LOCUS       sequence-35055-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: pcDNA 3.2-FUS-1-413aa-V5
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             517..528
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctt
      121 cgaaggagat aacaaaatgg cctcaaacga ttatacccaa caagcaaccc aaagctatgg
      181 ggcctacccc acccagcccg ggcagggcta ttcccagcag agcagtcagc cctacggaca
      241 gcagagttac agtggttata gccagtccac ggacacttca ggctatggcc agagcagcta
      301 ttcttcttat ggccagagcc agaacacagg ctatggaact cagtcaactc cccagggata
      361 tggctcgact ggcggctatg gcagtagcca gagctcccaa tcgtcttacg ggcagcagtc
      421 ctcctatcct ggctatggcc agcagccagc tcccagcagc acctcgggaa gttacggtag
      481 cagttctcag agcagcagct atgggcagcc ccagagtggg agctacagcc agcagcctag
      541 ctatggtgga cagcagcaaa gctatggaca gcagcaaagc tataatcccc ctcagggcta
      601 tggacagcag aaccagtaca acagcagcag tggtggtgga ggtggaggtg gaggtggagg
      661 taactatggc caagatcaat cctccatgag tagtggtggt ggcagtggtg gcggttatgg
      721 caatcaagac cagagtggtg gaggtggcag cggtggctat ggacagcagg accgtggagg
      781 ccgcggcagg ggtggcagtg gtggcggcgg cggcggcggc ggtggtggtt acaaccgcag
      841 cagtggtggc tatgaaccca gaggtc
//
