LOCUS       sequence-350729-        6343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CoTrIP plasmid with HSP30 promoter driving CFP's expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Chen YS, Hou W, Tracy S, Harvey AT, Harjono V, Xu F, Moresco JJ, 
            Yates JR 3rd, Zid BM
  TITLE     Rvb1/Rvb2 proteins couple transcription and translation during 
            glucose starvation.
  JOURNAL   eLife. 2022 Sep 15;11. pii: 76965. doi: 10.7554/eLife.76965.
  PUBMED    36107469
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2022-09-15"; volume: "11. pii: 76965. doi"; pages: " 
            10.7554/eLife.76965"
COMMENT     Sequence Label: TalO8-HSP30-CFP
FEATURES             Location/Qualifiers
     source          1..6343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             555..1229
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      3237..3424
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3292..3316)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    3870..3886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3897..3913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3924..3940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3951..3967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4005..4021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4059..4075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4122..4138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4122..4138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4135..4157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4146..4162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4170..4176,4177..4194,4195..4200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4215..4236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4353..4370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4524..5112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4604..4623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5283..6074,6075..6143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5906..5925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6144..6248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6316..6334
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg cggccgcggt cgaaaaaaga aaaggagagg
      481 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
      541 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
      601 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
      661 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
      721 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
      781 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
      841 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
      901 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
      961 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     1021 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     1081 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     1141 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     1201 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     1261 gtttgtgcac ttgcctgcag gccttttgaa aagcaagcat aaaagatcta aacataaaat
     1321 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     1381 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     1441 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc ctttcttcaa
     1501 aagtagaaaa cttgagcact tatttcctgc gcatgtcata tgttaatttt cctcaactgc
     1561 gctgaatacg tcctgtcaat tcaaatatat cacgttgtga gcagccctga agaagaaaac
     1621 ctcaacagca gtattactat tacaatcaaa caactttagt gccgcgtgat accgggggtt
     1681 gaagtgggtg cattgagccg tattcttctt ccccgtaaga aagttgtgta tcctttttac
     1741 tgcgttgtaa tagcttctga aaacctaaaa aatgaacgct atgtagctca tatccgtttt
     1801 gcataagtaa gaataactac ttgtgcaggg tgccgaaagg gatggaaaac cgctgcagca
     1861 acccttgtta catacagtcg gatccatctg acttactttc cttgcgtctc cctgcgcgat
     1921 tttgttggcc attttccaga tcctctagaa tttttcaagg gtcgagccgt aggaggattc
     1981 tctcagaagg caaaaacgca tcgaaagcgt gctttgtaag aatatttggt atggctaaag
     2041 taagcaaagc catatcccga tcccgatccc gactcttatt ccgatccctt ccgccacatc
     2101 ctgcatgttt attcgaatac caaattagct catcttcgtt atttcatcat ccctttctgc
     2161 tatggcaagg acaagttttt ttctagcatc tcatcgaaaa ctttcctctc cctaattggc
     2221 caaagttttc atattcatca tcagttagaa agtataatat caatccctta cctcattaca
     2281 agttgtatca cactaaaaaa atcatatata agtctgtgag agtcttcaat tatttagcgt
     2341 aacacctatt cactttctaa tcttgtttct tgtttttaca ttctgcaata caacacaaca
     2401 acaaatatta actcaattat tattatttat aattacaaaa acaaaacaac aagtttgaga
     2461 ctttaatatc ttttgattac taaaaacaac aaatttcaaa tgtctaaggg tgaagaattg
     2521 ttcactggtg ttgttccaat cttggttgaa ttggacggtg acgttaacgg tcacaagttc
     2581 tctgtttctg gtgaaggtga aggtgacgct acttacggta agttgacttt gaagttcatc
     2641 tgtactactg gtaagttgcc agttccatgg ccaactttgg ttactacttt gacttggggt
     2701 gttcaatgtt tcgctagata cccagaccac atgaagcaac acgacttctt caagtctgct
     2761 atgccagaag gttacgttca agaaagaact atcttcttca aggacgacgg taactacaag
     2821 actagagctg aagttaagtt cgaaggtgac actttggtta acagaatcga attgaagggt
     2881 atcgacttca aggaagacgg taacatcttg ggtcacaagt tggaatacaa ctacatctct
     2941 gacaacgttt acatcactgc tgacaagcaa aagaacggta tcaaggctaa cttcaagatc
     3001 agacacaaca tcgaagacgg tggtgttcaa ttggctgacc actaccaaca aaacactcca
     3061 atcggtgacg gtccagtttt gttgccagac aaccactact tgtctactca atctaagttg
     3121 tctaaggacc caaacgaaaa gagagaccac atggttttgt tggaattcgt tactgctgct
     3181 ggtatcactt tgggtatgga cgaattgtac aagtaaggcg cgccacttct aaataagcga
     3241 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     3301 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     3361 tgtaggtcag gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta
     3421 ccggcagatc cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccc
     3481 ttttctcggt cttgcaaaca accgccggca gcttagtata taaatacaca tgtacatacc
     3541 tctctccgta tcctcgtaat cattttcttg tatttatcgt cttttcgctg taaaaacttt
     3601 atcacactta tctcaaatac acttattaac cgcttttact attatcttct acgctgacag
     3661 taatatcaaa cagtgacaca tattaaacac agtggtttct ttgcataaac accatcagcc
     3721 tcaagtcgtc aagtaaagat ttcgtgttca tgcagataga taacaatcta tatgttgata
     3781 attagcgttg cctcatcaat gcgagatccg tttaaccgga ccctagtgca cttaccccac
     3841 gttcggtcca ctgtgtgccg aactagcaat tgtgagcgga taacaattac tagcaattgt
     3901 gagcggataa caattactag caattgtgag cggataacaa ttactagcaa ttgtgagcgg
     3961 ataacaatta ctagcaattg tgagcagata acaattacta gcaattgtga gcggataaca
     4021 attactagca attatgagcg gataacaatt actagcaatt gtgagcggat aacaatacta
     4081 gttccttcac tattttaaca tgtggcggcc gcgtaatcat ggtcatagct gtttcctgtg
     4141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4921 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6241 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     6301 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
