LOCUS       sequence-35077-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: GST-TEV-FUS-1-373aa
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             465..476
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcggatctgg ctagcgagaa tctttatttt cagggatcat caacaagttt gtacaaaaaa
       61 gcaggcttcg aaggagataa caaaatggcc tcaaacgatt atacccaaca agcaacccaa
      121 agctatgggg cctaccccac ccagcccggg cagggctatt cccagcagag cagtcagccc
      181 tacggacagc agagttacag tggttatagc cagtccacgg acacttcagg ctatggccag
      241 agcagctatt cttcttatgg ccagagccag aacacaggct atggaactca gtcaactccc
      301 cagggatatg gctcgactgg cggctatggc agtagccaga gctcccaatc gtcttacggg
      361 cagcagtcct cctatcctgg ctatggccag cagccagctc ccagcagcac ctcgggaagt
      421 tacggtagca gttctcagag cagcagctat gggcagcccc agagtgggag ctacagccag
      481 cagcctagct atggtggaca gcagcaaagc tatggacagc agcaaagcta taatccccct
      541 cagggctatg gacagcagaa ccagtacaac agcagcagtg gtggtggagg tggaggtgga
      601 ggtggaggta actatggcca agatcaatcc tccatgagta gtggtggtgg cagtggtggc
      661 ggttatggca atcaagacca gagtggtgga ggtggcagcg gtggctatgg acagcaggac
      721 cgtggaggcc gcggcagggg tggcagtggt ggcggcggcg gcggcggcgg tggtggttac
      781 aaccgcagca gtggtggcta tgaacccaga ggtcg
//
