LOCUS       sequence-35076-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: GST-TEV-FUS-1-373aa
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..55)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    164..188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(188..207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(189..207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    319..343
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgctagtaga cgagtccatg tgctggcgtt caaatttcgc agcagcggtt tctttaccag
       61 actcgagggt accgacgtca gcgatcgcgt ggccggccga tatccaattg agatctgcca
      121 tatgtatatc tccttcttat acttaactaa tatactaaga tggggaattg ttatccgctc
      181 acaattcccc tatagtgagt cgtattaatt tcgattatgc ggccgtgtac aatacgatta
      241 ctttctgttc gacttaagca ttatgcggcc gcaagcttgt cgacctgcag gcgcgccgag
      301 ctcgaattcg gatcatcaac cactttgtac aagaaagctg ggtcgtctgc ccggcgagta
      361 gcaaatgaga ccttgatagg atttccggag aattctttac catcaaacca gtcaatagct
      421 gctttagctg aaggtgggtc atcaaaagag accgttgcct ctcccttcag cttgccagtt
      481 tccctgtctg tgtacaaatt aatcatgggc tgtcccgttt tcttgtttgt cttaataata
      541 ccaatctgct tgaagtaatc agccacagac tcaattgtaa cattctcacc caggccttgc
      601 acaaagatgg tgttgttgtc tgaattatcc tgttcggagt catgacgtga tccttggtcc
      661 cgagggccac caaatttatt gaagccacca cggtcacttc cgcccatgcc acctctgcct
      721 ccacggccac ctccacgacc tctgggttca tagccaccac tgctgcggtt gtaaccacca
      781 ccgccgccgc cgccgccgcc accactgcca cccctgccgc ggcctccacg gtcctgctgt
      841 ccatagccac cgctgccacc tccaccactc tggtcttgat tgccataacc
//
