LOCUS       sequence-35074-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: GST-TEV-FUS-267-526aa
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    313..337
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtagacgagt catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct gccatatgta
      121 tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc gctcacaatt
      181 cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg attactttct
      241 gttcgactta agcattatgc ggccgcaagc ttgtcgacct gcaggcgcgc cgagctcgaa
      301 ttcggatcat caaccacttt gtacaagaaa gctgggtcat acggcctctc cctgcgatcc
      361 tgtctgtgct cacccctgga atccatcttg ccagggccaa agccacctct gtccccacca
      421 ccccggcccc ctcggaagcc tccacggtcc ccgccgcggc cccggtagcc gcctcgatca
      481 tagcctcctc tgccaccacg acgatcatcc ccgtagttac cccccatgtg agagccacct
      541 ggtccccctc ctgggccatc tggtttaggg gccttacact ggttgcattc attcctccaa
      601 gagaagttca tattctcaca ggtgggatta ggacacttcc agtcaccagc tcgctgctgt
      661 cctccaccgc caccacctcc actgggaaat cctcctcggc caccaccacc actgccacca
      721 cctccatagc ctccacggcc catgggtcct cctcgccctc ggcctccacg accattgcca
      781 ccaccccgat taaagtctgc ccggcgagta gcaaatgaga ccttgatagg atttccggag
      841 aattctttac catcaaacca gtcaatagct gctttagctg aaggtggggt catcaaaaaa
      901 agaccgttgc ctctcccttc agcttgnc
//
