LOCUS       sequence-350709-        5730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CoTrIP plasmid with GLC3 promoter driving CFP's expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, 
            Yates JR 3rd, Zid BM
  TITLE     Rvb1/Rvb2 proteins couple transcription and translation during 
            glucose starvation.
  JOURNAL   eLife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965.
  PUBMED    36107469
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-09-15"; volume: "11. pii: 76965. doi"; pages: " 
            10.7554/eLife.76965"
COMMENT     Sequence Label: TalO8-GLC3-CFP
FEATURES             Location/Qualifiers
     source          1..5730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             555..1229
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      2624..2811
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2679..2703)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    3257..3273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3284..3300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3311..3327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3338..3354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3392..3408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3446..3462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3509..3525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3509..3525)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3522..3544)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3533..3549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3557..3563,3564..3581,3582..3587))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3602..3623
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3740..3757)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3911..4499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3991..4010)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4670..5461,5462..5530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5293..5312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5531..5635)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5703..5721
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg cggccgcggt cgaaaaaaga aaaggagagg
      481 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
      541 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
      601 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
      661 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
      721 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
      781 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
      841 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
      901 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
      961 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     1021 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     1081 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     1141 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     1201 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     1261 gtttgtgcac ttgcctgcag gccttttgaa aagcaagcat aaaagatcta aacataaaat
     1321 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     1381 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     1441 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgg tgaattctgt
     1501 agtctgtcat ccaggggaag ggcagggcac tcatcaacaa tgtgatagtc agaaagcaat
     1561 aatgttttgt attctgatga gctttcacta tctcttccgc aatacccctt tttgcctata
     1621 ttacggcatc atctttcccc gcgttctcgt gggggccaaa taagctttgt ccaaggggct
     1681 gttgcaacgg taaaggtagt cgagccaagt gacaccagca aacaaggaaa caaggaagca
     1741 aggaaggctt tccattttcc aataacgaca tgagtctatc tatggtgtat aaaagtggca
     1801 ggcttttcct acattttttt tccctgataa cttcctgtta ctatttaaga acaccaaacc
     1861 aagtataaag aaccgtcaag aataaaatgt ctaagggtga agaattgttc actggtgttg
     1921 ttccaatctt ggttgaattg gacggtgacg ttaacggtca caagttctct gtttctggtg
     1981 aaggtgaagg tgacgctact tacggtaagt tgactttgaa gttcatctgt actactggta
     2041 agttgccagt tccatggcca actttggtta ctactttgac ttggggtgtt caatgtttcg
     2101 ctagataccc agaccacatg aagcaacacg acttcttcaa gtctgctatg ccagaaggtt
     2161 acgttcaaga aagaactatc ttcttcaagg acgacggtaa ctacaagact agagctgaag
     2221 ttaagttcga aggtgacact ttggttaaca gaatcgaatt gaagggtatc gacttcaagg
     2281 aagacggtaa catcttgggt cacaagttgg aatacaacta catctctgac aacgtttaca
     2341 tcactgctga caagcaaaag aacggtatca aggctaactt caagatcaga cacaacatcg
     2401 aagacggtgg tgttcaattg gctgaccact accaacaaaa cactccaatc ggtgacggtc
     2461 cagttttgtt gccagacaac cactacttgt ctactcaatc taagttgtct aaggacccaa
     2521 acgaaaagag agaccacatg gttttgttgg aattcgttac tgctgctggt atcactttgg
     2581 gtatggacga attgtacaag taaggcgcgc cacttctaaa taagcgaatt tcttatgatt
     2641 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     2701 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     2761 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
     2821 tagggataac agggtaatat agatctgttt agcttgcctc gtcccccttt tctcggtctt
     2881 gcaaacaacc gccggcagct tagtatataa atacacatgt acatacctct ctccgtatcc
     2941 tcgtaatcat tttcttgtat ttatcgtctt ttcgctgtaa aaactttatc acacttatct
     3001 caaatacact tattaaccgc ttttactatt atcttctacg ctgacagtaa tatcaaacag
     3061 tgacacatat taaacacagt ggtttctttg cataaacacc atcagcctca agtcgtcaag
     3121 taaagatttc gtgttcatgc agatagataa caatctatat gttgataatt agcgttgcct
     3181 catcaatgcg agatccgttt aaccggaccc tagtgcactt accccacgtt cggtccactg
     3241 tgtgccgaac tagcaattgt gagcggataa caattactag caattgtgag cggataacaa
     3301 ttactagcaa ttgtgagcgg ataacaatta ctagcaattg tgagcggata acaattacta
     3361 gcaattgtga gcagataaca attactagca attgtgagcg gataacaatt actagcaatt
     3421 atgagcggat aacaattact agcaattgtg agcggataac aatactagtt ccttcactat
     3481 tttaacatgt ggcggccgcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3721 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3781 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3841 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3901 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3961 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4021 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4081 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4141 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4201 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4261 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4321 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4381 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4441 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4501 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4561 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4621 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5641 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     5701 aaaataggcg tatcacgagg ccctttcgtc
//
