LOCUS       sequence-350787-        9782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Giacomelli AO, Yang X, Lintner RE, McFarland JM, Duby M, Kim J, 
            Howard TP, Takeda DY, Ly SH, Kim E, Gannon HS, Hurhula B, Sharpe T, 
            Goodale A, Fritchman B, Steelman S, Vazquez F, Tsherniak A, Aguirre 
            AJ, Doench JG, Piccioni F, Roberts CWM, Meyerson M, Getz G, 
            Johannessen CM, Root DE, Hahn WC
  TITLE     Mutational processes shape the landscape of TP53 mutations in human 
            cancer.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1381-1387. doi: 
            10.1038/s41588-018-0204-y.
  PUBMED    30224644
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2018-10"; volume: "50"; issue: "10"; pages: 
            "1381-1387"
COMMENT     Sequence Label: pLX313-Renilla luciferase
FEATURES             Location/Qualifiers
     source          1..9782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             24..257
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    335..456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(372..391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     426..445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      496..631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(651..670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(652..670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(680..697)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(680..696)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(689..711)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      838..1293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(925..944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1135..1156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1319..1423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1424..1492,1493..2284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1642..1661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2455..3043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2944..2963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3197..3214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3331..3352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3367..3372,3373..3390,3391..3397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3405..3421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3410..3432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3429..3445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3429..3445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3464..3484
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3466..3484
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3512..3738
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3739..3919
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3966..4091
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4584..4817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5002..5046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5277..5487
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5299..5495
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5346..5481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5517..6536
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    6607..6724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6862..8040
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7093..8031
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7988..8008
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8060..8084
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8084..9019
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(9022..9046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9054..9095
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9146..9734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9199..9219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9617..9628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
       61 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      121 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      181 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      241 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
      301 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
      361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      421 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
      481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      601 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
      661 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      721 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      781 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      961 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1021 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1081 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1141 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1201 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1261 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     1321 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1381 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1441 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1501 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1561 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1621 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1681 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1741 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1801 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1861 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1921 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1981 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2041 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2101 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2161 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2221 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2281 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2341 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2521 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2581 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2701 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2761 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2821 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2881 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2941 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3001 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3061 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3121 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3181 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3241 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3301 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3361 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3421 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     3481 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     3541 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     3601 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     3661 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     3721 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3901 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     3961 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4021 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4081 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4141 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4201 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4261 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4321 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4381 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4441 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4501 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4561 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4621 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4681 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4741 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4801 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4861 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4921 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     4981 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     5041 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     5101 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     5161 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     5221 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5281 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
     5341 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5401 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5461 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccatga
     5521 aaaagcctga actcaccgct acctctgtcg agaagtttct gatcgaaaag ttcgacagcg
     5581 tctccgacct gatgcagctc tccgagggcg aagaatctcg ggctttcagc ttcgatgtgg
     5641 gagggcgtgg atatgtcctg cgggtgaata gctgcgccga tggtttctac aaagatcgct
     5701 atgtttatcg gcactttgca tccgccgctc tccctattcc cgaagtgctt gacattgggg
     5761 agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc accttgcaag
     5821 acctgcctga aaccgaactg cccgctgttc tccagcccgt cgccgaggcc atggatgcca
     5881 tcgctgccgc cgatcttagc cagaccagcg ggttcggccc attcggacct caaggaatcg
     5941 gtcaatacac tacatggcgc gatttcatct gcgctattgc tgatccccat gtgtatcact
     6001 ggcaaactgt gatggacgac accgtcagtg cctccgtcgc ccaggctctc gatgagctga
     6061 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgccgat ttcggctcca
     6121 acaatgtcct gaccgacaat ggccgcataa cagccgtcat tgactggagc gaggccatgt
     6181 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccctgg ttggcttgta
     6241 tggagcagca gacccgctac ttcgagcgga ggcatcccga gcttgcagga tctcctcggc
     6301 tccgggctta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     6361 atttcgatga tgcagcttgg gctcagggtc gctgcgacgc aatcgtccgg tccggagccg
     6421 ggactgtcgg gcgtacacaa atcgcccgca gaagcgctgc cgtctggacc gatggctgtg
     6481 tggaagtgct cgccgatagt ggaaacagac gccccagcac tcgtcctagg gcaaaggatc
     6541 tgcagtaatg aggcgcgccg ttaacgaatt ctagatcttg agacaaatgg cagtattcat
     6601 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     6661 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     6721 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg aggcctgcag
     6781 gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     6841 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     6901 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     6961 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     7021 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     7081 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     7141 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     7201 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     7261 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     7321 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     7381 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     7441 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     7501 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     7561 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     7621 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     7681 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     7741 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     7801 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     7861 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     7921 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     7981 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     8041 ggctagcatc gattgatcaa caagtttgta caaaaaagtt ggcatgactt cgaaagttta
     8101 tgatccagaa caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca
     8161 aatgaatgtt cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc
     8221 tgttattttt ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca
     8281 tattgagcca gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa
     8341 atctggtaat ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga
     8401 acttcttaat ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc
     8461 atttcattat agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt
     8521 agtagatgtg attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat
     8581 caaatctgaa gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt
     8641 gccatcaaaa atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt
     8701 caaagagaaa ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt
     8761 aaaaggtggt aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc
     8821 aagtgatgat ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat
     8881 tgttgaaggt gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt
     8941 ttcgcaagaa gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt
     9001 tctcaaaaat gaacaataaa cccccaactt tcttgtacaa agtggttgat atcggtaagc
     9061 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag
     9121 tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     9181 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     9241 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9301 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9361 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9421 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     9481 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     9541 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     9601 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9661 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     9721 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     9781 ca
//
