LOCUS       sequence-350727-        6027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CoTrIP plasmid with HSP26 promoter driving CFP's expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, 
            Yates JR 3rd, Zid BM
  TITLE     Rvb1/Rvb2 proteins couple transcription and translation during 
            glucose starvation.
  JOURNAL   eLife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965.
  PUBMED    36107469
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-09-15"; volume: "11. pii: 76965. doi"; pages: " 
            10.7554/eLife.76965"
COMMENT     Sequence Label: TalO8-HSP26-CFP
FEATURES             Location/Qualifiers
     source          1..6027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             555..1229
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      2921..3108
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2976..3000)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    3554..3570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3581..3597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3608..3624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3635..3651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3689..3705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3743..3759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3806..3822)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3806..3822)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3819..3841)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3830..3846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3854..3860,3861..3878,3879..3884))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3899..3920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4037..4054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4208..4796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4288..4307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4967..5758,5759..5827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5590..5609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5828..5932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6000..6018
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg cggccgcggt cgaaaaaaga aaaggagagg
      481 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
      541 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
      601 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
      661 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
      721 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
      781 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
      841 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
      901 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
      961 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     1021 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     1081 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     1141 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     1201 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     1261 gtttgtgcac ttgcctgcag gccttttgaa aagcaagcat aaaagatcta aacataaaat
     1321 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     1381 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     1441 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc tccttgaaaa
     1501 caagactatg ggtctctctc ataagcctca gggggggacc ccaaaaaaat aacgcggcca
     1561 tcttgcatgc accgttgaac ctgtagctta cagtaagcca caattctctt accttcttgg
     1621 caatgtggca caaaataatc tggttatgtg tcttcatttg gtaatcactg ggatgttact
     1681 ggggcagcag caactccgtg tgtaccccta actccgtgtg tacccctaaa gaaccttgcc
     1741 tgtcaaggtg cattgttgga tcggaatagt aaccgtcttt acatgaacat ccacaaccaa
     1801 cgaaagtgct ttttcaagca ttgcttgatt tctagaaaga tcgatggtta ttccctcccc
     1861 cttatgcgtc caaaaatata gggtgctcgt aacagtaagg tattcgcact tagcgtgctc
     1921 gcaacacaaa attaagtaat atgcgagttt tagatgtcct tgcggatcta tgcacgttct
     1981 tgagtggtat ttcataacaa cggttctttt tcacccttat tcctaaacat ataaatagga
     2041 cctccattag ttagagatct gtttttaatc cattcacctt tcattctact ctcttatact
     2101 aataaaacca ccgataaaga tatatcagat ctctattaaa acaggtatcc aaaaaagcaa
     2161 acaaacaaac taaacaaatt aacatgtcta agggtgaaga attgttcact ggtgttgttc
     2221 caatcttggt tgaattggac ggtgacgtta acggtcacaa gttctctgtt tctggtgaag
     2281 gtgaaggtga cgctacttac ggtaagttga ctttgaagtt catctgtact actggtaagt
     2341 tgccagttcc atggccaact ttggttacta ctttgacttg gggtgttcaa tgtttcgcta
     2401 gatacccaga ccacatgaag caacacgact tcttcaagtc tgctatgcca gaaggttacg
     2461 ttcaagaaag aactatcttc ttcaaggacg acggtaacta caagactaga gctgaagtta
     2521 agttcgaagg tgacactttg gttaacagaa tcgaattgaa gggtatcgac ttcaaggaag
     2581 acggtaacat cttgggtcac aagttggaat acaactacat ctctgacaac gtttacatca
     2641 ctgctgacaa gcaaaagaac ggtatcaagg ctaacttcaa gatcagacac aacatcgaag
     2701 acggtggtgt tcaattggct gaccactacc aacaaaacac tccaatcggt gacggtccag
     2761 ttttgttgcc agacaaccac tacttgtcta ctcaatctaa gttgtctaag gacccaaacg
     2821 aaaagagaga ccacatggtt ttgttggaat tcgttactgc tgctggtatc actttgggta
     2881 tggacgaatt gtacaagtaa ggcgcgccac ttctaaataa gcgaatttct tatgatttat
     2941 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     3001 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     3061 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
     3121 ggataacagg gtaatataga tctgtttagc ttgcctcgtc ccccttttct cggtcttgca
     3181 aacaaccgcc ggcagcttag tatataaata cacatgtaca tacctctctc cgtatcctcg
     3241 taatcatttt cttgtattta tcgtcttttc gctgtaaaaa ctttatcaca cttatctcaa
     3301 atacacttat taaccgcttt tactattatc ttctacgctg acagtaatat caaacagtga
     3361 cacatattaa acacagtggt ttctttgcat aaacaccatc agcctcaagt cgtcaagtaa
     3421 agatttcgtg ttcatgcaga tagataacaa tctatatgtt gataattagc gttgcctcat
     3481 caatgcgaga tccgtttaac cggaccctag tgcacttacc ccacgttcgg tccactgtgt
     3541 gccgaactag caattgtgag cggataacaa ttactagcaa ttgtgagcgg ataacaatta
     3601 ctagcaattg tgagcggata acaattacta gcaattgtga gcggataaca attactagca
     3661 attgtgagca gataacaatt actagcaatt gtgagcggat aacaattact agcaattatg
     3721 agcggataac aattactagc aattgtgagc ggataacaat actagttcct tcactatttt
     3781 aacatgtggc ggccgcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3841 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3901 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3961 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4021 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4081 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4141 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4381 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4441 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4621 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5281 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5581 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5941 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6001 ataggcgtat cacgaggccc tttcgtc
//
