LOCUS       sequence-35075-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: GST-TEV-FUS-267-526aa
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcggatctgg ctagcgagaa tctttatttt cagggatcat caacaagttt gtacaaaaaa
       61 gcaggcttcg aaggagataa caaaatgggc cctcgggacc aaggatcacg tcatgactcc
      121 gaacaggata attcagacaa caacaccatc tttgtgcaag gcctgggtga gaatgttaca
      181 attgagtctg tggctgatta cttcaagcag attggtatta ttaagacaaa caagaaaacg
      241 ggacagccca tgattaattt gtacacagac agggaaactg gcaagctgaa gggagaggca
      301 acggtctctt ttgatgaccc accttcagct aaagcagcta ttgactggtt tgatggtaaa
      361 gaattctccg gaaatcctat caaggtctca tttgctactc gccgggcaga ctttaatcgg
      421 ggtggtggca atggtcgtgg aggccgaggg cgaggaggac ccatgggccg tggaggctat
      481 ggaggtggtg gcagtggtgg tggtggccga ggaggatttc ccagtggagg tggtggcggt
      541 ggaggacagc agcgagctgg tgactggaag tgtcctaatc ccacctgtga gaatatgaac
      601 ttctcttgga ggaatgaatg caaccagtgt aaggccccta aaccagatgg cccaggaggg
      661 ggaccaggtg gctctcacat ggggggtaac tacggggatg atcgtcgtgg tggcagagga
      721 ggctatgatc gaggcggcta ccggggccgc ggcggggacc gtggaggctt ccgagggggc
      781 cggggtggtg gggacagagg tggctttggc cctggcaaga tggattccag gggtgagcac
      841 agacaggatc gca
//
