LOCUS       sequence-350668-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Flag-PolB(T304I) and a puromycin 
            resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Andrews J, Sharma N, Wilk A, Clark J, Slyskova J, Koczor CA,
            Lans H, Prakash A, Sobol RW
  TITLE     Stability and sub-cellular localization of DNA polymerase beta is 
            regulated by interactions with NQO1 and XRCC1 in response to 
            oxidative stress.
  JOURNAL   Nucleic Acids Res. 2019 Jul 9;47(12):6269-6286. doi: 
            10.1093/nar/gkz293.
  PUBMED    31287140
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-9- 9"; volume: "47"; issue: 
            "12"; pages: "6269-6286"
COMMENT     Sequence Label: pLVX-Flag-PolB(T304I)-Puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             14..647
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    694..819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1316..1549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1734..1778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2041..2156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2214..2517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2518..2721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2672..2692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2718..2742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2837..2861
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2885..2908
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3936..3960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3992..4566,4567..4569,4570..4578)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4159..4176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4386..4405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4598..5197
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4598..4617)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5094..5114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5211..5799
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5264..5284)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5682..5693)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6006..6639
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6767..6783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6767..6783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6780..6802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6791..6807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6815..6821,6822..6839,6840..6845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6860..6881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6998..7015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7169..7757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7249..7268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7928..8719,8720..8788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8551..8570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8789..8893)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8941..9075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8978..8997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9032..9051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
       61 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
      121 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
      181 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
      241 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
      301 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
      361 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
      421 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
      481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      661 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      721 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      781 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      841 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
      901 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
      961 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1021 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1081 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1141 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1201 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1261 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1321 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1381 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1441 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1501 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1561 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1621 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1681 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1741 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1801 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1861 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1921 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1981 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     2041 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2101 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2161 gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca
     2221 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2281 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2341 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2401 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2461 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2521 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2581 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2641 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2701 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2761 cgctgttttg acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt
     2821 cctcgagact agatcaacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
     2881 catggactac aaagacgatg acgataaagg catgagcaaa cggaaggcgc cgcaggagac
     2941 tctcaacggg ggaatcaccg acatgctcac agaactcgca aactttgaga agaacgtgag
     3001 ccaagctatc cacaagtaca atgcttacag aaaagcagca tctgttatag caaaataccc
     3061 acacaaaata aagagtggag ctgaagctaa gaaattgcct ggagtaggaa caaaaattgc
     3121 tgaaaagatt gatgagtttt tagcaactgg aaaattacgt aaactggaaa agattcggca
     3181 ggatgatacg agttcatcca tcaatttcct gactcgagtt agtggcattg gtccatctgc
     3241 tgcaaggaag tttgtagatg aaggaattaa aacactagaa gatctcagaa aaaatgaaga
     3301 taaattgaac catcatcagc gaattgggct gaaatatttt ggggactttg aaaaaagaat
     3361 tcctcgtgaa gagatgttac aaatgcaaga tattgtacta aatgaagtta aaaaagtgga
     3421 ttctgaatac attgctacag tctgtggcag tttcagaaga ggtgcagagt ccagtggtga
     3481 catggatgtt ctcctgaccc atcccagctt cacttcagaa tcaaccaaac agccaaaact
     3541 gttacatcag gttgtggagc agttacaaaa ggttcatttt atcacagata ccctgtcaaa
     3601 gggtgagaca aagttcatgg gtgtttgcca gcttcccagt aaaaatgatg aaaaagaata
     3661 tccacacaga agaattgata tcaggttgat acccaaagat cagtattact gtggtgttct
     3721 ctatttcact gggagtgata ttttcaataa gaatatgagg gctcatgccc tagaaaaggg
     3781 tttcacaatc aatgagtaca ccatccgtcc cttgggagtc attggagttg caggagaacc
     3841 cctgccagtg gatagtgaaa aagacatctt tgattacatc cagtggaaat accgggaacc
     3901 caaggaccgg agcgaatgaa agggtgggcg cgccgaccca gctttcttgt acaaagtggt
     3961 tgatctagtt ctagagcggc cgcggatccc gcccctctcc ctcccccccc cctaacgtta
     4021 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4081 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4141 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4201 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4261 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4321 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4381 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4441 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4501 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4561 gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt
     4621 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     4681 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     4741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4801 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4921 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5161 gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac aatcaacctc
     5221 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5281 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5341 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5401 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5461 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5521 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5581 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5641 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5701 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5761 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     5821 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5881 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5941 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     6001 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6061 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     6121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     6181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     6241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     6301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     6361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     6421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     6481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6661 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6721 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6961 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7801 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9061 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9121 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9181 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtacta
//
