LOCUS       sequence-350549-        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human BAG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-BAG2
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     362..381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     615..632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    647..786
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(712..731)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     872..894
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1130..1149
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1233..1252
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1233..1251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1283..1305
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1324..1341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1351..1368
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(2111..2623)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2305..2326)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2517..2536
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(2650..2668)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2736..2840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2841..2909,2910..3701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3059..3078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3872..3999,1..461)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
       61 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      541 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      601 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
      661 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
      721 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
      781 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      841 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      901 ccgtcatcac cgaaacgcgc gaggcagtcg atcgacgctc tcccttatgc gactcctgca
      961 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     1021 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     1081 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     1141 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     1201 cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca
     1261 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac catgggcagc
     1321 agccatcatc atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgactagt
     1381 atggctcagg cgaagatcaa cgctaaagcc aacgaggggc gcttctgccg ctcctcctcc
     1441 atggctgacc gctccagccg cctgctggag agcctggacc agctggagct cagggttgaa
     1501 gctttgagag aagcagcaac tgctgttgag caagagaaag aaatccttct ggaaatgatc
     1561 cacagtatcc aaaatagcca ggacatgagg cagatcagtg acggagaaag agaagaatta
     1621 aatctgactg caaaccgttt gatgggaaga actctcaccg ttgaagtgtc agtagaaaca
     1681 attagaaacc cccagcagca agaatcccta aagcatgcca caaggattat tgatgaggtg
     1741 gtcaataagt ttctggatga tttgggaaat gccaagagtc atttaatgtc gctctacagt
     1801 gcatgttcat ctgaggtgcc acatgggcca gttgatcaga agtttcaatc catagtaatt
     1861 ggctgtgctc ttgaagatca gaagaaaatt aagagaagat tagagactct gcttagaaat
     1921 attgaaaact ctgacaaggc catcaagcta ttagagcatt ctaaaggagc tggttccaaa
     1981 actctgcaac aaaatgctga aagcagattc aattagaagg gcgaattctg cagatatcca
     2041 tcacactggc ggctgctcga gtgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     2101 ggctgctgcc accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc
     2161 aaatatttaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     2221 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     2281 gaccgagata gggttgagtt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     2341 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     2401 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     2461 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     2521 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     2581 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtggggatc aattcttgaa
     2641 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2701 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2761 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2821 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2881 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2941 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3001 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3061 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     3121 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3181 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3241 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3301 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3361 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     3421 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3481 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3541 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3601 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3661 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3961 taccaactct ttttccgaag gtaactggct tcagcagag
//
