LOCUS       sequence-35078-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: GST-TEV-FUS-1-413aa
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    169..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    324..348
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc
      121 tgccatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc
      181 cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac
      241 gattactttc tgttcgactt aagcattatg cggccgcaag cttgtcgacc tgcaggcgcg
      301 ccgagctcga attcggatca tcaaccactt tgtacaagaa agctgggtct ccactgggaa
      361 atcctcctcg gccaccacca ccactgccac cacctccata gcctccacgg cccatgggtc
      421 ctcctcgccc tcggcctcca cgaccattgc caccaccccg attaaagtct gcccggcgag
      481 tagcaaatga gaccttgata ggatttccgg agaattcttt accatcaaac cagtcaatag
      541 ctgctttagc tgaaggtggg tcatcaaaag agaccgttgc ctctcccttc agcttgccag
      601 tttccctgtc tgtgtacaaa ttaatcatgg gctgtcccgt tttcttgttt gtcttaataa
      661 taccaatctg cttgaagtaa tcagccacag actcaattgt aacattctca cccaggcctt
      721 gcacaaagat ggtgttgttg tctgaattat cctgttcgga gtcatgacgt gatccttggt
      781 cccgagggcc accaaattta ttgaagccac cacggtcact tccgcccatg ccacctctgc
      841 ctccacggcc acctccacga cctctgggtt catagccacc actgctgcgg ttgtaa
//
