag gggacaagat
     1981 cctcctgaat gcctgctgcc cagggtgggt gagaactgac atggcgggac ccaaggccac
     2041 caagagccca gaagaaggtg cagagacccc tgtgtacttg gcccttttgc ccccagatgc
     2101 tgagggtccc catggacaat ttgtttcaga gaagagagtt gaacagtggt agaacccagc
     2161 tttcttgtac aaagtggtgg gaggaggtgg atctggcgac tacaaggacg acgatgacaa
     2221 gtgactcgag cgggatcaat tccgcccccc ccctaacgtt actggccgaa gccgcttgga
     2281 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2341 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2401 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2461 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2521 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2581 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     2641 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     2701 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     2761 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtgagcaagg
     2821 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2881 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2941 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3001 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3061 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3121 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3181 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3241 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3301 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3361 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3421 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3481 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     3541 ctagagtcga cctgcaggca tgcaagcttc aggtagccgg ctaacgttat caagcttatc
     3601 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3661 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3721 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3781 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3841 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3901 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3961 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4021 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4081 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4141 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4201 cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     4261 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     4321 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     4381 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     4441 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4501 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4561 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4621 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     4681 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     4741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4801 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     4861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4921 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4981 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     5041 agcatgtgag caaaaggcca gcataaatca aaagaataga ccgagatagg gttgagtgtt
     5101 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5161 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5221 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5281 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5341 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5401 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5461 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5521 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5581 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5641 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5701 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5761 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5821 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5881 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5941 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6001 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgaa gctgaatgaa
     6061 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6121 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6181 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6241 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6301 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6361 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7201 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7261 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     7321 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     7381 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     7441 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     7501 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     7561 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     7621 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     7681 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     7741 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     7801 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     7861 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8041 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8101 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8161 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8221 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8281 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8341 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8401 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8461 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8521 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     8581 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8641 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8701 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     8761 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     8821 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     8881 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8941 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9001 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9061 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9121 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9181 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9241 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9301 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9361 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     9421 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     9481 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9541 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9601 ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct agccctagat
     9661 ttaaatcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     9721 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9781 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9841 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9901 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     9961 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10021 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
    10081 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
    10141 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
//
