LOCUS       sequence-350748-       18365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination Expression vector for dicot plant prime editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read 
            AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ
  TITLE     Spelling Changes and Fluorescent Tagging With Prime Editing Vectors 
            for Plants.
  JOURNAL   Front Genome Ed. 2021 Mar 4;3:617553. doi: 
            10.3389/fgeed.2021.617553. eCollection 2021.
  PUBMED    34713247
REFERENCE   2  (bases 1 to 18365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-03-4"; volume: "3:617553. doi"; pages: " 
            10.3389/fgeed.2021.617553. eCollection 2021"
COMMENT     Sequence Label: pPPED
FEATURES             Location/Qualifiers
     source          1..18365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1224..1853
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2282..3355
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3421..3615
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3851..3873)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3959..4099
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4014..4033
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4114..4131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4285..4873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4365..4384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4968..5783)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4968..5783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5691..5710
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5775..5794
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    6076..6100
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6277..6298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6313..6318,6319..6336,6337..6343)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6351..6367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6356..6378
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6375..6391
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6375..6391
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(6913..7707)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7024..7043)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7634..7653
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        8853..9529
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9466..9486
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9581..9601
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9608..12121,12122..12124,12125..13708)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             15896..15916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      15983..16235
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(16276..16293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16276..16292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16285..16307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    16491..16615
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(16652..16657,16658..16675,16676..16682)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             16736..17395
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(16802..16821)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             17737..18042
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     17921..17940
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(18083..18207)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    18234..18258
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggatcaaagt actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg
       61 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
      121 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
      181 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
      241 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
      301 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
      361 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
      421 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
      481 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
      541 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
      601 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
      661 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
      721 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
      781 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
      841 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
      901 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
      961 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     1021 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     1081 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     1141 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     1201 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     1261 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     1321 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     1381 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     1441 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     1501 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     1561 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     1621 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     1681 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     1741 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     1801 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     1861 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     1921 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     1981 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     2041 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     2101 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     2161 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     2221 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     2281 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     2341 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     2401 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     2461 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     2521 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     2581 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     2641 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     2701 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     2761 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     2821 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     2881 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     2941 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     3001 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     3061 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     3121 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     3181 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     3241 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     3301 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     3361 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     3421 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     3481 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     3541 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     3601 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     3661 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     3721 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     3781 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     3841 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3901 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     3961 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     4021 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4081 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4921 aaactcacgt taagggattt tggtcatgca ggatcatgaa ttaattctta gaaaaactca
     4981 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     5041 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     5101 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     5161 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     5221 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     5281 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     5341 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     5401 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     5461 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     5521 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     5581 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     5641 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     5701 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     5761 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     5821 tttattgttc atgatctgga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     5881 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     5941 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     6001 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     6061 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     6121 taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattgc tctagccaat
     6181 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     6241 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6301 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6361 ataacaattt cacacaggaa acagctatga catgattacg aatccttgac tagagggtag
     6421 gcgcggctta attaagcgct taattaagcg cttaattaag cgcttaataa gccgcgccgt
     6481 accccaatag tctcgacaga cacatagcac ctaacattat tgcttaaatt tactattttt
     6541 ttactatatt atactcaacc caatgagcat aaagactgta aaatctcaaa ttcctgagaa
     6601 gcatatttat cgatcccaca gacttgatag ttccataatc catacgctgc agccaaattg
     6661 ctagtgtgtt gaacatttaa cacgtagaga actagaaaag atataaaact aagattgata
     6721 tccaaaatag acgagaacaa taagcaaaaa ctcttagttt tgaaataaat caacaatccc
     6781 gagggttgtc acatacatca aaaacgaaaa tccatatagc aaaaaaaact ctaaattacc
     6841 gttcgacaaa aagagaaaac tgataggaca tttgctaaac attaaaatca aaatttggat
     6901 caagccgaat tctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     6961 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     7021 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     7081 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     7141 gccatgggtc acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     7201 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     7261 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     7321 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     7381 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     7441 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     7501 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     7561 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     7621 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     7681 tgcgtgcaat ccatcttgtt caatcatctc gagacgtacc gcgcgatgca ttcgaagatc
     7741 gatccttcgc ctggaggaga gaaatcagtg gcgctgcggc ttttagggtt tctttgttga
     7801 tggaatgaga gtgtaagctc tgccagtgcc actttattag ggttttacaa gcccttttct
     7861 tcgtaattgg gcctgacatt ttgtgccact tgggccttta gagatgaaaa tgtatattgg
     7921 gcttaagttg acttgaagga taaattagtt taggatatta cgttttttat gagaattggt
     7981 gtgtcggata catgtatatg atgcattcaa atatatgtat tctagataca tttaagttta
     8041 gatacaatct aaaatgtgtc tttaattaca ggactgtaac taaaatactt aatgtaagaa
     8101 gaatattact cctttaatag cttttgagta tatctagtct aacatctttt aaaaaagtct
     8161 aatttctttc atttattttt cgagcaatag caaagtgcat aattattttt ttcttctaga
     8221 aattcagatt tgtttctcta aattttgaga ttcttttctc aattttgtat gtctagagaa
     8281 caatgtgtat ttttcactct agttggttgt tactttgttg aatgttctga taaaagtata
     8341 ttgttatttc tgaagtagat ataaaccttc atttggaaat tatacataaa tcaaaatcgt
     8401 taattatcta gatcaagata tatgcccttt tcctaatgta tttgatacat gcacctaatt
     8461 tcactagatg tatcttttct attttttaaa ttatgaatag ttaatttttt ccatatgtgt
     8521 atttgataca tacttcatga ctttaaaaaa ttaattatat accagatata tgtatttaaa
     8581 atttgttatg tatttaaagt atgtatatga ttattcgata ttaatctctt cgatgaaatt
     8641 taaatcgata actatgtgct ttggatcgat ctgcccacta gtgagtcgta ttacaattca
     8701 tgactctctt aaggtagcca aaatcgatat ctagggataa cagggtaatc gatcactaga
     8761 attcggcgcg ccgggcccaa catggtggag cacgacactc tcgtctactc caagaatatc
     8821 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
     8881 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
     8941 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     9001 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     9061 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
     9121 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     9181 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     9241 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     9301 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     9361 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     9421 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     9481 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
     9541 tctctctaca aatctatctc tctcgaccgc tagcatggca cctaagaaaa agaggaaggt
     9601 tggtattgat aagaagtact ctatcggact cgatatcgga actaactctg tgggatgggc
     9661 tgtgatcacc gatgagtaca aggtgccatc taagaagttc aaggttctcg gaaacaccga
     9721 taggcactct atcaagaaaa accttatcgg tgctctcctc ttcgattctg gtgaaactgc
     9781 tgaggctacc agactcaaga gaaccgctag aagaaggtac accagaagaa agaacaggat
     9841 ctgctacctc caagagatct tctctaacga gatggctaaa gtggatgatt cattcttcca
     9901 caggctcgaa gagtcattcc tcgtggaaga agataagaag cacgagaggc accctatctt
     9961 cggaaacatc gttgatgagg tggcatacca cgagaagtac cctactatct accacctcag
    10021 aaagaagctc gttgattcta ctgataaggc tgatctcagg ctcatctacc tcgctctcgc
    10081 tcacatgatc aagttcagag gacacttcct catcgagggt gatctcaacc ctgataactc
    10141 tgatgtggat aagttgttca tccagctcgt gcagacctac aaccagcttt tcgaagagaa
    10201 ccctatcaac gcttcaggtg tggatgctaa ggctatcctc tctgctaggc tctctaagtc
    10261 aagaaggctt gagaacctca ttgctcagct ccctggtgag aagaagaacg gacttttcgg
    10321 aaacttgatc gctctctctc tcggactcac ccctaacttc aagtctaact tcgatctcgc
    10381 tgaggatgca aagctccagc tctcaaagga tacctacgat gatgatctcg ataacctcct
    10441 cgctcagatc ggagatcagt acgctgattt gttcctcgct gctaagaacc tctctgatgc
    10501 tatcctcctc agtgatatcc tcagagtgaa caccgagatc accaaggctc cactctcagc
    10561 ttctatgatc aagagatacg atgagcacca ccaggatctc acacttctca aggctcttgt
    10621 tagacagcag ctcccagaga agtacaaaga gattttcttc gatcagtcta agaacggata
    10681 cgctggttac atcgatggtg gtgcatctca agaagagttc tacaagttca tcaagcctat
    10741 cctcgagaag atggatggaa ccgaggaact cctcgtgaag ctcaatagag aggatcttct
    10801 cagaaagcag aggaccttcg ataacggatc tatccctcat cagatccacc tcggagagtt
    10861 gcacgctatc cttagaaggc aagaggattt ctacccattc ctcaaggata acagggaaaa
    10921 gattgagaag attctcacct tcagaatccc ttactacgtg ggacctctcg ctagaggaaa
    10981 ctcaagattc gcttggatga ccagaaagtc tgaggaaacc atcacccctt ggaacttcga
    11041 agaggtggtg gataagggtg ctagtgctca gtctttcatc gagaggatga ccaacttcga
    11101 taagaacctt ccaaacgaga aggtgctccc taagcactct ttgctctacg agtacttcac
    11161 cgtgtacaac gagttgacca aggttaagta cgtgaccgag ggaatgagga agcctgcttt
    11221 tttgtcaggt gagcaaaaga aggctatcgt tgatctcttg ttcaagacca acagaaaggt
    11281 gaccgtgaag cagctcaaag aggattactt caagaaaatc gagtgcttcg attcagttga
    11341 gatttctggt gttgaggata ggttcaacgc atctctcgga acctaccacg atctcctcaa
    11401 gatcattaag gataaggatt tcttggataa cgaggaaaac gaggatatct tggaggatat
    11461 cgttcttacc ctcaccctct ttgaagatag agagatgatt gaagaaaggc tcaagaccta
    11521 cgctcatctc ttcgatgata aggtgatgaa gcagttgaag agaagaagat acactggttg
    11581 gggaaggctc tcaagaaagc tcattaacgg aatcagggat aagcagtctg gaaagacaat
    11641 ccttgatttc ctcaagtctg atggattcgc taacagaaac ttcatgcagc tcatccacga
    11701 tgattctctc acctttaaag aggatatcca gaaggctcag gtttcaggac agggtgatag
    11761 tctccatgag catatcgcta acctcgctgg atctcctgca atcaagaagg gaatcctcca
    11821 gactgtgaag gttgtggatg agttggtgaa ggtgatggga aggcataagc ctgagaacat
    11881 cgtgatcgaa atggctagag agaaccagac cactcagaag ggacagaaga actctaggga
    11941 aaggatgaag aggatcgagg aaggtatcaa agagcttgga tctcagatcc tcaaagagca
    12001 ccctgttgag aacactcagc tccagaatga gaagctctac ctctactacc tccagaacgg
    12061 aagggatatg tatgtggatc aagagttgga tatcaacagg ctctctgatt acgatgttga
    12121 tgctatcgtg ccacagtcat tcttgaagga tgattctatc gataacaagg tgctcaccag
    12181 gtctgataag aacaggggta agagtgataa cgtgccaagt gaagaggttg tgaagaaaat
    12241 gaagaactat tggaggcagc tcctcaacgc taagctcatc actcagagaa agttcgataa
    12301 cttgactaag gctgagaggg gaggactctc tgaattggat aaggcaggat tcatcaagag
    12361 gcagcttgtg gaaaccaggc agatcactaa gcacgttgca cagatcctcg attctaggat
    12421 gaacaccaag tacgatgaga acgataagtt gatcagggaa gtgaaggtta tcaccctcaa
    12481 gtcaaagctc gtgtctgatt tcagaaagga tttccaattc tacaaggtga gggaaatcaa
    12541 caactaccac cacgctcacg atgcttacct taacgctgtt gttggaaccg ctctcatcaa
    12601 gaagtatcct aagctcgagt cagagttcgt gtacggtgat tacaaggtgt acgatgtgag
    12661 gaagatgatc gctaagtctg agcaagagat cggaaaggct accgctaagt atttcttcta
    12721 ctctaacatc atgaatttct tcaagaccga gattaccctc gctaacggtg agatcagaaa
    12781 gaggccactc atcgagacaa acggtgaaac aggtgagatc gtgtgggata agggaaggga
    12841 tttcgctacc gttagaaagg tgctctctat gccacaggtg aacatcgtta agaaaaccga
    12901 ggtgcagacc ggtggattct ctaaagagtc tatcctccct aagaggaact ctgataagct
    12961 cattgctagg aagaaggatt gggaccctaa gaaatacggt ggtttcgatt ctcctaccgt
    13021 ggcttactct gttctcgttg tggctaaggt tgagaaggga aagagtaaga agctcaagtc
    13081 tgttaaggaa cttctcggaa tcactatcat ggaaaggtca tctttcgaga agaacccaat
    13141 cgatttcctc gaggctaagg gatacaaaga ggttaagaag gatctcatca tcaagctccc
    13201 aaagtactca ctcttcgaac tcgagaacgg tagaaagagg atgctcgctt ctgctggtga
    13261 gcttcaaaag ggaaacgagc ttgctctccc atctaagtac gttaactttc tttacctcgc
    13321 ttctcactac gagaagttga agggatctcc agaagataac gagcagaagc aacttttcgt
    13381 tgagcagcac aagcactact tggatgagat catcgagcag atctctgagt tctctaaaag
    13441 ggtgatcctc gctgatgcaa acctcgataa ggtgttgtct gcttacaaca agcacagaga
    13501 taagcctatc agggaacagg cagagaacat catccatctc ttcaccctta ccaacctcgg
    13561 tgctcctgct gctttcaagt acttcgatac aaccatcgat aggaagagat acacctctac
    13621 caaagaagtg ctcgatgcta ccctcatcca tcagtctatc actggactct acgagactag
    13681 gatcgatctc tcacagctcg gtggtgatct cagtggtggg agcagtggtg gatcatcagg
    13741 gagtgagact ccaggtacaa gtgaaagcgc tactcctgaa tcttcaggag gttcctcagg
    13801 aggaagttct actcttaaca tcgaggacga gtatcgtctc cacgagacca gcaaggaacc
    13861 tgatgtatct ctaggatcaa cttggctcag tgatttcccc caggcatggg cagaaacagg
    13921 tggcatgggt ttggctgtac gtcaagcacc acttataatt cctctaaaag caacaagcac
    13981 ccccgtcagt attaagcagt acccaatgag tcaggaggct agattgggca ttaagccaca
    14041 catacagaga cttttagatc aaggcattct ggtaccctgt cagtccccct ggaacacacc
    14101 actgctgcct gttaagaaac caggaaccaa cgattaccga ccagttcagg acttgcgaga
    14161 agtgaacaaa agggtcgagg atatccaccc taccgtgcct aatccataca accttttatc
    14221 tggccttcct ccaagccatc aatggtacac cgttctagat ttgaaagatg cattcttttg
    14281 tctgcgactc caccccacct cacagcccct tttcgccttc gagtggagag accctgaaat
    14341 gggtatcagc ggccaactta cttggactag gttaccccag ggatttaaaa acagtccaac
    14401 cctattcaat gaggcattgc acagggacct ggcagatttt cgtatacaac accccgatct
    14461 aatattgtta caatacgtgg acgaccttct cctagctgca acaagcgagt tagattgtca
    14521 gcaaggaaca agggctcttt tgcaaacact aggaaacttg gggtatcgtg ctagtgccaa
    14581 aaaagctcag atttgtcaaa agcaggtaaa gtacttaggg tatttactaa aggaaggtca
    14641 gagatggtta accgaagccc gaaaggaaac tgtaatgggg caaccaactc ctaaaactcc
    14701 cagacaactc agggagttct tgggcaaagc agggttttgt agacttttca tacccggctt
    14761 tgctgaaatg gcagcacccc tgtatccctt gacaaagcct gggacactat ttaactgggg
    14821 gccagaccag cagaaggcat accaagaaat aaagcaagca ctactcaccg cacctgccct
    14881 aggcctgcct gatctgacta aaccatttga actcttcgta gatgagaaac aaggctacgc
    14941 taaaggtgtg ctgacacaaa aattgggacc ctggcgacga ccagtcgcct acctcagcaa
    15001 gaaattagac cccgtggccg ccggttggcc accatgctta cgaatggtgg cagcaatcgc
    15061 cgtcctgaca aaggatgcag gcaaacttac catgggccaa cctctagtca tcttagcacc
    15121 ccatgccgtt gaggctctcg taaagcagcc acctgataga tggctcagta acgctcgaat
    15181 gacccactat caagccctcc tcttggatac cgacagagtc cagttcggac ccgtagtagc
    15241 acttaaccca gcaacactgc tacccctccc tgaggaaggg ctacaacata actgcctaga
    15301 cattttggcc gaggcacatg ggacacgtcc agacttgaca gatcagccct taccagacgc
    15361 cgaccacaca tggtacacag acggaagttc actcttgcag gagggtcaga ggaaggccgg
    15421 ggctgctgtg actaccgaga ccgaagttat ttgggctaag gccctaccag ccggcacctc
    15481 tgcacagaga gcagagttaa ttgcactaac ccaggccctc aaaatggccg aaggtaaaaa
    15541 actaaatgtc tatacagatt cacgatatgc ttttgcaaca gcccatatac atggcgaaat
    15601 ttataggagg agggggtggt taacatcaga gggaaaagag ataaaaaaca aggacgaaat
    15661 ccttgcattg ctaaaggctt tattccttcc caagcgttta agcataatcc actgtcccgg
    15721 tcatcagaag ggtcactccg ccgaggcaag aggcaaccga atggctgacc aagccgcaag
    15781 gaaggccgca atcactgaga ccccagacac ctctactcta ctaatcgaaa acagctcccc
    15841 ttcaggtgga agcaagagaa ccgccgacgg cagtgaattc gaaagcaggg ctgaccccaa
    15901 gaagaagagg aaggtgtgac cgcggatatc tgcagaagct tacgcgtcga cgtctcgagg
    15961 ccggccgagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
    16021 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
    16081 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
    16141 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
    16201 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgatatcta gggataacag
    16261 ggtaataagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    16321 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
    16381 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gagcttgagc
    16441 ttggatcaga ttgtcgtttc ccgccttcag tttggactct agagttatca acaagtttgt
    16501 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
    16561 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
    16621 ccctcgaggg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
    16681 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
    16741 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
    16801 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
    16861 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
    16921 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
    16981 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
    17041 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
    17101 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
    17161 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
    17221 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
    17281 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
    17341 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
    17401 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
    17461 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
    17521 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
    17581 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
    17641 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
    17701 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
    17761 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
    17821 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
    17881 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
    17941 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
    18001 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
    18061 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
    18121 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
    18181 tcgttcagct ttcttgtaca aagtggttga taacagcgga aactatcagt gtttgacagg
    18241 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
    18301 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
    18361 cctcg
//
