LOCUS       sequence-350669-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin 
            resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Andrews J, Sharma N, Wilk A, Clark J, Slyskova J, Koczor CA,
            Lans H, Prakash A, Sobol RW
  TITLE     Stability and sub-cellular localization of DNA polymerase beta is 
            regulated by interactions with NQO1 and XRCC1 in response to 
            oxidative stress.
  JOURNAL   Nucleic Acids Res. 2019 Jul 9;47(12):6269-6286. doi: 
            10.1093/nar/gkz293.
  PUBMED    31287140
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-9- 9"; volume: "47"; issue: 
            "12"; pages: "6269-6286"
COMMENT     Sequence Label: pLVX-CMV-Flag-PolB(K244A/T304I)-Puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             41..674
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    721..846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1343..1576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1761..1805
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2068..2183
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2241..2544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2545..2748
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2719
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2745..2769
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2864..2888
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2912..2935
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3963..3987)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4019..4593,4594..4596,4597..4605)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4186..4203)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4413..4432
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4625..5224
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4625..4644)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5121..5141
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5238..5826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5291..5311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5709..5720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6033..6666
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6794..6810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6794..6810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6807..6829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6818..6834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6842..6848,6849..6866,6867..6872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6887..6908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7025..7042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7196..7784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7276..7295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7955..8746,8747..8815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8578..8597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8816..8920)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8968..9102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9005..9024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9059..9078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
       61 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
      121 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
      181 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
      241 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
      301 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
      361 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
      421 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
      481 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      661 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      721 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      781 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      841 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      901 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      961 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1021 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1081 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1141 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     1201 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1261 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1321 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1381 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1441 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1501 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1561 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1621 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1681 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1741 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1801 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1861 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1921 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     1981 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2041 tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg ggggtacagt
     2101 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2161 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttat
     2221 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     2281 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2341 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2401 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2461 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2521 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2581 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2641 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2701 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2761 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2821 tactagagga tctatttccg gtgaattcct cgagactaga tcaacaagtt tgtacaaaaa
     2881 agcaggctcc gcggccgccc ccttcaccat ggactacaaa gacgatgacg ataaaggcat
     2941 gagcaaacgg aaggcgccgc aggagactct caacggggga atcaccgaca tgctcacaga
     3001 actcgcaaac tttgagaaga acgtgagcca agctatccac aagtacaatg cttacagaaa
     3061 agcagcatct gttatagcaa aatacccaca caaaataaag agtggagctg aagctaagaa
     3121 attgcctgga gtaggaacaa aaattgctga aaagattgat gagtttttag caactggaaa
     3181 attacgtaaa ctggaaaaga ttcggcagga tgatacgagt tcatccatca atttcctgac
     3241 tcgagttagt ggcattggtc catctgctgc aaggaagttt gtagatgaag gaattaaaac
     3301 actagaagat ctcagaaaaa atgaagataa attgaaccat catcagcgaa ttgggctgaa
     3361 atattttggg gactttgaaa aaagaattcc tcgtgaagag atgttacaaa tgcaagatat
     3421 tgtactaaat gaagttaaaa aagtggattc tgaatacatt gctacagtct gtggcagttt
     3481 cagaagaggt gcagagtcca gtggtgacat ggatgttctc ctgacccatc ccagcttcac
     3541 ttcagaatca accaaacagc caaaactgtt acatcaggtt gtggagcagt tacaaaaggt
     3601 tcattttatc acagataccc tgtcaaaggg tgagacaaag ttcatgggtg tttgccagct
     3661 tcccagtgca aatgatgaaa aagaatatcc acacagaaga attgatatca ggttgatacc
     3721 caaagatcag tattactgtg gtgttctcta tttcactggg agtgatattt tcaataagaa
     3781 tatgagggct catgccctag aaaagggttt cacaatcaat gagtacacca tccgtccctt
     3841 gggagtcatt ggagttgcag gagaacccct gccagtggat agtgaaaaag acatctttga
     3901 ttacatccag tggaaatacc gggaacccaa ggaccggagc gaatgaaagg gtgggcgcgc
     3961 cgacccagct ttcttgtaca aagtggttga tctagttcta gagcggccgc ggatcccgcc
     4021 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4081 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4141 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4201 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4261 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4321 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4381 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     4441 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4501 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     4561 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     4621 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     4681 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     4741 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4801 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     4861 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     4921 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     4981 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5041 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5101 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5161 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5221 ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5521 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5641 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5761 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5821 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     5881 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5941 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6001 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6061 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     6121 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     6181 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     6241 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     6301 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     6361 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     6421 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     6481 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6541 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6601 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6661 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6721 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct
     6781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8941 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9061 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9121 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     9181 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaa
//
